Reviewed,
UniProtKB/Swiss-Prot Q9KSR8 (MAO1_VIBCH)
Last modified
January 19, 2010.
Version 49.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: NAD-dependent malic enzyme Short name=NAD-ME EC=1.1.1.38 | ||||
| Gene names |
| ||||
| Organism | Vibrio cholerae [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 666 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio |
Protein attributes
| Sequence length | 562 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Catalytic activity | (S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619 |
| Cofactor | Divalent metal cations. Prefers magnesium or manganese By similarity. HAMAP MF_01619 |
| Subunit structure | Homotetramer By similarity. HAMAP MF_01619 |
| Sequence similarities | Belongs to the malic enzymes family. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Metal-binding NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activityInferred from electronic annotation. Source: HAMAP metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 562 | 562 | NAD-dependent malic enzyme HAMAP MF_01619 | PRO_0000160234 | |||||
Sites | |||||||||
| Active site | 101 | 1 | Proton donor By similarity | ||||||
| Active site | 172 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 243 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 244 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 267 | 1 | Divalent metal cation By similarity | ||||||
| Binding site | 154 | 1 | NAD By similarity | ||||||
| Binding site | 267 | 1 | NAD By similarity | ||||||
| Binding site | 415 | 1 | NAD By similarity | ||||||
| Site | 267 | 1 | Important for activity By similarity | ||||||
Sequences
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References
| [1] | "DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S.  Fraser C.M.Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE003852 Genomic DNA. Translation: AAF94347.1. Different initiation. |
| PIR | A82232. |
| RefSeq | NP_230833.2. |
3D structure databases | |
| SMR | Q9KSR8. Positions 13-562. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 2614621. |
| GenomeReviews | Gene locus VC_1188 in contig AE003852_GR. |
| KEGG | vch:VC1188. |
| TIGR | VC_1188. |
Phylogenomic databases | |
| HOGENOM | HBG289821. |
Enzyme and pathway databases | |
| BRENDA | 1.1.1.38. 19019. |
Family and domain databases | |
| HAMAP | MF_01619. NAD_malic_enz. [Tree] |
| InterPro | IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| Pfam | PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view] |
| PRINTS | PR00072. MALOXRDTASE. |
| PROSITE | PS00331. MALIC_ENZYMES. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MAO1_VIBCH | ||||||||
| Accession | Primary (citable) accession number: Q9KSR8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
