Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9KRZ5 (RLMKL_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA large subunit methyltransferase K/L

Including the following 2 domains:

  1. 23S rRNA m2G2445 methyltransferase
    EC=2.1.1.173
    Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase RlmL
  2. 23S rRNA m7G2069 methyltransferase
    EC=2.1.1.264
    Alternative name(s):
    rRNA (guanine-N(7)-)-methyltransferase RlmK
Gene names
Name:rlmL
Ordered Locus Names:VC_1488
OrganismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]
Taxonomic identifier243277 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length708 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA By similarity. HAMAP-Rule MF_01858

Catalytic activity

S-adenosyl-L-methionine + guanine(2445) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(2445) in 23S rRNA. HAMAP-Rule MF_01858

S-adenosyl-L-methionine + guanine(2069) in 23S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(2069) in 23S rRNA. HAMAP-Rule MF_01858

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01858.

Sequence similarities

Belongs to the methyltransferase superfamily. RlmKL family.

Contains 1 THUMP domain.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandRNA-binding
S-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function23S rRNA (guanine(2445)-N(2))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

RNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

rRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 708708Ribosomal RNA large subunit methyltransferase K/L HAMAP-Rule MF_01858
PRO_0000366846

Regions

Domain43 – 154112THUMP

Sequences

Sequence LengthMass (Da)Tools
Q9KRZ5 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 80C9C366AA5ADB8F

FASTA70879,833
        10         20         30         40         50         60 
MNQYLAVTSN GLENLLVEEL TQLGINDAKP VQAGVKFKAT NEQIYRCCLW SRLASRFVRI 

        70         80         90        100        110        120 
VAEFKCQNDL DLYLSTTSVN WVNYFHSSKK LVVDFNGTNR EIRNSQYGAM KVKDAIVDCF 

       130        140        150        160        170        180 
TKKNLPRPSI SKDLADLHIH VRLHKENALL GIDMVGSGLH ARGYRTEAGK APLRETLAAA 

       190        200        210        220        230        240 
IILRSGWDAS KPLLDPMCGS GTLLIEAAMM AANIAPGLQR KKWGFEALED FEPELWASVK 

       250        260        270        280        290        300 
SEASVQGKRG VKKVETHFYG VDNDNRVLQT AKDNARRAGV EELISFTLGD AAKVKRPENF 

       310        320        330        340        350        360 
AEGIVICNPP YGERLGTHPG LIALYTAFGA QLKAEFGGCH ASIFSSSDEL LSCLRMRADK 

       370        380        390        400        410        420 
QFKLNNGALP CHQKNYTIAM REQNSVSNEG TQEILIAPDF ANRLKKNFNK IGKWAKREGL 

       430        440        450        460        470        480 
DCFRLYDADL PEYNVAIDVY QDHLMIQEYA APKDIPEEKA KRRLTDIIRA AIQVLDVDAN 

       490        500        510        520        530        540 
NVVLKVRERQ KGTSQYEKLG QQAQTMQITE YGVKLIVNLY DYLDTGLFLD HKITRRRLGQ 

       550        560        570        580        590        600 
MAQGKDFLNL FAYTGSATVH AACGGAKSTT TVDMSKTYLE WAKENMQLNG QVGRQHQYVQ 

       610        620        630        640        650        660 
ADCLQWLANA QSQYDLIFID PPTFSNSKRM EQTFDVQRDH VTLMTNLKRL LRPEGTIVFS 

       670        680        690        700 
NNKRHFKMDM EALHALGLNA QNISHQTLPL DFERNKQIHN CWLITHQS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE003852 Genomic DNA. Translation: AAF94643.1.
PIRA82195.
RefSeqNP_231129.1. NC_002505.1.

3D structure databases

ProteinModelPortalQ9KRZ5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243277.VC1488.

Protocols and materials databases

DNASU2613994.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF94643; AAF94643; VC_1488.
GeneID2613994.
KEGGvch:VC1488.
PATRIC20082034. VBIVibCho83274_1416.

Phylogenomic databases

eggNOGCOG0116.
KOK12297.
OMANKRGFKM.
OrthoDBEOG6HJ238.

Enzyme and pathway databases

BioCycVCHO:VC1488-MONOMER.

Family and domain databases

Gene3D3.40.50.150. 3 hits.
HAMAPMF_01858. 23SrRNA_methyltr_KL.
InterProIPR017244. 23SrRNA_methyltr_KL.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR000241. RNA_methylase_dom.
IPR019614. SAM-dep_methyl-trfase.
IPR029063. SAM-dependent_MTases-like.
IPR004114. THUMP.
[Graphical view]
PfamPF10672. Methyltrans_SAM. 1 hit.
PF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
PIRSFPIRSF037618. RNA_Mtase_bacteria_prd. 1 hit.
SMARTSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 3 hits.
PROSITEPS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRLMKL_VIBCH
AccessionPrimary (citable) accession number: Q9KRZ5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families