Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37NADPBy similarity1
Binding sitei86NADP; via carbonyl oxygenBy similarity1
Binding sitei138SubstrateBy similarity1
Active sitei151Proton acceptorPROSITE-ProRule annotation1
Binding sitei184NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 15NADPBy similarity4
Nucleotide bindingi59 – 60NADPBy similarity2
Nucleotide bindingi151 – 155NADPBy similarity5

GO - Molecular functioni

GO - Biological processi

  • fatty acid biosynthetic process Source: TIGR
  • fatty acid elongation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase
Gene namesi
Name:fabG
Ordered Locus Names:VC_2021
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000546941 – 2443-oxoacyl-[acyl-carrier-protein] reductase FabGAdd BLAST244

Proteomic databases

PRIDEiQ9KQH7.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi243277.VC2021.

Structurei

Secondary structure

1244
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 12Combined sources6
Helixi16 – 27Combined sources12
Beta strandi31 – 38Combined sources8
Helixi39 – 49Combined sources11
Helixi50 – 52Combined sources3
Beta strandi53 – 57Combined sources5
Helixi63 – 77Combined sources15
Beta strandi81 – 85Combined sources5
Helixi95 – 97Combined sources3
Helixi100 – 110Combined sources11
Helixi112 – 128Combined sources17
Beta strandi131 – 136Combined sources6
Helixi140 – 143Combined sources4
Helixi149 – 169Combined sources21
Helixi170 – 172Combined sources3
Beta strandi174 – 181Combined sources8
Beta strandi183 – 186Combined sources4
Helixi187 – 190Combined sources4
Helixi194 – 201Combined sources8
Helixi212 – 223Combined sources12
Helixi225 – 227Combined sources3
Beta strandi234 – 238Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RROX-ray2.00A/B1-244[»]
3RSHX-ray1.95A/B1-244[»]
3U09X-ray1.75A/B1-244[»]
4I08X-ray2.06A/B1-244[»]
4WJZX-ray2.40A/B/C/D1-244[»]
5ENDX-ray2.55A/B1-244[»]
ProteinModelPortaliQ9KQH7.
SMRiQ9KQH7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CHR. Bacteria.
ENOG410XNW1. LUCA.
KOiK00059.
OMAiMKDEDWN.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KQH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLEGKVALV TGASRGIGKA IAELLAERGA KVIGTATSES GAQAISDYLG
60 70 80 90 100
DNGKGMALNV TNPESIEAVL KAITDEFGGV DILVNNAGIT RDNLLMRMKE
110 120 130 140 150
EEWSDIMETN LTSIFRLSKA VLRGMMKKRQ GRIINVGSVV GTMGNAGQAN
160 170 180 190 200
YAAAKAGVIG FTKSMAREVA SRGVTVNTVA PGFIETDMTK ALNDEQRTAT
210 220 230 240
LAQVPAGRLG DPREIASAVA FLASPEAAYI TGETLHVNGG MYMI
Length:244
Mass (Da):25,566
Last modified:June 20, 2001 - v2
Checksum:i9FB2E8278D7CC3CE
GO

Sequence cautioni

The sequence AAF95169 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95169.1. Different initiation.
PIRiF82128.
RefSeqiNP_231655.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95169; AAF95169; VC_2021.
GeneIDi2613400.
KEGGivch:VC2021.
PATRICi20083074. VBIVibCho83274_1931.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95169.1. Different initiation.
PIRiF82128.
RefSeqiNP_231655.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RROX-ray2.00A/B1-244[»]
3RSHX-ray1.95A/B1-244[»]
3U09X-ray1.75A/B1-244[»]
4I08X-ray2.06A/B1-244[»]
4WJZX-ray2.40A/B/C/D1-244[»]
5ENDX-ray2.55A/B1-244[»]
ProteinModelPortaliQ9KQH7.
SMRiQ9KQH7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC2021.

Proteomic databases

PRIDEiQ9KQH7.

Protocols and materials databases

DNASUi2613400.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF95169; AAF95169; VC_2021.
GeneIDi2613400.
KEGGivch:VC2021.
PATRICi20083074. VBIVibCho83274_1931.

Phylogenomic databases

eggNOGiENOG4105CHR. Bacteria.
ENOG410XNW1. LUCA.
KOiK00059.
OMAiMKDEDWN.

Enzyme and pathway databases

UniPathwayiUPA00094.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFABG_VIBCH
AccessioniPrimary (citable) accession number: Q9KQH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: November 2, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.