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Protein

Phosphoenolpyruvate-protein phosphotransferase

Gene

VC_0965

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).UniRule annotation

Catalytic activityi

Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei189 – 1891Tele-phosphohistidine intermediateUniRule annotation
Binding sitei296 – 2961SubstrateUniRule annotation
Binding sitei332 – 3321SubstrateUniRule annotation
Metal bindingi431 – 4311MagnesiumUniRule annotation
Binding sitei431 – 4311SubstrateUniRule annotation
Binding sitei452 – 4521Substrate; via carbonyl oxygenUniRule annotation
Binding sitei453 – 4531Substrate; via amide nitrogenUniRule annotation
Binding sitei454 – 4541SubstrateUniRule annotation
Metal bindingi455 – 4551MagnesiumUniRule annotation
Binding sitei455 – 4551Substrate; via amide nitrogenUniRule annotation
Active sitei502 – 5021Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • carbohydrate transmembrane transport Source: GOC
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

Phosphotransferase systemUniRule annotation, Sugar transportUniRule annotation, Transport

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation, PyruvateImported

Enzyme and pathway databases

BioCyciVCHO:VC0965-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate-protein phosphotransferaseUniRule annotation (EC:2.7.3.9UniRule annotation)
Alternative name(s):
Phosphotransferase system, enzyme IUniRule annotation
Gene namesi
Ordered Locus Names:VC_0965Imported
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)Imported
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi243277.VC0965.

Structurei

3D structure databases

ProteinModelPortaliQ9KTD7.
SMRiQ9KTD7. Positions 2-572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 126123PEP-utilisers_NInterPro annotationAdd
BLAST
Domaini152 – 22271PEP-utilizersInterPro annotationAdd
BLAST
Domaini250 – 541292PEP-utilizers_CInterPro annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili392 – 41928Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the PEP-utilizing enzyme family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiENOG4105BZ3. Bacteria.
COG1080. LUCA.
KOiK08483.
OMAiDYVLGFA.
OrthoDBiEOG657JBQ.

Family and domain databases

Gene3Di1.10.274.10. 1 hit.
3.20.20.60. 1 hit.
3.50.30.10. 1 hit.
InterProiIPR008279. PEP-util_enz_mobile_dom.
IPR018274. PEP_util_AS.
IPR000121. PEP_util_C.
IPR023151. PEP_util_CS.
IPR024692. PTS_EI.
IPR006318. PTS_EI-like.
IPR008731. PTS_EIN.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF05524. PEP-utilisers_N. 1 hit.
PF00391. PEP-utilizers. 1 hit.
PF02896. PEP-utilizers_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000732. PTS_enzyme_I. 1 hit.
SUPFAMiSSF47831. SSF47831. 1 hit.
SSF51621. SSF51621. 1 hit.
SSF52009. SSF52009. 1 hit.
TIGRFAMsiTIGR01417. PTS_I_fam. 1 hit.
PROSITEiPS00742. PEP_ENZYMES_2. 1 hit.
PS00370. PEP_ENZYMES_PHOS_SITE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KTD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISGILASPG IAIGKALLLQ EDEIVLNTNT ISDDQVEAEV ERFYTARDKS
60 70 80 90 100
SAQLEVIKQK ALETFGEEKE AIFEGHIMLL EDEELEEEIL ALIKKEKMSA
110 120 130 140 150
DNAIHTVIEE QATALESLDD EYLKERATDI RDIGTRFVKN ALGMHIVSLS
160 170 180 190 200
EIDQEVVLVA YDLTPSETAQ INLNYVLGFA CDIGGRTSHT SIMARSLELP
210 220 230 240 250
AIVGTNDITK KVKNGDTLIL DAMNNKIIVN PTQAQIEEAK AVKAAFLAEK
260 270 280 290 300
EELAKLKDLP AETLDGHRVE VCGNIGTVKD CDGIIRNGGE GVGLYRTEFL
310 320 330 340 350
FMDRDALPTE EEQYQAYKEV AEAMNGQAVI IRTMDIGGDK DLPYMDLPKE
360 370 380 390 400
MNPFLGWRAV RISLDRREIL RDQLRGILRA SAHGKLRVMF PMIISVEEIR
410 420 430 440 450
ELKNAIEEYK AELRTEGHAF DENIEIGVMV ETPAAAAIAH HLAKEVSFFS
460 470 480 490 500
IGTNDLTQYT LAVDRGNEMI SHLYNPLSPA VLTVIKQVID ASHAEGKWTG
510 520 530 540 550
MCGELAGDER ATLLLLGMGL DEFSMSGISI PKVKKVIRNA NYAEIKAMAE
560 570
EALALPTAAE IEACVDKFIA AKA
Length:573
Mass (Da):63,132
Last modified:October 1, 2000 - v1
Checksum:iDB0E790F8BB58D72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94127.1.
PIRiH82257.
RefSeqiNP_230612.1. NC_002505.1.
WP_000623159.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF94127; AAF94127; VC_0965.
GeneIDi2614218.
KEGGivch:VC0965.
PATRICi20081030. VBIVibCho83274_0919.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94127.1.
PIRiH82257.
RefSeqiNP_230612.1. NC_002505.1.
WP_000623159.1. NC_002505.1.

3D structure databases

ProteinModelPortaliQ9KTD7.
SMRiQ9KTD7. Positions 2-572.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0965.

Protocols and materials databases

DNASUi2614218.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF94127; AAF94127; VC_0965.
GeneIDi2614218.
KEGGivch:VC0965.
PATRICi20081030. VBIVibCho83274_0919.

Phylogenomic databases

eggNOGiENOG4105BZ3. Bacteria.
COG1080. LUCA.
KOiK08483.
OMAiDYVLGFA.
OrthoDBiEOG657JBQ.

Enzyme and pathway databases

BioCyciVCHO:VC0965-MONOMER.

Family and domain databases

Gene3Di1.10.274.10. 1 hit.
3.20.20.60. 1 hit.
3.50.30.10. 1 hit.
InterProiIPR008279. PEP-util_enz_mobile_dom.
IPR018274. PEP_util_AS.
IPR000121. PEP_util_C.
IPR023151. PEP_util_CS.
IPR024692. PTS_EI.
IPR006318. PTS_EI-like.
IPR008731. PTS_EIN.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF05524. PEP-utilisers_N. 1 hit.
PF00391. PEP-utilizers. 1 hit.
PF02896. PEP-utilizers_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000732. PTS_enzyme_I. 1 hit.
SUPFAMiSSF47831. SSF47831. 1 hit.
SSF51621. SSF51621. 1 hit.
SSF52009. SSF52009. 1 hit.
TIGRFAMsiTIGR01417. PTS_I_fam. 1 hit.
PROSITEiPS00742. PEP_ENZYMES_2. 1 hit.
PS00370. PEP_ENZYMES_PHOS_SITE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961Imported.

Entry informationi

Entry nameiQ9KTD7_VIBCH
AccessioniPrimary (citable) accession number: Q9KTD7
Entry historyi
Integrated into UniProtKB/TrEMBL: October 1, 2000
Last sequence update: October 1, 2000
Last modified: May 11, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.