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Q9KQH7

- FABG_VIBCH

UniProt

Q9KQH7 - FABG_VIBCH

Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (20 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

    Catalytic activityi

    (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei37 – 371NADPBy similarity
    Binding sitei86 – 861NADP; via carbonyl oxygenBy similarity
    Binding sitei138 – 1381SubstrateBy similarity
    Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation
    Binding sitei184 – 1841NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 154NADPBy similarity
    Nucleotide bindingi59 – 602NADPBy similarity
    Nucleotide bindingi151 – 1555NADPBy similarity

    GO - Molecular functioni

    1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB
    2. NAD binding Source: InterPro
    3. NADP binding Source: UniProtKB

    GO - Biological processi

    1. fatty acid biosynthetic process Source: TIGR
    2. fatty acid elongation Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
    Alternative name(s):
    3-ketoacyl-acyl carrier protein reductase
    Beta-Ketoacyl-acyl carrier protein reductase
    Beta-ketoacyl-ACP reductase
    Gene namesi
    Name:fabG
    Ordered Locus Names:VC_2021
    OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
    Taxonomic identifieri243277 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000000584: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2442443-oxoacyl-[acyl-carrier-protein] reductase FabGPRO_0000054694Add
    BLAST

    Proteomic databases

    PRIDEiQ9KQH7.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi243277.VC2021.

    Structurei

    Secondary structure

    1
    244
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 126
    Helixi16 – 2712
    Beta strandi31 – 388
    Helixi39 – 4911
    Helixi50 – 523
    Beta strandi53 – 575
    Helixi63 – 7715
    Beta strandi81 – 855
    Helixi95 – 973
    Helixi100 – 11011
    Helixi112 – 12817
    Beta strandi131 – 1366
    Helixi140 – 1434
    Helixi149 – 16921
    Helixi170 – 1723
    Beta strandi174 – 1818
    Beta strandi183 – 1864
    Helixi187 – 1904
    Helixi194 – 2018
    Helixi212 – 22312
    Helixi225 – 2273
    Beta strandi234 – 2385

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3RROX-ray2.00A/B1-244[»]
    3RSHX-ray1.95A/B1-244[»]
    3U09X-ray1.75A/B1-244[»]
    4I08X-ray2.06A/B1-244[»]
    ProteinModelPortaliQ9KQH7.
    SMRiQ9KQH7. Positions 2-244.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    KOiK00059.
    OMAiDEFGAID.
    OrthoDBiEOG6N3CR8.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR011284. 3oxo_ACP_reduc.
    IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000126. 11-beta-HSD1. 1 hit.
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9KQH7-1 [UniParc]FASTAAdd to Basket

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    MNLEGKVALV TGASRGIGKA IAELLAERGA KVIGTATSES GAQAISDYLG    50
    DNGKGMALNV TNPESIEAVL KAITDEFGGV DILVNNAGIT RDNLLMRMKE 100
    EEWSDIMETN LTSIFRLSKA VLRGMMKKRQ GRIINVGSVV GTMGNAGQAN 150
    YAAAKAGVIG FTKSMAREVA SRGVTVNTVA PGFIETDMTK ALNDEQRTAT 200
    LAQVPAGRLG DPREIASAVA FLASPEAAYI TGETLHVNGG MYMI 244
    Length:244
    Mass (Da):25,566
    Last modified:June 20, 2001 - v2
    Checksum:i9FB2E8278D7CC3CE
    GO

    Sequence cautioni

    The sequence AAF95169.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE003852 Genomic DNA. Translation: AAF95169.1. Different initiation.
    PIRiF82128.
    RefSeqiNP_231655.1. NC_002505.1.

    Genome annotation databases

    EnsemblBacteriaiAAF95169; AAF95169; VC_2021.
    GeneIDi2613400.
    KEGGivch:VC2021.
    PATRICi20083074. VBIVibCho83274_1931.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE003852 Genomic DNA. Translation: AAF95169.1 . Different initiation.
    PIRi F82128.
    RefSeqi NP_231655.1. NC_002505.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3RRO X-ray 2.00 A/B 1-244 [» ]
    3RSH X-ray 1.95 A/B 1-244 [» ]
    3U09 X-ray 1.75 A/B 1-244 [» ]
    4I08 X-ray 2.06 A/B 1-244 [» ]
    ProteinModelPortali Q9KQH7.
    SMRi Q9KQH7. Positions 2-244.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243277.VC2021.

    Proteomic databases

    PRIDEi Q9KQH7.

    Protocols and materials databases

    DNASUi 2613400.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF95169 ; AAF95169 ; VC_2021 .
    GeneIDi 2613400.
    KEGGi vch:VC2021.
    PATRICi 20083074. VBIVibCho83274_1931.

    Phylogenomic databases

    eggNOGi COG1028.
    KOi K00059.
    OMAi DEFGAID.
    OrthoDBi EOG6N3CR8.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR011284. 3oxo_ACP_reduc.
    IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000126. 11-beta-HSD1. 1 hit.
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    TIGRFAMsi TIGR01830. 3oxo_ACP_reduc. 1 hit.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39315 / El Tor Inaba N16961.

    Entry informationi

    Entry nameiFABG_VIBCH
    AccessioniPrimary (citable) accession number: Q9KQH7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: June 20, 2001
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3