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Protein

8-amino-7-oxononanoate synthase

Gene

bioF

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein].UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei23SubstrateUniRule annotation1
Binding sitei135SubstrateUniRule annotation1
Binding sitei179Pyridoxal phosphateUniRule annotation1
Binding sitei206Pyridoxal phosphateUniRule annotation1
Binding sitei232Pyridoxal phosphateUniRule annotation1
Binding sitei348SubstrateUniRule annotation1

GO - Molecular functioni

  • 8-amino-7-oxononanoate synthase activity Source: TIGR
  • pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  • biotin biosynthetic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciVCHO:VC1113-MONOMER.
UniPathwayiUPA00078.

Names & Taxonomyi

Protein namesi
Recommended name:
8-amino-7-oxononanoate synthaseUniRule annotation (EC:2.3.1.47UniRule annotation)
Short name:
AONSUniRule annotation
Alternative name(s):
7-keto-8-amino-pelargonic acid synthaseUniRule annotation
Short name:
7-KAP synthaseUniRule annotation
Short name:
KAPA synthaseUniRule annotation
8-amino-7-ketopelargonate synthaseUniRule annotation
Gene namesi
Name:bioFUniRule annotation
Ordered Locus Names:VC_1113
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003811321 – 3848-amino-7-oxononanoate synthaseAdd BLAST384

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei235N6-(pyridoxal phosphate)lysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243277.VC1113.

Structurei

3D structure databases

ProteinModelPortaliQ9KSZ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni110 – 111Pyridoxal phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107EEK. Bacteria.
COG0156. LUCA.
KOiK00652.
OMAiDELCHAS.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01693. BioF_aminotrans_2. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR004723. AONS_Archaea/Proteobacteria.
IPR022834. AONS_Proteobacteria.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00858. bioF. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KSZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLRFKSRIE QALNEREHSR LTRRLQLLDR SAQGELIQQD RTYLNFSSND
60 70 80 90 100
YLGLANDAEL VQAWQTGLAR FGAGSGASPM VTGFSSAHAE LEHELCHWLG
110 120 130 140 150
YERAVLFSSG FSANQALLFT LLEKEDLLLQ DKLNHASLME AGMLSPATMK
160 170 180 190 200
RFKHNDTEHL RQLLHEQSNS LVVTEGVFSM DGDCAPLAQI ATLVKQRAWL
210 220 230 240 250
MVDDAHGIGV LGEDGAGSCQ AAGIHPEILV VTFGKAFGLA GAAVLCDAHV
260 270 280 290 300
GDYLTQFARH HVYSTAMPPA QAHALTHAAR MIQSQSWRRE QLAELLACFD
310 320 330 340 350
EQCRNIPGFV ATQTPIKPWL LGSSDSALSA SHELKQQGIW VSAIRPPTVP
360 370 380
VGSARLRITI TAAHTQAQIR RLSEQLKHVM EKDT
Length:384
Mass (Da):42,277
Last modified:October 1, 2000 - v1
Checksum:i36400E24B4E73082
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94272.1.
PIRiA82239.
RefSeqiNP_230758.1. NC_002505.1.
WP_000175226.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF94272; AAF94272; VC_1113.
GeneIDi2614383.
KEGGivch:VC1113.
PATRICi20081318. VBIVibCho83274_1062.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94272.1.
PIRiA82239.
RefSeqiNP_230758.1. NC_002505.1.
WP_000175226.1. NC_002505.1.

3D structure databases

ProteinModelPortaliQ9KSZ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC1113.

Protocols and materials databases

DNASUi2614383.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF94272; AAF94272; VC_1113.
GeneIDi2614383.
KEGGivch:VC1113.
PATRICi20081318. VBIVibCho83274_1062.

Phylogenomic databases

eggNOGiENOG4107EEK. Bacteria.
COG0156. LUCA.
KOiK00652.
OMAiDELCHAS.

Enzyme and pathway databases

UniPathwayiUPA00078.
BioCyciVCHO:VC1113-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01693. BioF_aminotrans_2. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR004723. AONS_Archaea/Proteobacteria.
IPR022834. AONS_Proteobacteria.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00858. bioF. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBIOF_VIBCH
AccessioniPrimary (citable) accession number: Q9KSZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.