Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei621 – 6211ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: TIGR

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:VC_0956
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584 Componenti: Chromosome 1

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 858858Leucine--tRNA ligasePRO_0000152113Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243277.VC0956.

Structurei

3D structure databases

ProteinModelPortaliQ9KTE6.
SMRiQ9KTE6. Positions 228-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi618 – 6225"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KTE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEQYNPQDI EHKVQQHWDN NKTFVVSEDP NKEKFYCLSM FPYPSGRLHM
60 70 80 90 100
GHVRNYTIGD VVSRFQRLQG KNVLQPIGWD AFGLPAENAA VKNNTAPAPW
110 120 130 140 150
TYENIEYMKN QLKLLGFGYD WNREFATCRP EYYRWEQEFF TKLFAKGLVY
160 170 180 190 200
KKTSSVNWCP NDQTVLANEQ VEDGCCWRCD TPVEQKEIPQ WFIKITAYAQ
210 220 230 240 250
ELLDDLDNLD GWPEMVKTMQ RNWIGRSEGV ELKFAVKGEN TDLEVYTTRP
260 270 280 290 300
DTLMGVTYVG IAAGHPLATK AAANNPALAA FIDECKNTKV AEAEIATMEK
310 320 330 340 350
KGMATGLTAI HPLNGREVPI YIANFVLMDY GTGAVMAVPA HDQRDFEFAT
360 370 380 390 400
KYGLDIIPVI KPADGSELDV SEAAYTEKGV LFASGEFDGL DFQAAFNAIA
410 420 430 440 450
AKLEAEGKGK KTVNFRLRDW GVSRQRYWGA PIPMVTTEDG QVHPVPADQL
460 470 480 490 500
PVILPEDVVM DGVTSPIKAD KEWAKTTFNG EPALRETDTF DTFMESSWYY
510 520 530 540 550
ARYCSPQADD ILDPEKANYW LPVDQYIGGI EHACMHLLYS RFFHKLLRDA
560 570 580 590 600
GYVKSDEPFK KLLCQGMVLA DAFYYTNDKG GKEWVSPTEV KVERDGKGRI
610 620 630 640 650
VSAVDATGRQ VEHSGMIKMS KSKNNGIDPQ EMVDKYGADT VRLFMMFASP
660 670 680 690 700
ADMTLEWQES GVEGANRFLR RVWKLVREHT ELGQAPALDA SALNADQKAL
710 720 730 740 750
RRDVHKTIAK VTDDVARRQT FNTAIAAIME LMNKLTKAPM TEAQDRAILD
760 770 780 790 800
EALKAITLML YPITPHICFE MWVALGQSNI DTASWPTYDE AALVEDEKLI
810 820 830 840 850
VLQVNGKLRG KLTVAADATQ QQVEALGMQD ENVQKFIDGL TVRKVIYVPG

KLLNIVAN
Length:858
Mass (Da):96,621
Last modified:June 1, 2001 - v2
Checksum:iA4D59A2E1C9F1292
GO

Sequence cautioni

The sequence AAF94118.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94118.1. Different initiation.
PIRiG82260.
RefSeqiNP_230603.2. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF94118; AAF94118; VC_0956.
GeneIDi2614209.
KEGGivch:VC0956.
PATRICi20081012. VBIVibCho83274_0910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94118.1. Different initiation.
PIRiG82260.
RefSeqiNP_230603.2. NC_002505.1.

3D structure databases

ProteinModelPortaliQ9KTE6.
SMRiQ9KTE6. Positions 228-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0956.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF94118; AAF94118; VC_0956.
GeneIDi2614209.
KEGGivch:VC0956.
PATRICi20081012. VBIVibCho83274_0910.

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiSYL_VIBCH
AccessioniPrimary (citable) accession number: Q9KTE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: January 7, 2015
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.