Reviewed, UniProtKB/Swiss-Prot Q9KRB2 (TOP1_VIBCH)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	VC_1730

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	876 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 876	876	DNA topoisomerase 1		PRO_0000145171
Regions
	Zinc finger	668 – 695	28	C4-type 1
	Zinc finger	717 – 742	26	C4-type 2
Sites
	Active site	327	1	For DNA cleavage activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KRB2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8A5BC9148CA1B753	FASTA	876	97,910
	10 20 30 40 50 60 MGKSLVIVES PAKAKTINKY LGKDFIVKSS VGHVRDLPTA GQTATPTGKA AAASTKKAST 70 80 90 100 110 120 TDKEQQKREK ERAALIKKMG VDPYHGWQAN YQILPGKEKV VAELQKLAKD ADSVYLATDL 130 140 150 160 170 180 DREGEAIAWH LREIIGGDEK RYKRVVFNEI TKNAIQQAFK QPGELNMDGV NAQQARRFMD 190 200 210 220 230 240 RVVGFMVSPL LWKKVARGLS AGRVQSVAVK LLVEREREIK AFVPEEFWDI NADTLTADKQ 250 260 270 280 290 300 DFRLLVAQKD GLAFKPVNET EALAAVAALN KAQYEVCKRE DKPTTSKPSA PFITSTLQQA 310 320 330 340 350 360 ASTRLGYGVK KTMMLAQRLY EAGYITYMRT DSTNLSAEAV ENLRGYITKH YGEAYLPSQP 370 380 390 400 410 420 NVYGSKQNAQ EAHEAIRPSD VTVTADDLEG MEADAHKLYA LIWNQFVACQ MTPAQYDSTT 430 440 450 460 470 480 VSVKAAEYTL KAKGRILKFD GWTRVQRPLG KNEDQILPPV KVGDSLKLVS LDPKQHFTKP 490 500 510 520 530 540 PARYTEAALV KELEKRGIGR PSTYASIIST IQDRGYVKVD QRRFFAEKMG EIVTDRLDEN 550 560 570 580 590 600 FDDLMNYDFT ARMEEKLDQI AEGEVNWTAV LDEFFADFSR DLETAEQDES LGGMKPNHIV 610 620 630 640 650 660 MTNILCPTCS RPMGIRTAST GVFLGCSGYG LPPKERCKTT INLGDEEGVI NVLEEDVETA 670 680 690 700 710 720 ALRAKKRCPI CETAMDAYLI DDKRKLHVCG NNPNCEGFIV EEGEFKVKGY EGPTVECDKC 730 740 750 760 770 780 GSDMVLKNGR FGKYMGCTND ACKNTRKILK NGEVAPPKEE PVHFPELPCE NSDAYFVLRD 790 800 810 820 830 840 GASGLFFAAS NFPKSRETRA PLVEELKRFA ERLPEKYQYL TSAPAHDPDG LPAVVRFSRK 850 860 870 EKEHYVRTET EGKPSGWMAV YQEGKWLVTD KRKNAK

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE003852 Genomic DNA. Translation: AAF94880.1.
PIR	B82163.
RefSeq	NP_231366.1.
3D structure databases
HSSP	HSSP built from PDB template 1CY7 based on UniProtKB P06612.
SMR	Q9KRB2. Positions 2-599.
ModBase	Search...
Genome annotation databases
GeneID	2613735.
GenomeReviews	Gene locus VC_1730 in contig AE003852_GR.
KEGG	vch:VC1730.
TIGR	VC_1730.
Phylogenomic databases
HOGENOM	Q9KRB2.
OMA	RKFYGCS.
Enzyme and pathway databases
BRENDA	5.99.1.2. 19019.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac. IPR013263. TopoI_Znr_bac. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF08272. Topo_Zn_Ribbon. 2 hits. PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01051. topA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_VIBCH

Accession

Primary (citable) accession number: Q9KRB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)