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Protein

DNA gyrase subunit A

Gene

gyrA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.UniRule annotation

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei122O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • DNA binding Source: UniProtKB-HAMAP
  • DNA topoisomerase activity Source: TIGR
  • DNA topoisomerase type II (ATP-hydrolyzing) activity Source: TIGR

GO - Biological processi

  • DNA-dependent DNA replication Source: UniProtKB-HAMAP
  • DNA topological change Source: TIGR

Keywordsi

Molecular functionDNA-bindingUniRule annotationSAAS annotation, Isomerase, TopoisomeraseUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciVCHO:VC1258-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesi
Name:gyrAUniRule annotation
Ordered Locus Names:VC_1258Imported
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)Imported
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • chromosome Source: InterPro
  • cytoplasm Source: UniProtKB-SubCell
  • DNA topoisomerase complex (ATP-hydrolyzing) Source: TIGR

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.UniRule annotation

Protein-protein interaction databases

STRINGi243277.VC1258.

Structurei

3D structure databases

ProteinModelPortaliQ9KSJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 498TOP4cInterPro annotationAdd BLAST488

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi559 – 565GyrA-boxUniRule annotation7

Sequence similaritiesi

Belongs to the topoisomerase GyrA/ParC subunit family.UniRule annotation
Belongs to the type II topoisomerase GyrA/ParC subunit family.SAAS annotation

Phylogenomic databases

eggNOGiENOG4105C24. Bacteria.
COG0188. LUCA.
KOiK02469.
OMAiTHHWLLF.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.90.199.10. 2 hits.
HAMAPiMF_01897. GyrA. 1 hit.
InterProiView protein in InterPro
IPR005743. GyrA.
IPR006691. GyrA/parC_rep.
IPR035516. Gyrase/topoIV_suA_C.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
PfamiView protein in Pfam
PF03989. DNA_gyraseA_C. 7 hits.
PF00521. DNA_topoisoIV. 1 hit.
SMARTiView protein in SMART
SM00434. TOP4c. 1 hit.
SUPFAMiSSF101904. SSF101904. 1 hit.
SSF56719. SSF56719. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KSJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLAKEITP VNIEDELRSS YLDYAMSVIV GRALPDVRDG LKPVHRRVLF
60 70 80 90 100
AMNVLGNDWN KAYKKSARVV GDVIGKYHPH GDSAVYDTIV RMAQPFSLRY
110 120 130 140 150
MLVDGQGNFG SIDGDSAAAM RYTEVRMSKI AHELLADLDK ETVDYVPNYD
160 170 180 190 200
GTEQIPAVLP TKIPNLLING ASGIAVGMAT NIPPHNLGEV IDGCLAYIDN
210 220 230 240 250
EAITIDELMD YIPGPDFPTA ALISGRKGII DAYKTGRGKV YMRSKAEIET
260 270 280 290 300
DKNGKETIIV SEIPYQVNKA RLIEKIAELV KEKKVEGISA LRDESDKDGM
310 320 330 340 350
RIVIECKRDA VGEVVLNNLY ANTQLQTTFG INMVALDNGQ PKLFNLKEML
360 370 380 390 400
KCFVDHRREV VTRRTIFELR KARERAHILE GLALALANID EIIELIRRAA
410 420 430 440 450
TPAEAKEGLI ARGWDLGNVA AMLERAGTDA ARPEWLEPQY GIRDGKYFLT
460 470 480 490 500
EQQAQAILDL RLHKLTGLEH EKILDEYKQL LEEIAALMHI LASTERLMEV
510 520 530 540 550
IREELEAIRA GFSDARRTEI TAATHDIDME ELIAREDVVV TLSHEGYVKY
560 570 580 590 600
QLLSDYEAQR RGGKGKSATK MKEEDYIERL LVANTHDNIL LFSTRGKTYR
610 620 630 640 650
MKVYQLPLAS RTARGKPIVN LLPLEENERI TAILPVTEFS EDKFIFMATG
660 670 680 690 700
DGTVKKTSLD QFANVRANGL IALNLRDEDS LIGVDITDGE SEIMLFSKFG
710 720 730 740 750
KVVRFKESEE TAVVDENGQP VLDENGQPEI KFKGVRPMGR TAAGVRGMKL
760 770 780 790 800
ADGDQVVSLI VPKTEGDVLT VTENGYGKRT SLSEYPTKGR GTQGVVSIKV
810 820 830 840 850
SERNGSVVGA VQVAEGDEFM MITNAGTLVR TRVAEVSQVG RNTQGVTLIR
860 870 880 890
TSEGESVVGL QRIDEIEESE LPEGEEALVE NDAPVAQDDD GEQE
Length:894
Mass (Da):99,026
Last modified:October 1, 2000 - v1
Checksum:iC7EA42056A08F57F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94417.1.
PIRiE82221.
RefSeqiNP_230903.1. NC_002505.1.
WP_001281169.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF94417; AAF94417; VC_1258.
GeneIDi2614695.
KEGGivch:VC1258.
PATRICifig|243277.26.peg.1197.

Similar proteinsi

Entry informationi

Entry nameiQ9KSJ8_VIBCH
AccessioniPrimary (citable) accession number: Q9KSJ8
Entry historyiIntegrated into UniProtKB/TrEMBL: October 1, 2000
Last sequence update: October 1, 2000
Last modified: August 30, 2017
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported