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Q9KTK0 (QUEF_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13
Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase
Gene names
Name:queF
Ordered Locus Names:VC_0902
OrganismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]
Taxonomic identifier243277 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio ›

Protein attributes

Sequence length281 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP-Rule MF_00817

Catalytic activity

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00817

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00817

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Sequence caution

The sequence AAF94064.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 281281NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00817
PRO_0000163062

Secondary structure

1 ............................................... 281
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9KTK0 [UniParc].

Last modified November 22, 2005. Version 2.
Checksum: 9059809F7D2D5B02

FASTA28131,969
        10         20         30         40         50         60 
MSKYSDAKEL ASLTLGKKTE YANQYDPSLL QPVPRSLNRN DLHLSATLPF QGCDIWTLYE 

        70         80         90        100        110        120 
LSWLNQKGLP QVAIGEVSIP ATSANLIESK SFKLYLNSYN QTRFASWDEV QTRLVHDLSA 

       130        140        150        160        170        180 
CAGETVTVNV KSLNEYTAEP IVTMQGECID DQDIEIANYE FDDALLQGAA QGEEVSEVLH 

       190        200        210        220        230        240 
SHLLKSNCLI TNQPDWGSVE IAYHGAKMNR EALLRYLVSF REHNEFHEQC VERIFTDIMR 

       250        260        270        280 
YCQPQSLTVY ARYTRRGGLD INPFRSSHQS APNHNQRMAR Q

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE003852 Genomic DNA. Translation: AAF94064.1. Different initiation.
PIRF82265.
RefSeqNP_230549.2. NC_002505.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3BP1X-ray1.53A/B/C/D1-281[»]
3RJ4X-ray1.75A/B1-281[»]
3RZPX-ray2.00A/B/C/D1-281[»]
3S19X-ray1.50A/B/C/D1-281[»]
3UXJX-ray1.40A/B/C/D1-281[»]
3UXVX-ray1.56A/B/C/D1-281[»]
4GHMX-ray1.62A/B1-281[»]
4IQIX-ray1.50A/B1-281[»]
ProteinModelPortalQ9KTK0.
SMRQ9KTK0. Positions 21-281.
ModBaseSearch...

Protein-protein interaction databases

STRING243277.VC0902.

Protocols and materials databases

DNASU2614193.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF94064; AAF94064; VC_0902.
GeneID2614193.
KEGGvch:VC0902.
PATRIC20080902. VBIVibCho83274_0859.

Phylogenomic databases

eggNOGCOG0780.
KOK06879.
OMAERIFCDL.
ProtClustDBPRK11792.

Enzyme and pathway databases

UniPathwayUPA00392.

Family and domain databases

HAMAPMF_00817. QueF_type2.
InterProIPR016428. CN_OxRdtase_NADPH-dep_YqcD.
IPR020602. GTP_CycHdrlase_I/CN_OxRdtase.
[Graphical view]
PfamPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
PIRSFPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsTIGR03138. QueF. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ9KTK0.

Entry information

Entry nameQUEF_VIBCH
AccessionPrimary (citable) accession number: Q9KTK0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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