Q9KST9 (KITH_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	VC_1167

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1 › Vibrio cholerae O1 biovar El Tor

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW nucleobase-containing small molecule interconversion Inferred from sequence or structural similarity Ref.1. Source: TIGR
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from sequence or structural similarity Ref.1. Source: TIGR zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 192	192	Thymidine kinase HAMAP-Rule MF_00124		PRO_0000175043
Regions
	Nucleotide binding	9 – 16	8	ATP By similarity
	Nucleotide binding	87 – 90	4	ATP By similarity
Sites
	Active site	88	1	Proton acceptor Potential
	Metal binding	145	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity
	Metal binding	182	1	Zinc By similarity
	Metal binding	185	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KST9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: B2532D1C6CBA9E8F	FASTA	192	21,659
	10 20 30 40 50 60 MAQMYFYYSA MNAGKSTTLL QSAFNYQERG MNPLIFTAAI DDRFGVGKVS SRIGLEAEAH 70 80 90 100 110 120 LFHADTDLLH VIATLHEAEP RHCILMDECQ FLSKEQVYQL TEVVDKLDIP VLCYGLRTDF 130 140 150 160 170 180 LGELFEGSKY LLSWADKLIE LKTICHCGRK ANMVIRTDEH GNAISEGDQV AIGGNDKYVS 190 VCRQHYKEAL GR

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF94326.1.
PIR	B82232.
RefSeq	NP_230812.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KST9.
ModBase	Search...
Protein-protein interaction databases
STRING	243277.VC1167.
Protocols and materials databases
DNASU	2614600.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF94326; AAF94326; VC_1167.
GeneID	2614600.
KEGG	vch:VC1167.
PATRIC	20081426. VBIVibCho83274_1116.
Phylogenomic databases
eggNOG	COG1435.
KO	K00857.
OMA	FRYGAMN.
ProtClustDB	PRK04296.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_VIBCH

Accession

Primary (citable) accession number: Q9KST9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program