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Q9KTG1 (GLYA1_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase 1
Short name=SHMT 1
Short name=Serine methylase 1
EC=2.1.2.1
Gene names
Name:glyA1
Ordered Locus Names:VC_0941
OrganismVibrio cholerae
Taxonomic identifier666 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence caution

The sequence AAF94103.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 416416Pyridoxal-phosphate-dependent serine hydroxymethyltransferase 1
PRO_0000113690

Regions

Region125 – 1273Substrate binding By similarity

Sites

Binding site351Pyridoxal phosphate By similarity
Binding site551Pyridoxal phosphate By similarity
Binding site571Substrate By similarity
Binding site641Substrate binding By similarity
Binding site651Pyridoxal phosphate By similarity
Binding site991Pyridoxal phosphate By similarity
Binding site1211Substrate By similarity
Binding site1751Pyridoxal phosphate By similarity
Binding site2031Pyridoxal phosphate By similarity
Binding site2281Pyridoxal phosphate By similarity
Binding site2351Pyridoxal phosphate By similarity
Binding site2621Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3621Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2291N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KTG1 [UniParc].

Last modified March 27, 2002. Version 2.
Checksum: CDAEE0AAB4C2BA6B

FASTA41645,409
        10         20         30         40         50         60 
MLKRDMNIAD YDPELYAAIQ EETLRQEEHI ELIASENYTS PRVMQAQGSQ LTNKYAEGYP 

        70         80         90        100        110        120 
GKRYYGGCEY VDKAEALAID RACQLFGCEY ANVQPHSGSQ ANSAVYMALL NPGDTVLGMS 

       130        140        150        160        170        180 
LAHGGHLTHG SPVNFSGKHY NVIPYGIDEA GQINYDEMEA LALEHKPKMI IGGFSAYSQI 

       190        200        210        220        230        240 
VDWKRMREIA DKVGAYLFVD MAHVAGLIAA GVYPSPVPFA HVVTTTTHKT LAGPRGGLIL 

       250        260        270        280        290        300 
SNAGEEMYKK LNSAVFPGGQ GGPLMHVIAA KAVAFKEAME PEFKEYQARV VKNAKAMVAQ 

       310        320        330        340        350        360 
FQERGYKIVS NSTENHLFLV DLIDKNITGK EADAALGAAN ITVNKNSVPN DPRSPFVTSG 

       370        380        390        400        410 
IRVGTPAITR RGFTEQDAKD LANWMCDVLD NINDQGVIEA TKQKVLAICQ RLPVYA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE003852 Genomic DNA. Translation: AAF94103.1. Different initiation.
PIRH82258.
RefSeqNP_230588.2. NC_002505.1.

3D structure databases

ProteinModelPortalQ9KTG1.
SMRQ9KTG1. Positions 1-416.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2614161.
GenomeReviewsGene locus VC_0941 in contig AE003852_GR.
KEGGvch:VC0941.
PATRIC20080986. VBIVibCho83274_0897.
TIGRVC_0941.

Phylogenomic databases

HOGENOMHBG301263.
OMAFSASYEM.
PhylomeDBQ9KTG1.
ProtClustDBPRK00011.

Family and domain databases

HAMAPMF_00051. SHMT.
[Tree]
InterProIPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00600.
PANTHERPTHR11680. Gly_HO-Metrfase. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA1_VIBCH
AccessionPrimary (citable) accession number: Q9KTG1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: January 25, 2012
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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