Q9KRS6 (KATG_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	VC_1560

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Catalase-peroxidase HAMAP MF_01961		PRO_0000354947
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	267	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 226		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252) By similarity
	Cross-link	226 ↔ 252		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KRS6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C53E123D12F6DB51	FASTA	724	80,651
	10 20 30 40 50 60 MEHNKAGSSG QCPVMHGGLT SASMSNMDWW PKALNLDILH QHDSKTNPLG ADFNYREELK 70 80 90 100 110 120 KLDVEALKRD LKALMTNSQE WWPADWGHYG GLMIRMAWHS AGTYRIADGR GGGGTGNQRF 130 140 150 160 170 180 APLNSWPDNA NLDKARRLLW PIKQKYGNKI SWADLMILAG NMAYESMGLK TFGFAFGRED 190 200 210 220 230 240 IWHPEKDIYW GSEKEWLAKS GGENSRYSGQ RDLENPLAAV MMGLIYVNPE GVDGNPDPLK 250 260 270 280 290 300 TAQDMRVTFA RMAMNDEETV ALTAGGHTVG KAHGNGKASN LGPDPEGAEL HEQGLGWNNH 310 320 330 340 350 360 TSRGIGRNTV TSGIEGAWTT HPTRWDNEYF YLLLSYEWQL TKSPAGAWQW EPVNIKEEDK 370 380 390 400 410 420 PVDVEDPSIR YNPMMTDADM ALKIDPEYRK ISERFYKDPA YFSEVFARAW FKLTHRDMGP 430 440 450 460 470 480 KARYFGPDVP AEDLIWQDPV PAGRKDYDVN AVKAKIAASG LSISEMVSTA WDSARTFRGS 490 500 510 520 530 540 DKRGGANGAR IRLAPQKDWE GNEPARLGKV LAVLEKIAAE SGISIADTIV LAGNVGIEQA 550 560 570 580 590 600 AKAAGVNVTV PFAPGRGDAT IEQTDVESFE VLEPLADGFR NWQKKHYVVT PEEMLLDKAQ 610 620 630 640 650 660 LLRLTAPEMT VLIGGMRVLG TNYGGSQHGV FTDRVGALTN DFFVNLTDMS YTWKPTGRNS 670 680 690 700 710 720 YEIVERKSGK VKWTATRVDL VFGSNSILRA YAEVYAQDDN KEKFVKDFVA AWTKVMNADR FDLV

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF94714.1.
PIR	B82186.
RefSeq	NP_231200.1. NC_002505.1.
3D structure databases
HSSP	HSSP built from PDB template 1ITK based on UniProtKB O59651.
ProteinModelPortal	Q9KRS6.
SMR	Q9KRS6. Positions 25-723.
ModBase	Search...
Protein family/group databases
PeroxiBase	2445. VchCP01_N16961.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2613939.
GenomeReviews	Gene locus VC_1560 in contig AE003852_GR.
KEGG	vch:VC1560.
PATRIC	20082179. VBIVibCho83274_1488.
TIGR	VC_1560.
Phylogenomic databases
HOGENOM	HBG285610.
OMA	WPNALNL.
PhylomeDB	Q9KRS6.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_VIBCH

Accession

Primary (citable) accession number: Q9KRS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program