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Q9KTK0

- QUEF_VIBCH

UniProt

Q9KTK0 - QUEF_VIBCH

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Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).1 Publication

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei188 – 1881Thioimide intermediate1 PublicationUniRule annotation
Active sitei195 – 1951Proton donorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi89 – 902NADPH
Nucleotide bindingi256 – 2572NADPH

GO - Molecular functioni

  1. oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Source: InterPro
  2. preQ1 synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:VC_0902
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 281281NADPH-dependent 7-cyano-7-deazaguanine reductasePRO_0000163062Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 PublicationUniRule annotation

Protein-protein interaction databases

STRINGi243277.VC0902.

Structurei

Secondary structure

1
281
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi27 – 293
Beta strandi32 – 343
Helixi36 – 416
Beta strandi51 – 588
Beta strandi62 – 643
Beta strandi66 – 683
Beta strandi70 – 723
Beta strandi74 – 807
Beta strandi83 – 875
Helixi89 – 979
Turni98 – 1014
Helixi107 – 12216
Beta strandi127 – 1315
Helixi133 – 1364
Beta strandi145 – 1484
Helixi163 – 1664
Beta strandi170 – 18819
Turni189 – 1913
Beta strandi194 – 20815
Helixi210 – 2189
Turni219 – 2224
Beta strandi223 – 2253
Helixi227 – 24216
Beta strandi245 – 2539
Beta strandi259 – 26810

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3BP1X-ray1.53A/B/C/D1-281[»]
3RJ4X-ray1.75A/B1-281[»]
3RZPX-ray2.00A/B/C/D1-281[»]
3S19X-ray1.50A/B/C/D1-281[»]
3UXJX-ray1.40A/B/C/D1-281[»]
3UXVX-ray1.56A/B/C/D1-281[»]
4GHMX-ray1.62A/B1-281[»]
4IQIX-ray1.50A/B1-281[»]
ProteinModelPortaliQ9KTK0.
SMRiQ9KTK0. Positions 21-281.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KTK0.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 893Substrate binding
Regioni227 – 2282Substrate binding

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0780.
KOiK06879.
OMAiIFCDLMH.
OrthoDBiEOG6D5G0M.

Family and domain databases

HAMAPiMF_00817. QueF_type2.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KTK0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKYSDAKEL ASLTLGKKTE YANQYDPSLL QPVPRSLNRN DLHLSATLPF
60 70 80 90 100
QGCDIWTLYE LSWLNQKGLP QVAIGEVSIP ATSANLIESK SFKLYLNSYN
110 120 130 140 150
QTRFASWDEV QTRLVHDLSA CAGETVTVNV KSLNEYTAEP IVTMQGECID
160 170 180 190 200
DQDIEIANYE FDDALLQGAA QGEEVSEVLH SHLLKSNCLI TNQPDWGSVE
210 220 230 240 250
IAYHGAKMNR EALLRYLVSF REHNEFHEQC VERIFTDIMR YCQPQSLTVY
260 270 280
ARYTRRGGLD INPFRSSHQS APNHNQRMAR Q
Length:281
Mass (Da):31,969
Last modified:November 22, 2005 - v2
Checksum:i9059809F7D2D5B02
GO

Sequence cautioni

The sequence AAF94064.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE003852 Genomic DNA. Translation: AAF94064.1. Different initiation.
PIRiF82265.
RefSeqiNP_230549.2. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF94064; AAF94064; VC_0902.
GeneIDi2614193.
KEGGivch:VC0902.
PATRICi20080902. VBIVibCho83274_0859.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE003852 Genomic DNA. Translation: AAF94064.1 . Different initiation.
PIRi F82265.
RefSeqi NP_230549.2. NC_002505.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3BP1 X-ray 1.53 A/B/C/D 1-281 [» ]
3RJ4 X-ray 1.75 A/B 1-281 [» ]
3RZP X-ray 2.00 A/B/C/D 1-281 [» ]
3S19 X-ray 1.50 A/B/C/D 1-281 [» ]
3UXJ X-ray 1.40 A/B/C/D 1-281 [» ]
3UXV X-ray 1.56 A/B/C/D 1-281 [» ]
4GHM X-ray 1.62 A/B 1-281 [» ]
4IQI X-ray 1.50 A/B 1-281 [» ]
ProteinModelPortali Q9KTK0.
SMRi Q9KTK0. Positions 21-281.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243277.VC0902.

Protocols and materials databases

DNASUi 2614193.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAF94064 ; AAF94064 ; VC_0902 .
GeneIDi 2614193.
KEGGi vch:VC0902.
PATRICi 20080902. VBIVibCho83274_0859.

Phylogenomic databases

eggNOGi COG0780.
KOi K06879.
OMAi IFCDLMH.
OrthoDBi EOG6D5G0M.

Enzyme and pathway databases

UniPathwayi UPA00392 .

Miscellaneous databases

EvolutionaryTracei Q9KTK0.

Family and domain databases

HAMAPi MF_00817. QueF_type2.
InterProi IPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view ]
Pfami PF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsi TIGR03138. QueF. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.
  2. "High-resolution structure of the nitrile reductase QueF combined with molecular simulations provide insight into enzyme mechanism."
    Kim Y., Zhou M., Moy S., Morales J., Cunningham M.A., Joachimiak A.
    J. Mol. Biol. 404:127-137(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.53 ANGSTROMS) IN COMPLEX WITH GUANINE, FUNCTION, SUBUNIT, REACTION MECHANISM.
    Strain: ATCC 39315 / El Tor Inaba N16961.
  3. "Crystal structures of 7-cyano-7-deazaguanine reductase, QueF, from Vibrio cholerae."
    Center for structural genomics of infectious diseases (CSGID)
    Submitted (JAN-2013) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF WILD-TYPE AND MUTANTS ALA-188; ALA-227 AND LEU-256 IN COMPLEXES WITH NADP AND SUBSTRATE PREQ0 TRAPPED AS A COVALENT THIOIMIDE INTERMEDIATE, ACTIVE SITE CYS-188.

Entry informationi

Entry nameiQUEF_VIBCH
AccessioniPrimary (citable) accession number: Q9KTK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: October 29, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3