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Protein

Tryptophan biosynthesis protein TrpCF

Gene

trpCF

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the TrpF domain; the second reaction is catalyzed by the synthase, coded by the TrpC domain (By similarity).By similarity

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.

Pathway: L-tryptophan biosynthesis

This protein is involved in step 3 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 2 (trpG), Anthranilate synthase component 1 (trpE)
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. Tryptophan biosynthesis protein TrpCF (trpCF)
  4. Tryptophan biosynthesis protein TrpCF (trpCF)
  5. Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • indole-3-glycerol-phosphate synthase activity Source: TIGR
  • phosphoribosylanthranilate isomerase activity Source: TIGR

GO - Biological processi

  • tryptophan biosynthetic process Source: TIGR

Keywords - Molecular functioni

Decarboxylase, Isomerase, Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciVCHO:VC1171-MONOMER.
UniPathwayiUPA00035; UER00042.
UPA00035; UER00043.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan biosynthesis protein TrpCF
Including the following 2 domains:
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phospho-ribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:trpCF
Ordered Locus Names:VC_1171
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Tryptophan biosynthesis protein TrpCFPRO_0000154285Add
BLAST

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi243277.VC1171.

Structurei

3D structure databases

ProteinModelPortaliQ9KST5.
SMRiQ9KST5. Positions 16-469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 271271Indole-3-glycerol phosphate synthaseAdd
BLAST
Regioni272 – 469198N-(5'-phosphoribosyl)anthranilate isomeraseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the TrpC family.Curated
In the C-terminal section; belongs to the TrpF family.Curated

Phylogenomic databases

eggNOGiCOG0134.
KOiK13498.
OMAiSFILECK.
OrthoDBiEOG6WT8JX.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
HAMAPiMF_00134_B. IGPS_B.
MF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 2 hits.
PROSITEiPS00614. IGPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KST5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQLSEHIS VQTAQMAQVL VKIVNDKYQW IEERKAKQPL ASFHPLLTRS
60 70 80 90 100
ERDFYQALSG DNTVFILECK KASPSKGLIR EEFDLDYIAS VYGEYASAIS
110 120 130 140 150
VLTDEKYFQG RFEFLPQVRA QVAQPVLCKD FMVDPYQVYL ARHYQADAIL
160 170 180 190 200
LMLSVLNDDQ YRELAAVAHD LGMGVLTEVS NEQELKRAVD LQAKVIGINN
210 220 230 240 250
RNLRDLTTDL NRTKQLAPLI PQGTIIISES GIYTHQQVRD LAEFANGFLI
260 270 280 290 300
GSSLMAQSNL ELAVRKIVLG EHKVCGLTHP DDAVKAYQAG SVFGGLIFVE
310 320 330 340 350
KSKRFVDVEQ ARLTMSGAPL QYVGVFQNHP AAQVADIATK LGLFAVQLHG
360 370 380 390 400
EEDSAYVSEL RASLPESIEI WKAYGVSDAL PELLPEHIDR HLLDAKVGEQ
410 420 430 440 450
SGGTGRSFDW RLLPKHSDLM LAGGLSAENV AQAAQLGCRG LDFNSGVESA
460
PGKKDANQLQ QVFQQLRNY
Length:469
Mass (Da):52,038
Last modified:October 1, 2000 - v1
Checksum:iAAFF776A0D8EC66E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94330.1.
PIRiF82232.
RefSeqiNP_230816.1. NC_002505.1.
WP_000003291.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF94330; AAF94330; VC_1171.
GeneIDi2614604.
KEGGivch:VC1171.
PATRICi20081434. VBIVibCho83274_1120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94330.1.
PIRiF82232.
RefSeqiNP_230816.1. NC_002505.1.
WP_000003291.1. NC_002505.1.

3D structure databases

ProteinModelPortaliQ9KST5.
SMRiQ9KST5. Positions 16-469.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC1171.

Protocols and materials databases

DNASUi2614604.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF94330; AAF94330; VC_1171.
GeneIDi2614604.
KEGGivch:VC1171.
PATRICi20081434. VBIVibCho83274_1120.

Phylogenomic databases

eggNOGiCOG0134.
KOiK13498.
OMAiSFILECK.
OrthoDBiEOG6WT8JX.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00042.
UPA00035; UER00043.
BioCyciVCHO:VC1171-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
HAMAPiMF_00134_B. IGPS_B.
MF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 2 hits.
PROSITEiPS00614. IGPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiTRPC_VIBCH
AccessioniPrimary (citable) accession number: Q9KST5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 1, 2000
Last modified: May 27, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.