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Protein

3-oxoacyl-[acyl-carrier-protein] synthase 2

Gene

fabF

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Has a preference for short chain acid substrates and may function to supply the octanoic substrates for lipoic acid biosynthesis (By similarity).By similarity

Catalytic activityi

(Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641PROSITE-ProRule annotation

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: TIGR
  2. beta-ketoacyl-acyl-carrier-protein synthase II activity Source: UniProtKB-EC

GO - Biological processi

  1. fatty acid biosynthetic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciVCHO:VC2019-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 2 (EC:2.3.1.179)
Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase II
Beta-ketoacyl-ACP synthase II
Short name:
KAS II
Gene namesi
Name:fabF
Ordered Locus Names:VC_2019
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 4144133-oxoacyl-[acyl-carrier-protein] synthase 2PRO_0000180327Add
BLAST

Proteomic databases

PRIDEiQ9KQH9.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi243277.VC2019.

Structurei

Secondary structure

1
414
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 1510Combined sources
Beta strandi18 – 203Combined sources
Helixi21 – 299Combined sources
Beta strandi35 – 373Combined sources
Beta strandi50 – 523Combined sources
Helixi59 – 613Combined sources
Helixi65 – 684Combined sources
Helixi73 – 8917Combined sources
Turni95 – 973Combined sources
Helixi98 – 1003Combined sources
Beta strandi101 – 1066Combined sources
Helixi112 – 12514Combined sources
Helixi127 – 1293Combined sources
Helixi134 – 1385Combined sources
Helixi142 – 15110Combined sources
Helixi163 – 1653Combined sources
Helixi166 – 18015Combined sources
Beta strandi184 – 1929Combined sources
Helixi197 – 2059Combined sources
Helixi216 – 2194Combined sources
Beta strandi235 – 2439Combined sources
Helixi244 – 2496Combined sources
Beta strandi256 – 26510Combined sources
Beta strandi270 – 2723Combined sources
Helixi278 – 29114Combined sources
Helixi295 – 2973Combined sources
Beta strandi298 – 3025Combined sources
Helixi309 – 33022Combined sources
Beta strandi332 – 3343Combined sources
Helixi337 – 3404Combined sources
Helixi344 – 3463Combined sources
Helixi347 – 36115Combined sources
Beta strandi371 – 3733Combined sources
Beta strandi383 – 3853Combined sources
Beta strandi394 – 4018Combined sources
Turni402 – 4043Combined sources
Beta strandi405 – 4139Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4JRHX-ray2.20A/B1-414[»]
4JRMX-ray1.75A/B/C/D1-414[»]
ProteinModelPortaliQ9KQH9.
SMRiQ9KQH9. Positions 3-414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-ketoacyl-ACP synthases family.Curated

Phylogenomic databases

eggNOGiCOG0304.
KOiK09458.
OMAiENPDPDC.
OrthoDBiEOG6DG2SR.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000447. KAS_II. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR03150. fabF. 1 hit.
PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9KQH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRRVVVTG MGMLSPVGNT VESSWKALLA GQSGIVNIEH FDTTNFSTRF
60 70 80 90 100
AGLVKGFDCE QYMSKKDARK MDLFIQYGIA AGIQALEDSG LEVNEENAAR
110 120 130 140 150
IGVAIGSGIG GLELIETGHQ ALIEKGPRKV SPFFVPSTIV NMIAGNLSIM
160 170 180 190 200
RGLRGPNIAI STACTTGLHN IGHAARMIAY GDADAMVAGG AEKASTPLGM
210 220 230 240 250
AGFGAAKALS TRNDEPQKAS RPWDKDRDGF VLGDGAGIMV LEEYEHAKAR
260 270 280 290 300
GAKIYAEVVG FGMSGDAYHM TSPSEDGSGG ALAMEAAMRD AGVTGEQIGY
310 320 330 340 350
VNAHGTSTPA GDVAEVKGIK RALGEAGTKQ VLVSSTKSMT GHLLGAAGSV
360 370 380 390 400
EAIITVMSLV DQMVPPTINL DNPEEGLGVD LVPHVARKVE SMEYAMCNSF
410
GFGGTNGSLI FKRM
Length:414
Mass (Da):43,208
Last modified:January 23, 2007 - v3
Checksum:iEB5942D01274ED1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95167.1.
PIRiD82128.
RefSeqiNP_231653.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95167; AAF95167; VC_2019.
GeneIDi2613398.
KEGGivch:VC2019.
PATRICi20083070. VBIVibCho83274_1929.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95167.1.
PIRiD82128.
RefSeqiNP_231653.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4JRHX-ray2.20A/B1-414[»]
4JRMX-ray1.75A/B/C/D1-414[»]
ProteinModelPortaliQ9KQH9.
SMRiQ9KQH9. Positions 3-414.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC2019.

Proteomic databases

PRIDEiQ9KQH9.

Protocols and materials databases

DNASUi2613398.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF95167; AAF95167; VC_2019.
GeneIDi2613398.
KEGGivch:VC2019.
PATRICi20083070. VBIVibCho83274_1929.

Phylogenomic databases

eggNOGiCOG0304.
KOiK09458.
OMAiENPDPDC.
OrthoDBiEOG6DG2SR.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciVCHO:VC2019-MONOMER.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000447. KAS_II. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR03150. fabF. 1 hit.
PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiFABF_VIBCH
AccessioniPrimary (citable) accession number: Q9KQH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.