Regulatory protein LuxO - Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

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Q9KT84 (LUXO_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 82. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Regulatory protein LuxO

Gene names

Name:	luxO
Ordered Locus Names:	VC_1021

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio ›

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Involved in the regulation of different processes depending on the cell density. Acts together with sigma-54 to repress, perhaps indirectly, some genes.

Miscellaneous

In strain El Tor C6706, at low cell density, LuxO acts indirectly on virulence gene expression by repressing hapR This leads to the expression of virulence factors. In strain El Tor N16961, the hapR gene is inactive due to a natural frameshift mutation.

Sequence similarities

Contains 1 response regulatory domain.

Contains 1 sigma-54 factor interaction domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro phosphorelay response regulator activity Inferred from sequence or structural similarity Ref.1. Source: TIGR sequence-specific DNA binding transcription factor activity Inferred from sequence or structural similarity Ref.1. Source: TIGR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 455

455

Regulatory protein LuxO

PRO_0000081115

Regions

Domain

1 – 112

112

Response regulatory

Domain

132 – 361

230

Sigma-54 factor interaction

Nucleotide binding

160 – 167

ATP By similarity

Nucleotide binding

223 – 232

ATP By similarity

Amino acid modifications

Modified residue

4-aspartylphosphate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9KT84 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 0D2D2D02B95EF872
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FASTA

455

50,839

        10         20         30         40         50         60 
MVEDTASVAA LYRSYLTPLD IDINIVGTGR DAIESIGRRE PDLILLDLRL PDMTGMDVLY 

        70         80         90        100        110        120 
AVKEKSPDVP IVFMTAHGSI DTAVEAMRHG AQDFLIKPCE ADRLRVTVNN AIRKASKLKN 

       130        140        150        160        170        180 
DVDNKNQNYQ GFIGSSQTMQ AVYRTIDSAA SSKASIFITG ESGTGKEVCA EAIHAASKRG 

       190        200        210        220        230        240 
DKPFIAINCA AIPKDLIESE LFGHVKGAFT GAATERQGAA EAADGGTLFL DELCEMDLDL 

       250        260        270        280        290        300 
QTKLLRFIQT GTFQKVGSSK MKSVDVRFVC ATNRDPWKEV QEGRFREDLY YRLYVIPLHL 

       310        320        330        340        350        360 
PPLRARGDDV IEIAYSLLGF MSKEEGKDFV RLSAEVVERF RQYEWPGNVR QLQNVLRNVV 

       370        380        390        400        410        420 
VLNEGREITL DMLPPPLNQM SAPINRALPL AHENKVSVHE IFPLWMTEKQ AIEQAIEACD 

       430        440        450 
GNIPRAATYL DVSPSTIYRK LQTWNEKVKE KEKER

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.
[2]	"Identification of multiple sigma54-dependent transcriptional activators in Vibrio cholerae." Klose K.E., Novik V., Mekalanos J.J. J. Bacteriol. 180:5256-5259(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SIGMA-54.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF94180.1.
PIR	C82250.
RefSeq	NP_230666.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KT84.
ModBase	Search...
Protein-protein interaction databases
STRING	243277.VC1021.
Protocols and materials databases
DNASU	2614291.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF94180; AAF94180; VC_1021.
GeneID	2614291.
KEGG	vch:VC1021.
PATRIC	20081144. VBIVibCho83274_0975.
Phylogenomic databases
eggNOG	COG2204.
KO	K10912.
OMA	HEEGREF.
ProtClustDB	CLSK874182.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR011006. CheY-like_superfamily. IPR009057. Homeodomain-like. IPR002197. HTH_Fis. IPR001789. Sig_transdc_resp-reg_receiver. IPR002078. Sigma_54_int. IPR025943. Sigma_54_int_dom_ATP-bd_2. IPR025944. Sigma_54_int_dom_CS. [Graphical view]
Pfam	PF02954. HTH_8. 1 hit. PF00072. Response_reg. 1 hit. PF00158. Sigma54_activat. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52172. CheY_like. 1 hit. SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS50110. RESPONSE_REGULATORY. 1 hit. PS00675. SIGMA54_INTERACT_1. False negative. PS00676. SIGMA54_INTERACT_2. 1 hit. PS00688. SIGMA54_INTERACT_3. 1 hit. PS50045. SIGMA54_INTERACT_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXO_VIBCH

Accession

Primary (citable) accession number: Q9KT84

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents