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Q9KTF9 (LIPA_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase

EC=2.8.1.8
Alternative name(s):
Lip-syn
Short name=LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene names
Name:lipA
Ordered Locus Names:VC_0943
OrganismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]
Taxonomic identifier243277 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00206.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 321321Lipoyl synthase HAMAP-Rule MF_00206
PRO_0000102377

Sites

Metal binding681Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding731Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding791Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding941Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding981Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1011Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KTF9 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 97D3D41508A0695E

FASTA32136,454
        10         20         30         40         50         60 
MSKPIQMERG VKYRDADKMA LIPIKNMPTE QKEVLRKPEW MKIKLPADSQ RIQDIKAAMR 

        70         80         90        100        110        120 
KNNLHSVCEE ASCPNLAECF NHGTATFMIL GAICTRRCPF CDVAHGRPNA PEAEEPKKLA 

       130        140        150        160        170        180 
QTIHDMKLKY VVITSVDRDD LRDGGAQHFA DCNREIRALN PHIKIETLVP DFRGRMEVAL 

       190        200        210        220        230        240 
EALKDNPPDV FNHNLETAPR LYRKVRPGAN YKWSLELLRQ FKEQHPHVPT KSGLMMGLGE 

       250        260        270        280        290        300 
TKEEIVEVLK DLRAHGVTML TLGQYLAPSR HHLPVERYVP PAEFDELKEV ALELGFTHAA 

       310        320 
CGPFVRSSYH ADLQAKGLEV K 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE003852 Genomic DNA. Translation: AAF94105.1.
PIRB82259.
RefSeqNP_230590.1. NC_002505.1.

3D structure databases

ProteinModelPortalQ9KTF9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243277.VC0943.

Protocols and materials databases

DNASU2614163.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF94105; AAF94105; VC_0943.
GeneID2614163.
KEGGvch:VC0943.
PATRIC20080990. VBIVibCho83274_0899.

Phylogenomic databases

eggNOGCOG0320.
KOK03644.
OMAHPHIPTK.
OrthoDBEOG6038ZS.

Enzyme and pathway databases

BioCycVCHO:VC0943-MONOMER.
UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLIPA_VIBCH
AccessionPrimary (citable) accession number: Q9KTF9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways