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Protein

3-ketoacyl-CoA thiolase

Gene

fadI

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed.UniRule annotation

Catalytic activityi

Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98Acyl-thioester intermediateUniRule annotation1
Active sitei391Proton acceptorUniRule annotation1
Active sitei421Proton acceptorUniRule annotation1

GO - Molecular functioni

  • acetyl-CoA C-acyltransferase activity Source: TIGR

GO - Biological processi

  • fatty acid beta-oxidation Source: GO_Central
  • fatty acid catabolic process Source: TIGR

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA thiolaseUniRule annotation (EC:2.3.1.16UniRule annotation)
Alternative name(s):
ACSsUniRule annotation
Acetyl-CoA acyltransferaseUniRule annotation
Acyl-CoA ligaseUniRule annotation
Beta-ketothiolaseUniRule annotation
Fatty acid oxidation complex subunit betaUniRule annotation
Gene namesi
Name:fadIUniRule annotation
Ordered Locus Names:VC_1046
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002064491 – 4353-ketoacyl-CoA thiolaseAdd BLAST435

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi243277.VC1046.

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHU. Bacteria.
COG0183. LUCA.
KOiK00632.
OMAiMTAFPEP.

Family and domain databases

CDDicd00751. thiolase. 1 hit.
Gene3Di3.40.47.10. 2 hits.
HAMAPiMF_01618. FadI. 1 hit.
InterProiView protein in InterPro
IPR012806. Ac-CoA_C-AcTrfase_FadI.
IPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
PANTHERiPTHR18919:SF113. PTHR18919:SF113. 1 hit.
PfamiView protein in Pfam
PF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
TIGR02446. FadI. 1 hit.
PROSITEiView protein in PROSITE
PS00737. THIOLASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KT59-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKQEVRTRS GERVAIVAGL RTPFARQSTE FGQVPAVDLG KMVVQEMMAR
60 70 80 90 100
TAIDPKLIEQ VVFGQVVQMP EAPNIAREIV LGTGMSINTD AYSVTRACAT
110 120 130 140 150
SFQAAVNVAE SIMAGSIDIG IAGGADSSSV LPIGVSKKLA ASLLALSKTK
160 170 180 190 200
TVGQKLKLLS NLSFKDLMPV PPAVAEYSTG LSMGQTAEQM AKSYAISRAE
210 220 230 240 250
QDALAHRSHT LAAQAWAEGK IRDEVMTAFP EPYKKWLDMD NNIRMDSKLE
260 270 280 290 300
SYAKLRPAFD RQYGSVTAAN STPLTDGAAA IMLMREGKAK ELGLEIMGYI
310 320 330 340 350
RSYAFAAIGV EKDMLMGPSY ATPIALDRAG ITLNDLTLID MHEAFAAQTL
360 370 380 390 400
ANLKMFASDK FAQEQLGRAQ AIGEVDMSKF NVLGGSLAYG HPFAATGARM
410 420 430
ITQTLRELKR RGGGLALNTA CAAGGLGAAM ILEVE
Length:435
Mass (Da):46,354
Last modified:December 6, 2005 - v2
Checksum:i600CA5A5DCBF7270
GO

Sequence cautioni

The sequence AAF94205 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94205.1. Different initiation.
PIRiE82248.
RefSeqiNP_230691.2. NC_002505.1.
WP_000517921.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF94205; AAF94205; VC_1046.
GeneIDi2614316.
KEGGivch:VC1046.
PATRICifig|243277.26.peg.998.

Similar proteinsi

Entry informationi

Entry nameiFADI_VIBCH
AccessioniPrimary (citable) accession number: Q9KT59
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: October 25, 2017
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families