Reviewed, UniProtKB/Swiss-Prot Q9KTG1 (GLYA1_VIBCH)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 1
      Short name=Serine methylase 1
      Short name=SHMT 1
    EC=2.1.2.1

Gene names

Name:	glyA1
Ordered Locus Names:	VC_0941

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity. HAMAP MF_00051
Subcellular location	Cytoplasm By similarity HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Serine hydroxymethyltransferase 1 HAMAP MF_00051		PRO_0000113690
Amino acid modifications
	Modified residue	229	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KTG1-1 [UniParc]. Last modified March 27, 2002. Version 2. Checksum: CDAEE0AAB4C2BA6B	FASTA	416	45,409
	10 20 30 40 50 60 MLKRDMNIAD YDPELYAAIQ EETLRQEEHI ELIASENYTS PRVMQAQGSQ LTNKYAEGYP 70 80 90 100 110 120 GKRYYGGCEY VDKAEALAID RACQLFGCEY ANVQPHSGSQ ANSAVYMALL NPGDTVLGMS 130 140 150 160 170 180 LAHGGHLTHG SPVNFSGKHY NVIPYGIDEA GQINYDEMEA LALEHKPKMI IGGFSAYSQI 190 200 210 220 230 240 VDWKRMREIA DKVGAYLFVD MAHVAGLIAA GVYPSPVPFA HVVTTTTHKT LAGPRGGLIL 250 260 270 280 290 300 SNAGEEMYKK LNSAVFPGGQ GGPLMHVIAA KAVAFKEAME PEFKEYQARV VKNAKAMVAQ 310 320 330 340 350 360 FQERGYKIVS NSTENHLFLV DLIDKNITGK EADAALGAAN ITVNKNSVPN DPRSPFVTSG 370 380 390 400 410 IRVGTPAITR RGFTEQDAKD LANWMCDVLD NINDQGVIEA TKQKVLAICQ RLPVYA

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF94103.1. Different initiation.
PIR	H82258.
RefSeq	NP_230588.2.
3D structure databases
SMR	Q9KTG1. Positions 1-416.
ModBase	Search...
Genome annotation databases
GeneID	2614161.
GenomeReviews	Gene locus VC_0941 in contig AE003852_GR.
KEGG	vch:VC0941.
TIGR	VC_0941.
Phylogenomic databases
HOGENOM	HBG301263.
Enzyme and pathway databases
BRENDA	2.1.2.1. 19019.
Family and domain databases
HAMAP	MF_00051_B. SHMT_B. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA1_VIBCH

Accession

Primary (citable) accession number: Q9KTG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	March 27, 2002
Last modified:	January 19, 2010
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)