Reviewed, UniProtKB/Swiss-Prot Q9KRF0 (TORA_VIBCH)

Names and origin

Protein names

Recommended name:
    Trimethylamine-N-oxide reductase
      Short name=TMAO reductase
      Short name=Trimethylamine oxidase
    EC=1.7.2.3

Gene names

Name:	torA
Ordered Locus Names:	VC_1692

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	820 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions By similarity.
Catalytic activity	Trimethylamine + 2 (ferricytochrome c)-subunit + H2O = trimethylamine N-oxide + 2 (ferrocytochrome c)-subunit + 2 H+.
Cofactor	Molybdenum (molybdopterin) By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW trimethylamine-N-oxide reductase (cytochrome c) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Tat-type signal Potential
	Chain	34 – 820	787	Trimethylamine-N-oxide reductase		PRO_0000019159

Sequences

	Sequence		Length	Mass (Da)
	Q9KRF0-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 6C1BF9FE639DBD82	FASTA	820	92,305
	10 20 30 40 50 60 MAITRRSFLK GVATTSAASI IGPSLLTSVS AQAAETTGTW KVSGSHWGAF RAHIYGGKVQ 70 80 90 100 110 120 ELKALELDTH PTEMLNGIQG ILYSPSRVRY PMVRLDWLKK HKYSAETRGN NRFIRVTWDE 130 140 150 160 170 180 AIDLFYRELE RVQKQYGPWA LHAGQTGWNQ TGAFHNCTAM MQRAVGMHGN YITKVGDYST 190 200 210 220 230 240 GAGQTIMPYV LGSTEVYAQG TSWSEILDNS DNIILWANDP VKNLQVGWNC ETHQSFGYLD 250 260 270 280 290 300 QLKEKVAKGE INVVSVDPVK NKTQRFLQND HLYINPQTDV AFMLALAHVL YTENLYDKKF 310 320 330 340 350 360 IETYCLGFEE FIPYVLGKSK DKVEKTPEWA ATICGVKPDA IRDFARMLVN GRTQLLFGWC 370 380 390 400 410 420 IQRQEHGEQP YWMGAVLAAM IGQIGLPGGG ISYGHHYSGI GVPSTGFAGP GGFPRNLDQG 430 440 450 460 470 480 AKPKWDNNDF NGYSRTIPVA RWIDAILEPG KKINHNGNTV TLPGFKMMVI SGCNPWHHHQ 490 500 510 520 530 540 DRNKMKRAFQ KLETVVTIDF SWTATCRFSD IVLPACTQWE RNDIDSYGSY SGKGLIAMHR 550 560 570 580 590 600 LVDPLFQSRT DFEIMTELTR RFGREKEYTR GMDEMEWVRS LYDECKKANE GKFAMPEFEE 610 620 630 640 650 660 FWEKGFLDFG TGTPWVRHAD FRKDPEINAL GTPSGFIEIT SRKIGRYGYE HCQEHPMWFE 670 680 690 700 710 720 KTERSHGGPG SDKHPFWLQS CHPDKRLHSQ MCEAEAFRAT YAVQGREPVY INPLDAKAKG 730 740 750 760 770 780 IKDGDLVRVF NDRGQLLAGA VLSDSYPRGV IRIEEGAWYG PLTEKVGAIC TYGDPNTLTL 790 800 810 820 DLGTSELAQA TSANTCIVDF EKFRGEVPPV TSFGGPIEVI

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE003852 Genomic DNA. Translation: AAF94842.1.
PIR	G82168.
RefSeq	NP_231328.1.
3D structure databases
HSSP	HSSP built from PDB template 1TMO based on UniProtKB O87948.
ModBase	Search...
Genome annotation databases
GeneID	2613823.
GenomeReviews	Gene locus VC_1692 in contig AE003852_GR.
KEGG	vch:VC1692.
TIGR	VC_1692.
Phylogenomic databases
HOGENOM	Q9KRF0.
OMA	Q9KRF0. RDFARML.
Enzyme and pathway databases
BRENDA	1.7.2.3. 19019.
Family and domain databases
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR006311. Tat. IPR011887. TorA. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit. TIGR02164. torA. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. False negative. PS00490. MOLYBDOPTERIN_PROK_2. 1 hit. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TORA_VIBCH

Accession

Primary (citable) accession number: Q9KRF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)