Q9KRK5 (RSUA_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal small subunit pseudouridine synthase A
EC=5.4.99.19

Alternative name(s):
16S pseudouridylate 516 synthase
16S rRNA pseudouridine(516) synthase
rRNA pseudouridylate synthase A
rRNA-uridine isomerase A

Gene names

Name:	rsuA
Ordered Locus Names:	VC_1635

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1 › Vibrio cholerae O1 biovar El Tor

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil-516 in 16S ribosomal RNA By similarity.
Catalytic activity	16S rRNA uridine(516) = 16S rRNA pseudouridine(516).
Sequence similarities	Belongs to the pseudouridine synthase RsuA family. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	rRNA processing
Ligand	RNA-binding
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro rRNA modification Inferred from sequence or structural similarity Ref.1. Source: TIGR
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW pseudouridine synthase activity Inferred from sequence or structural similarity Ref.1. Source: TIGR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Ribosomal small subunit pseudouridine synthase A		PRO_0000099975
Regions
	Domain	1 – 67	67	S4 RNA-binding
Sites
	Active site	101	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KRK5 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 0B46DCE8E3AB497C	FASTA	231	26,362
	10 20 30 40 50 60 MRLDKFLCDA LGTTRKEATQ LLKSGEVTVD DVVQKSGAFK LKETSCVEWQ GREITLHGPR 70 80 90 100 110 120 YIMMHKPDGV VCSHEDGFNQ TALTLLDEVN IQDLHFAGRL DVDTTGLLLI TDDGQWSHRV 130 140 150 160 170 180 TSPKHKCEKT YRVWLADPIQ ADYAEQFNRG IELRGERELT LPAQLEVISE TEVLLTIHEG 190 200 210 220 230 KYHQVKRMFA ALGNKVIGLH RERIGQITLD ETLEPGEYRY LTEEEVASIW K

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF94786.1.
PIR	A82176.
RefSeq	NP_231272.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KRK5.
SMR	Q9KRK5. Positions 1-229.
ModBase	Search...
Protein-protein interaction databases
STRING	243277.VC1635.
Protocols and materials databases
DNASU	2613890.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF94786; AAF94786; VC_1635.
GeneID	2613890.
KEGG	vch:VC1635.
PATRIC	20082334. VBIVibCho83274_1563.
Phylogenomic databases
eggNOG	COG1187.
KO	K06183.
OMA	YTHRVIS.
ProtClustDB	CLSK874466.
Family and domain databases
Gene3D	3.10.290.10. 1 hit.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR000748. PsdUridine_synth_RsuA/RluB/E/F. IPR018496. PsdUridine_synth_RsuA/RluB_CS. IPR006145. PsdUridine_synth_RsuA/RluD. IPR002942. S4_RNA-bd. [Graphical view]
Pfam	PF00849. PseudoU_synth_2. 1 hit. PF01479. S4. 1 hit. [Graphical view]
SMART	SM00363. S4. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00093. TIGR00093. 1 hit.
PROSITE	PS01149. PSI_RSU. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSUA_VIBCH

Accession

Primary (citable) accession number: Q9KRK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program