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Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).1 Publication

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei188Thioimide intermediateUniRule annotation1 Publication1
Active sitei195Proton donorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi89 – 90NADPH2
Nucleotide bindingi256 – 257NADPH2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.7.1.13. 6626.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:VC_0902
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001630621 – 281NADPH-dependent 7-cyano-7-deazaguanine reductaseAdd BLAST281

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi243277.VC0902.

Structurei

Secondary structure

1281
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi27 – 29Combined sources3
Beta strandi32 – 34Combined sources3
Helixi36 – 41Combined sources6
Beta strandi51 – 58Combined sources8
Beta strandi62 – 64Combined sources3
Beta strandi66 – 68Combined sources3
Beta strandi70 – 72Combined sources3
Beta strandi74 – 80Combined sources7
Beta strandi83 – 87Combined sources5
Helixi89 – 97Combined sources9
Turni98 – 101Combined sources4
Helixi107 – 122Combined sources16
Beta strandi127 – 131Combined sources5
Helixi133 – 136Combined sources4
Beta strandi145 – 148Combined sources4
Helixi163 – 166Combined sources4
Beta strandi170 – 188Combined sources19
Turni189 – 191Combined sources3
Beta strandi194 – 208Combined sources15
Helixi210 – 218Combined sources9
Turni219 – 222Combined sources4
Beta strandi223 – 225Combined sources3
Helixi227 – 242Combined sources16
Beta strandi245 – 253Combined sources9
Beta strandi259 – 268Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BP1X-ray1.53A/B/C/D1-281[»]
3RJ4X-ray1.75A/B1-281[»]
3RZPX-ray2.00A/B/C/D1-281[»]
3S19X-ray1.50A/B/C/D1-281[»]
3UXJX-ray1.40A/B/C/D1-281[»]
3UXVX-ray1.56A/B/C/D1-281[»]
4GHMX-ray1.62A/B1-281[»]
4IQIX-ray1.50A/B1-281[»]
ProteinModelPortaliQ9KTK0.
SMRiQ9KTK0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KTK0.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni87 – 89Substrate binding3
Regioni227 – 228Substrate binding2

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R1K. Bacteria.
COG0780. LUCA.
COG2904. LUCA.
KOiK06879.
OMAiQCVERIY.

Family and domain databases

HAMAPiMF_00817. QueF_type2. 1 hit.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KTK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKYSDAKEL ASLTLGKKTE YANQYDPSLL QPVPRSLNRN DLHLSATLPF
60 70 80 90 100
QGCDIWTLYE LSWLNQKGLP QVAIGEVSIP ATSANLIESK SFKLYLNSYN
110 120 130 140 150
QTRFASWDEV QTRLVHDLSA CAGETVTVNV KSLNEYTAEP IVTMQGECID
160 170 180 190 200
DQDIEIANYE FDDALLQGAA QGEEVSEVLH SHLLKSNCLI TNQPDWGSVE
210 220 230 240 250
IAYHGAKMNR EALLRYLVSF REHNEFHEQC VERIFTDIMR YCQPQSLTVY
260 270 280
ARYTRRGGLD INPFRSSHQS APNHNQRMAR Q
Length:281
Mass (Da):31,969
Last modified:November 22, 2005 - v2
Checksum:i9059809F7D2D5B02
GO

Sequence cautioni

The sequence AAF94064 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94064.1. Different initiation.
PIRiF82265.
RefSeqiNP_230549.2. NC_002505.1.
WP_000049898.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF94064; AAF94064; VC_0902.
GeneIDi2614193.
KEGGivch:VC0902.
PATRICi20080902. VBIVibCho83274_0859.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94064.1. Different initiation.
PIRiF82265.
RefSeqiNP_230549.2. NC_002505.1.
WP_000049898.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BP1X-ray1.53A/B/C/D1-281[»]
3RJ4X-ray1.75A/B1-281[»]
3RZPX-ray2.00A/B/C/D1-281[»]
3S19X-ray1.50A/B/C/D1-281[»]
3UXJX-ray1.40A/B/C/D1-281[»]
3UXVX-ray1.56A/B/C/D1-281[»]
4GHMX-ray1.62A/B1-281[»]
4IQIX-ray1.50A/B1-281[»]
ProteinModelPortaliQ9KTK0.
SMRiQ9KTK0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0902.

Protocols and materials databases

DNASUi2614193.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF94064; AAF94064; VC_0902.
GeneIDi2614193.
KEGGivch:VC0902.
PATRICi20080902. VBIVibCho83274_0859.

Phylogenomic databases

eggNOGiENOG4107R1K. Bacteria.
COG0780. LUCA.
COG2904. LUCA.
KOiK06879.
OMAiQCVERIY.

Enzyme and pathway databases

UniPathwayiUPA00392.
BRENDAi1.7.1.13. 6626.

Miscellaneous databases

EvolutionaryTraceiQ9KTK0.

Family and domain databases

HAMAPiMF_00817. QueF_type2. 1 hit.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQUEF_VIBCH
AccessioniPrimary (citable) accession number: Q9KTK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: November 2, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.