Q9KRA7 (LFTR_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucyl/phenylalanyl-tRNA--protein transferase
EC=2.3.2.6

Alternative name(s):
L/F-transferase
Leucyltransferase
Phenyalanyltransferase

Gene names

Name:	aat
Ordered Locus Names:	VC_1735

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1 › Vibrio cholerae O1 biovar El Tor

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine By similarity. HAMAP-Rule MF_00688
Catalytic activity	L-leucyl-tRNA + protein = tRNA + L-leucyl-protein. HAMAP-Rule MF_00688 L-phenylalanyl-tRNA + protein = tRNA + L-phenylalanyl-protein. HAMAP-Rule MF_00688
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the L/F-transferase family.
Sequence caution	The sequence AAB87633.1 differs from that shown. Reason: Frameshift at position 206.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein catabolic process Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from sequence or structural similarity Ref.1. Source: TIGR
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	leucyltransferase activity Inferred from electronic annotation. Source: HAMAP peptidase activity Inferred from sequence or structural similarity Ref.1. Source: TIGR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Leucyl/phenylalanyl-tRNA--protein transferase HAMAP-Rule MF_00688		PRO_0000207247

Sequences

	Sequence		Length	Mass (Da)
	Q9KRA7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 24AF885AE888D515	FASTA	243	27,569
	10 20 30 40 50 60 MAIYLTELSP ETLTFPSPFT ALDDPNGLLA FGGDLRLERI WAAYQQGIFP WYGPEDPILW 70 80 90 100 110 120 WSPSPRAVFD PTRFQPAKSV KKFQRKHQYR VSVNHATSQV IEQCALTRPA DQRWLNDSMR 130 140 150 160 170 180 HAYGELAKQG RCHSVEVWQG EQLVGGLYGI SVGQLFCGES MFSLATNASK IALWYFCDHF 190 200 210 220 230 240 SRHQGQLIDC QVMNPHLQSL GATTLSREQF IQSLLSFKEK QVLSGCFETQ WLATPTSPCA FED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.
[2]	"Substrate recognition by the leucyl/phenylalanyl-tRNA-protein transferase. Conservation within the enzyme family and localization to the trypsin-resistant domain." Ichetovkin I.E., Abramochkin G., Shrader T.E. J. Biol. Chem. 272:33009-33014(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-208.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF94885.1. AF035002 Genomic DNA. Translation: AAB87633.1. Frameshift.
PIR	G82163.
RefSeq	NP_231371.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KRA7.
ModBase	Search...
Protein-protein interaction databases
STRING	243277.VC1735.
Protocols and materials databases
DNASU	2613740.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF94885; AAF94885; VC_1735.
GeneID	2613740.
KEGG	vch:VC1735.
PATRIC	20082524. VBIVibCho83274_1658.
Phylogenomic databases
eggNOG	COG2360.
KO	K00684.
OMA	MFYGESM.
ProtClustDB	PRK00301.
Family and domain databases
HAMAP	MF_00688. Leu_Phe_trans.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR004616. Leu/Phe-tRNA_Trfase. [Graphical view]
Pfam	PF03588. Leu_Phe_trans. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
TIGRFAMs	TIGR00667. aat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LFTR_VIBCH

Accession

Primary (citable) accession number: Q9KRA7
Secondary accession number(s): O50259

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program