Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9KTK0

- QUEF_VIBCH

UniProt

Q9KTK0 - QUEF_VIBCH

Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (22 Nov 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).1 Publication

    Catalytic activityi

    7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei188 – 1881Thioimide intermediate1 PublicationUniRule annotation
    Active sitei195 – 1951Proton donorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi89 – 902NADPH
    Nucleotide bindingi256 – 2572NADPH

    GO - Molecular functioni

    1. oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Source: InterPro
    2. preQ1 synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. queuosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Queuosine biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00392.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
    Alternative name(s):
    7-cyano-7-carbaguanine reductaseUniRule annotation
    NADPH-dependent nitrile oxidoreductaseUniRule annotation
    PreQ(0) reductaseUniRule annotation
    Gene namesi
    Name:queFUniRule annotation
    Ordered Locus Names:VC_0902
    OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
    Taxonomic identifieri243277 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000000584: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 281281NADPH-dependent 7-cyano-7-deazaguanine reductasePRO_0000163062Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 PublicationUniRule annotation

    Protein-protein interaction databases

    STRINGi243277.VC0902.

    Structurei

    Secondary structure

    1
    281
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi27 – 293
    Beta strandi32 – 343
    Helixi36 – 416
    Beta strandi51 – 588
    Beta strandi62 – 643
    Beta strandi66 – 683
    Beta strandi70 – 723
    Beta strandi74 – 807
    Beta strandi83 – 875
    Helixi89 – 979
    Turni98 – 1014
    Helixi107 – 12216
    Beta strandi127 – 1315
    Helixi133 – 1364
    Beta strandi145 – 1484
    Helixi163 – 1664
    Beta strandi170 – 18819
    Turni189 – 1913
    Beta strandi194 – 20815
    Helixi210 – 2189
    Turni219 – 2224
    Beta strandi223 – 2253
    Helixi227 – 24216
    Beta strandi245 – 2539
    Beta strandi259 – 26810

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3BP1X-ray1.53A/B/C/D1-281[»]
    3RJ4X-ray1.75A/B1-281[»]
    3RZPX-ray2.00A/B/C/D1-281[»]
    3S19X-ray1.50A/B/C/D1-281[»]
    3UXJX-ray1.40A/B/C/D1-281[»]
    3UXVX-ray1.56A/B/C/D1-281[»]
    4GHMX-ray1.62A/B1-281[»]
    4IQIX-ray1.50A/B1-281[»]
    ProteinModelPortaliQ9KTK0.
    SMRiQ9KTK0. Positions 21-281.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9KTK0.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni87 – 893Substrate binding
    Regioni227 – 2282Substrate binding

    Sequence similaritiesi

    Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0780.
    KOiK06879.
    OMAiIFCDLMH.
    OrthoDBiEOG6D5G0M.

    Family and domain databases

    HAMAPiMF_00817. QueF_type2.
    InterProiIPR029500. QueF.
    IPR029139. QueF_N.
    IPR016428. QueF_type2.
    [Graphical view]
    PfamiPF14489. QueF. 1 hit.
    PF14819. QueF_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
    TIGRFAMsiTIGR03138. QueF. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9KTK0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKYSDAKEL ASLTLGKKTE YANQYDPSLL QPVPRSLNRN DLHLSATLPF    50
    QGCDIWTLYE LSWLNQKGLP QVAIGEVSIP ATSANLIESK SFKLYLNSYN 100
    QTRFASWDEV QTRLVHDLSA CAGETVTVNV KSLNEYTAEP IVTMQGECID 150
    DQDIEIANYE FDDALLQGAA QGEEVSEVLH SHLLKSNCLI TNQPDWGSVE 200
    IAYHGAKMNR EALLRYLVSF REHNEFHEQC VERIFTDIMR YCQPQSLTVY 250
    ARYTRRGGLD INPFRSSHQS APNHNQRMAR Q 281
    Length:281
    Mass (Da):31,969
    Last modified:November 22, 2005 - v2
    Checksum:i9059809F7D2D5B02
    GO

    Sequence cautioni

    The sequence AAF94064.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE003852 Genomic DNA. Translation: AAF94064.1. Different initiation.
    PIRiF82265.
    RefSeqiNP_230549.2. NC_002505.1.

    Genome annotation databases

    EnsemblBacteriaiAAF94064; AAF94064; VC_0902.
    GeneIDi2614193.
    KEGGivch:VC0902.
    PATRICi20080902. VBIVibCho83274_0859.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE003852 Genomic DNA. Translation: AAF94064.1 . Different initiation.
    PIRi F82265.
    RefSeqi NP_230549.2. NC_002505.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3BP1 X-ray 1.53 A/B/C/D 1-281 [» ]
    3RJ4 X-ray 1.75 A/B 1-281 [» ]
    3RZP X-ray 2.00 A/B/C/D 1-281 [» ]
    3S19 X-ray 1.50 A/B/C/D 1-281 [» ]
    3UXJ X-ray 1.40 A/B/C/D 1-281 [» ]
    3UXV X-ray 1.56 A/B/C/D 1-281 [» ]
    4GHM X-ray 1.62 A/B 1-281 [» ]
    4IQI X-ray 1.50 A/B 1-281 [» ]
    ProteinModelPortali Q9KTK0.
    SMRi Q9KTK0. Positions 21-281.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243277.VC0902.

    Protocols and materials databases

    DNASUi 2614193.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF94064 ; AAF94064 ; VC_0902 .
    GeneIDi 2614193.
    KEGGi vch:VC0902.
    PATRICi 20080902. VBIVibCho83274_0859.

    Phylogenomic databases

    eggNOGi COG0780.
    KOi K06879.
    OMAi IFCDLMH.
    OrthoDBi EOG6D5G0M.

    Enzyme and pathway databases

    UniPathwayi UPA00392 .

    Miscellaneous databases

    EvolutionaryTracei Q9KTK0.

    Family and domain databases

    HAMAPi MF_00817. QueF_type2.
    InterProi IPR029500. QueF.
    IPR029139. QueF_N.
    IPR016428. QueF_type2.
    [Graphical view ]
    Pfami PF14489. QueF. 1 hit.
    PF14819. QueF_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
    TIGRFAMsi TIGR03138. QueF. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39315 / El Tor Inaba N16961.
    2. "High-resolution structure of the nitrile reductase QueF combined with molecular simulations provide insight into enzyme mechanism."
      Kim Y., Zhou M., Moy S., Morales J., Cunningham M.A., Joachimiak A.
      J. Mol. Biol. 404:127-137(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.53 ANGSTROMS) IN COMPLEX WITH GUANINE, FUNCTION, SUBUNIT, REACTION MECHANISM.
      Strain: ATCC 39315 / El Tor Inaba N16961.
    3. "Crystal structures of 7-cyano-7-deazaguanine reductase, QueF, from Vibrio cholerae."
      Center for structural genomics of infectious diseases (CSGID)
      Submitted (JAN-2013) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF WILD-TYPE AND MUTANTS ALA-188; ALA-227 AND LEU-256 IN COMPLEXES WITH NADP AND SUBSTRATE PREQ0 TRAPPED AS A COVALENT THIOIMIDE INTERMEDIATE, ACTIVE SITE CYS-188.

    Entry informationi

    Entry nameiQUEF_VIBCH
    AccessioniPrimary (citable) accession number: Q9KTK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: November 22, 2005
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3