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Protein

Succinylglutamate desuccinylase

Gene

astE

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transforms N(2)-succinylglutamate into succinate and glutamate.UniRule annotation

Catalytic activityi

N-succinyl-L-glutamate + H2O = succinate + L-glutamate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: L-arginine degradation via AST pathway

This protein is involved in step 5 of the subpathway that synthesizes L-glutamate and succinate from L-arginine.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Arginine N-succinyltransferase (VC_2617)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. N-succinylglutamate 5-semialdehyde dehydrogenase (astD)
  5. Succinylglutamate desuccinylase (astE)
This subpathway is part of the pathway L-arginine degradation via AST pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate and succinate from L-arginine, the pathway L-arginine degradation via AST pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63ZincUniRule annotation1
Metal bindingi66ZincUniRule annotation1
Metal bindingi155ZincUniRule annotation1
Active sitei219UniRule annotation1

GO - Molecular functioni

  • hydrolase activity, acting on ester bonds Source: TIGR
  • succinylglutamate desuccinylase activity Source: UniProtKB-EC
  • zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Arginine metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciVCHO:VC1242-MONOMER.
UniPathwayiUPA00185; UER00283.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinylglutamate desuccinylaseUniRule annotation (EC:3.5.1.96UniRule annotation)
Gene namesi
Name:astEUniRule annotation
Ordered Locus Names:VC_1242
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001746501 – 342Succinylglutamate desuccinylaseAdd BLAST342

Proteomic databases

PRIDEiQ9KSL4.

Interactioni

Protein-protein interaction databases

STRINGi243277.VC1242.

Structurei

Secondary structure

1342
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni10 – 14Combined sources5
Beta strandi17 – 19Combined sources3
Beta strandi24 – 27Combined sources4
Beta strandi33 – 38Combined sources6
Beta strandi41 – 45Combined sources5
Beta strandi55 – 60Combined sources6
Beta strandi62 – 65Combined sources4
Helixi68 – 81Combined sources14
Beta strandi82 – 85Combined sources4
Beta strandi89 – 94Combined sources6
Helixi98 – 102Combined sources5
Beta strandi108 – 110Combined sources3
Beta strandi117 – 119Combined sources3
Helixi125 – 140Combined sources16
Turni141 – 143Combined sources3
Helixi146 – 148Combined sources3
Beta strandi149 – 159Combined sources11
Beta strandi161 – 169Combined sources9
Helixi179 – 187Combined sources9
Beta strandi191 – 195Combined sources5
Helixi203 – 211Combined sources9
Beta strandi214 – 222Combined sources9
Turni226 – 228Combined sources3
Turni231 – 234Combined sources4
Helixi235 – 246Combined sources12
Beta strandi258 – 267Combined sources10
Beta strandi270 – 272Combined sources3
Beta strandi297 – 299Combined sources3
Beta strandi304 – 307Combined sources4
Beta strandi309 – 311Combined sources3
Beta strandi319 – 329Combined sources11
Beta strandi332 – 341Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G9DX-ray3.00A1-342[»]
ProteinModelPortaliQ9KSL4.
SMRiQ9KSL4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KSL4.

Family & Domainsi

Sequence similaritiesi

Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2988. LUCA.
KOiK05526.
OMAiFIIAHPE.

Family and domain databases

CDDicd03855. M14_ASTE. 1 hit.
HAMAPiMF_00767. Arg_catab_AstE. 1 hit.
InterProiIPR007036. Aste_AspA.
IPR016681. SuccinylGlu_desuccinylase.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF017020. AstE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KSL4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKSLFRQSF LFDSLDLDHP MVAQTVRTEQ GVTLKLHQRG VLEVIPAQTD
60 70 80 90 100
AATKNMVISC GIHGDETAPM ELLDKWIDDI VSGFQPVAER CLFIMAHPQA
110 120 130 140 150
TVRHVRFIEQ NLNRLFDDKP HTPSTELAIA DNLKVLLRQF FANTDEHSRW
160 170 180 190 200
HLDLHCAIRG SKHYSFAVSP KARHPVRSRS LMQFIEQAHI EAVMLSNAPS
210 220 230 240 250
STFSWYSAEH YAAQALTLEL GQVARLGENL LDRLLAFDLA MRDLISRHKP
260 270 280 290 300
EHLPRKSVMY RVSRTIVRLH DDFDFRFSDD VENFTAFMHG EVFGHDGDKP
310 320 330 340
LMAKNEGEAI VFPNRKVAIG QRAALMVCKV NTRYEDDQLV YD
Length:342
Mass (Da):39,093
Last modified:October 1, 2000 - v1
Checksum:iD5D646FA0F6E3796
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94401.1.
PIRiE82224.
RefSeqiNP_230887.1. NC_002505.1.
WP_000167426.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF94401; AAF94401; VC_1242.
GeneIDi2614679.
KEGGivch:VC1242.
PATRICi20081568. VBIVibCho83274_1183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94401.1.
PIRiE82224.
RefSeqiNP_230887.1. NC_002505.1.
WP_000167426.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G9DX-ray3.00A1-342[»]
ProteinModelPortaliQ9KSL4.
SMRiQ9KSL4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC1242.

Proteomic databases

PRIDEiQ9KSL4.

Protocols and materials databases

DNASUi2614679.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF94401; AAF94401; VC_1242.
GeneIDi2614679.
KEGGivch:VC1242.
PATRICi20081568. VBIVibCho83274_1183.

Phylogenomic databases

eggNOGiCOG2988. LUCA.
KOiK05526.
OMAiFIIAHPE.

Enzyme and pathway databases

UniPathwayiUPA00185; UER00283.
BioCyciVCHO:VC1242-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9KSL4.

Family and domain databases

CDDicd03855. M14_ASTE. 1 hit.
HAMAPiMF_00767. Arg_catab_AstE. 1 hit.
InterProiIPR007036. Aste_AspA.
IPR016681. SuccinylGlu_desuccinylase.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF017020. AstE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiASTE_VIBCH
AccessioniPrimary (citable) accession number: Q9KSL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.