Q9KSS4 (TRXB_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thioredoxin reductase
Short name=TRXR
EC=1.8.1.9

Gene names

Name:	trxB
Ordered Locus Names:	VC_1182

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	removal of superoxide radicals Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Thioredoxin reductase		PRO_0000166756
Regions
	Nucleotide binding	36 – 43	8	FAD By similarity
	Nucleotide binding	286 – 295	10	FAD By similarity
Amino acid modifications
	Disulfide bond	136 ↔ 139		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KSS4 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A0D92BE6C8AAA2FA	FASTA	318	34,346
	10 20 30 40 50 60 MSNVKHSKLL ILGSGPAGYT AAVYAARANL KPVLVTGMQQ GGQLTTTTEV ENWPGDAEGL 70 80 90 100 110 120 TGPALMERMK EHAERFDTEI VFDHINSVDL SSRPFRLTGD SQEYTCDALI ISTGASAKYL 130 140 150 160 170 180 GLESEEAFKG RGVSACATCD GFFYRNQKVA VVGGGNTAVE EALYLSNIAS EVHLVHRRDS 190 200 210 220 230 240 FRSEKILIDR LMDKVANGNI VLHTHRTLDE VLGDEMGVTG VRLKDTQSDM TENLDVMGVF 250 260 270 280 290 300 IAIGHQPNSQ IFEGQLEMKN GYIVVKSGLE GNATQTSIEG VFAAGDVMDH NYRQAITSAG 310 TGCMAALDAE RYLDSQGK

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF94341.1.
PIR	C82231.
RefSeq	NP_230827.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KSS4.
SMR	Q9KSS4. Positions 5-314.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2614615.
GenomeReviews	Gene locus VC_1182 in contig AE003852_GR.
KEGG	vch:VC1182.
PATRIC	20081454. VBIVibCho83274_1130.
TIGR	VC_1182.
Phylogenomic databases
HOGENOM	HBG669726.
OMA	VMGAFIA.
PhylomeDB	Q9KSS4.
ProtClustDB	CLSK874247.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
KO	K00384.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXB_VIBCH

Accession

Primary (citable) accession number: Q9KSS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program