Q9KSR8 (MAO1_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD-dependent malic enzyme
Short name=NAD-ME
EC=1.1.1.38

Gene names

Name:	maeA
Ordered Locus Names:	VC_1188

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity. HAMAP MF_01619
Sequence similarities	Belongs to the malic enzymes family.
Sequence caution	The sequence AAF94347.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	NAD-dependent malic enzyme HAMAP MF_01619		PRO_0000160234
Sites
	Active site	101	1	Proton donor By similarity
	Active site	172	1	Proton acceptor By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Binding site	154	1	NAD By similarity
	Binding site	267	1	NAD By similarity
	Binding site	415	1	NAD By similarity
	Site	267	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KSR8 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 934D1EEF53832130	FASTA	562	62,153
	10 20 30 40 50 60 MNNDKRPLYI SYAGPALLST PLLNKGSAFS AEERASFNLE GLLPEATETI QEQVVRAYQQ 70 80 90 100 110 120 YRGFESDMDK HIYLRNIQDT NETLFYRLVQ NHISEMMPII YTPTVGAACE NFSNIYRRGR 130 140 150 160 170 180 GLFISYANRD RIDDLLNNAA NHNVKVIVVT DGERILGLGD QGIGGMGIPI GKLSLYTACG 190 200 210 220 230 240 GISPAYTLPV VLDVGTNNPQ RLADPMYMGW RHPRITGPDY DNFVEEFMQA VQRRWPDALI 250 260 270 280 290 300 QFEDFAQKNA MPLLERYKNR VCCFNDDIQG TAAVTVGSLL AACKAAGSQL SQQRITFLGA 310 320 330 340 350 360 GSAGCGIAEA IIAQMVSEGI SDEQARSQVY MVDRWGLLEE GMPNLLDFQQ RLVQKKANTQ 370 380 390 400 410 420 HWTTENNGYS LHDVIRNAKP TVLVGVSGAP GLFSEEIIKE MHQHCPRPIV FPLSNPTSRV 430 440 450 460 470 480 EALPSDIIRW TNGEALVATG SPFDPVLHEG KTYPIVQCNN SYIFPGIGLG VLAANARRVT 490 500 510 520 530 540 DEMLMESSRA LASCSPLAIN GHGPLLPPLE SIHSVSKKIA FAVAKKAIEQ GVAPEVTDEA 550 560 LEASIEQHFW QPVYRRYKRT AF

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF94347.1. Different initiation.
PIR	A82232.
RefSeq	NP_230833.2. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KSR8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2614621.
GenomeReviews	Gene locus VC_1188 in contig AE003852_GR.
KEGG	vch:VC1188.
PATRIC	20081466. VBIVibCho83274_1136.
TIGR	VC_1188.
Phylogenomic databases
HOGENOM	HBG289821.
OMA	QDNNETL.
PhylomeDB	Q9KSR8.
ProtClustDB	PRK13529.
Family and domain databases
HAMAP	MF_01619. NAD_malic_enz. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD-bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. IPR023667. NAD_malic_enz_proteobac. [Graphical view]
Gene3D	G3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00027.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PIRSF	PIRSF000106. ME. 1 hit.
PRINTS	PR00072. MALOXRDTASE.
SMART	SM00919. Malic_M. 1 hit. [Graphical view]
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_VIBCH

Accession

Primary (citable) accession number: Q9KSR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	January 25, 2012
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program