Q9KT93 (LGUL_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable lactoylglutathione lyase
EC=4.4.1.5

Alternative name(s):
Aldoketomutase
Glyoxalase I
Short name=Glx I
Ketone-aldehyde mutase
Methylglyoxalase
S-D-lactoylglutathione methylglyoxal lyase

Gene names

Name:	gloA
Ordered Locus Names:	VC_1010

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1 › Vibrio cholerae O1 biovar El Tor

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione By similarity.
Catalytic activity	(R)-S-lactoylglutathione = glutathione + methylglyoxal.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Sequence similarities	Belongs to the glyoxalase I family.
Sequence caution	The sequence AAF94171.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Metal-binding Nickel
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutathione metabolic process Inferred from sequence or structural similarity Ref.1. Source: TIGR
Molecular_function	lactoylglutathione lyase activity Inferred from sequence or structural similarity Ref.1. Source: TIGR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 138	138	Probable lactoylglutathione lyase		PRO_0000168097
Sites
	Metal binding	8	1	Nickel By similarity
	Metal binding	59	1	Nickel By similarity
	Metal binding	77	1	Nickel By similarity
	Metal binding	125	1	Nickel By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KT93 [UniParc]. Last modified March 20, 2007. Version 2. Checksum: 21427CF9AA2E51BD	FASTA	138	15,176
	10 20 30 40 50 60 MSNHRILHTM LRVGDLDKSI EFYTQVMGMS LLRKNENTEY KYTLAFLGYG DESQGAVIEL 70 80 90 100 110 120 TYNWGVADYE KGNAYGHIAI GVDDIYATCD TIKAAGGIVT REPGPVKGGT THIAFVKDPD 130 GYMIELIQNK QAHAGLEG

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF94171.1. Different initiation.
PIR	H82251.
RefSeq	NP_230656.2. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KT93.
SMR	Q9KT93. Positions 5-128.
ModBase	Search...
Protein-protein interaction databases
STRING	243277.VC1010.
Protocols and materials databases
DNASU	2614263.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF94171; AAF94171; VC_1010.
GeneID	2614263.
KEGG	vch:VC1010.
PATRIC	20081120. VBIVibCho83274_0964.
Phylogenomic databases
eggNOG	COG0346.
KO	K01759.
OMA	VREPGPM.
ProtClustDB	CLSK2484291.
Enzyme and pathway databases
UniPathway	UPA00619; UER00675.
Family and domain databases
InterPro	IPR004360. Glyas_Fos-R_dOase_dom. IPR004361. Glyoxalase_1. IPR018146. Glyoxalase_1_CS. [Graphical view]
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00068. glyox_I. 1 hit.
PROSITE	PS00934. GLYOXALASE_I_1. 1 hit. PS00935. GLYOXALASE_I_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LGUL_VIBCH

Accession

Primary (citable) accession number: Q9KT93

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	March 20, 2007
Last modified:	May 29, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program