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Protein

Probable lactoylglutathione lyase

Gene

gloA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.By similarity

Catalytic activityi

(R)-S-lactoylglutathione = glutathione + methylglyoxal.

Cofactori

Ni2+By similarityNote: Binds 1 nickel ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Nickel; via tele nitrogenBy similarity
Binding sitei12 – 121SubstrateBy similarity
Metal bindingi59 – 591NickelBy similarity
Binding sitei63 – 631SubstrateBy similarity
Metal bindingi77 – 771Nickel; via tele nitrogenBy similarity
Binding sitei77 – 771SubstrateBy similarity
Active sitei125 – 1251Proton donor/acceptorBy similarity
Metal bindingi125 – 1251NickelBy similarity

GO - Molecular functioni

  • lactoylglutathione lyase activity Source: TIGR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • glutathione metabolic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00619; UER00675.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable lactoylglutathione lyase (EC:4.4.1.5)
Alternative name(s):
Aldoketomutase
Glyoxalase I
Short name:
Glx I
Ketone-aldehyde mutase
Methylglyoxalase
S-D-lactoylglutathione methylglyoxal lyase
Gene namesi
Name:gloA
Ordered Locus Names:VC_1010
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 138138Probable lactoylglutathione lyasePRO_0000168097Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243277.VC1010.

Structurei

3D structure databases

ProteinModelPortaliQ9KT93.
SMRiQ9KT93. Positions 5-128.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glyoxalase I family.Curated

Phylogenomic databases

eggNOGiCOG0346.
KOiK01759.
OMAiTHNWDTP.
OrthoDBiEOG6DVJXX.

Family and domain databases

Gene3Di3.10.180.10. 1 hit.
InterProiIPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR004361. Glyoxalase_1.
IPR018146. Glyoxalase_1_CS.
[Graphical view]
PfamiPF00903. Glyoxalase. 1 hit.
[Graphical view]
SUPFAMiSSF54593. SSF54593. 1 hit.
TIGRFAMsiTIGR00068. glyox_I. 1 hit.
PROSITEiPS00934. GLYOXALASE_I_1. 1 hit.
PS00935. GLYOXALASE_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KT93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNHRILHTM LRVGDLDKSI EFYTQVMGMS LLRKNENTEY KYTLAFLGYG
60 70 80 90 100
DESQGAVIEL TYNWGVADYE KGNAYGHIAI GVDDIYATCD TIKAAGGIVT
110 120 130
REPGPVKGGT THIAFVKDPD GYMIELIQNK QAHAGLEG
Length:138
Mass (Da):15,176
Last modified:March 20, 2007 - v2
Checksum:i21427CF9AA2E51BD
GO

Sequence cautioni

The sequence AAF94171.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94171.1. Different initiation.
PIRiH82251.
RefSeqiNP_230656.2. NC_002505.1.
WP_000064966.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF94171; AAF94171; VC_1010.
GeneIDi2614263.
KEGGivch:VC1010.
PATRICi20081120. VBIVibCho83274_0964.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94171.1. Different initiation.
PIRiH82251.
RefSeqiNP_230656.2. NC_002505.1.
WP_000064966.1. NC_002505.1.

3D structure databases

ProteinModelPortaliQ9KT93.
SMRiQ9KT93. Positions 5-128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC1010.

Protocols and materials databases

DNASUi2614263.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF94171; AAF94171; VC_1010.
GeneIDi2614263.
KEGGivch:VC1010.
PATRICi20081120. VBIVibCho83274_0964.

Phylogenomic databases

eggNOGiCOG0346.
KOiK01759.
OMAiTHNWDTP.
OrthoDBiEOG6DVJXX.

Enzyme and pathway databases

UniPathwayiUPA00619; UER00675.

Miscellaneous databases

PROiQ9KT93.

Family and domain databases

Gene3Di3.10.180.10. 1 hit.
InterProiIPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR004361. Glyoxalase_1.
IPR018146. Glyoxalase_1_CS.
[Graphical view]
PfamiPF00903. Glyoxalase. 1 hit.
[Graphical view]
SUPFAMiSSF54593. SSF54593. 1 hit.
TIGRFAMsiTIGR00068. glyox_I. 1 hit.
PROSITEiPS00934. GLYOXALASE_I_1. 1 hit.
PS00935. GLYOXALASE_I_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiLGUL_VIBCH
AccessioniPrimary (citable) accession number: Q9KT93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: March 20, 2007
Last modified: May 27, 2015
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.