Reviewed, UniProtKB/Swiss-Prot Q9KT93 (LGUL_VIBCH)

Names and origin

Protein names

Recommended name:
    Probable lactoylglutathione lyase
    EC=4.4.1.5
Alternative name(s):
    Methylglyoxalase
    Aldoketomutase
    Glyoxalase I
      Short name=Glx I
    Ketone-aldehyde mutase
    S-D-lactoylglutathione methylglyoxal lyase

Gene names

Name:	gloA
Ordered Locus Names:	VC_1010

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione By similarity.
Catalytic activity	(R)-S-lactoylglutathione = glutathione + methylglyoxal.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Sequence similarities	Belongs to the glyoxalase I family.

Ontologies

Keywords
Ligand	Metal-binding Nickel
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	lactoylglutathione lyase activity Inferred from electronic annotation. Source: EC nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 138	138	Probable lactoylglutathione lyase		PRO_0000168097
Sites
	Metal binding	8	1	Nickel By similarity
	Metal binding	59	1	Nickel By similarity
	Metal binding	77	1	Nickel By similarity
	Metal binding	125	1	Nickel By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KT93-1 [UniParc]. Last modified March 20, 2007. Version 2. Checksum: 21427CF9AA2E51BD	FASTA	138	15,176
	10 20 30 40 50 60 MSNHRILHTM LRVGDLDKSI EFYTQVMGMS LLRKNENTEY KYTLAFLGYG DESQGAVIEL 70 80 90 100 110 120 TYNWGVADYE KGNAYGHIAI GVDDIYATCD TIKAAGGIVT REPGPVKGGT THIAFVKDPD 130 GYMIELIQNK QAHAGLEG

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE003852 Genomic DNA. Translation: AAF94171.1. Different initiation.
PIR	H82251.
RefSeq	NP_230656.1.
3D structure databases
HSSP	HSSP built from PDB template 1F9Z based on UniProtKB Q59384.
ModBase	Search...
Genome annotation databases
GeneID	2614263.
GenomeReviews	Gene locus VC_1010 in contig AE003852_GR.
KEGG	vch:VC1010.
TIGR	VC_1010.
Phylogenomic databases
HOGENOM	Q9KT93.
Enzyme and pathway databases
BRENDA	4.4.1.5. 19019.
Family and domain databases
InterPro	IPR004360. Glyas_bleo-R_dOase. IPR004361. Glyoxalase_1. IPR018146. Glyoxalase_1_CS. [Graphical view]
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
ProDom	PD002334. Gly_diox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00068. glyox_I. 1 hit.
PROSITE	PS00934. GLYOXALASE_I_1. 1 hit. PS00935. GLYOXALASE_I_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LGUL_VIBCH

Accession

Primary (citable) accession number: Q9KT93

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	March 20, 2007
Last modified:	September 22, 2009
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)