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Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371NADPBy similarity
Binding sitei86 – 861NADP; via carbonyl oxygenBy similarity
Binding sitei138 – 1381SubstrateBy similarity
Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation
Binding sitei184 – 1841NADP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 154NADPBy similarity
Nucleotide bindingi59 – 602NADPBy similarity
Nucleotide bindingi151 – 1555NADPBy similarity

GO - Molecular functioni

GO - Biological processi

  • fatty acid biosynthetic process Source: TIGR
  • fatty acid elongation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase
Gene namesi
Name:fabG
Ordered Locus Names:VC_2021
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2442443-oxoacyl-[acyl-carrier-protein] reductase FabGPRO_0000054694Add
BLAST

Proteomic databases

PRIDEiQ9KQH7.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi243277.VC2021.

Structurei

Secondary structure

1
244
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 126Combined sources
Helixi16 – 2712Combined sources
Beta strandi31 – 388Combined sources
Helixi39 – 4911Combined sources
Helixi50 – 523Combined sources
Beta strandi53 – 575Combined sources
Helixi63 – 7715Combined sources
Beta strandi81 – 855Combined sources
Helixi95 – 973Combined sources
Helixi100 – 11011Combined sources
Helixi112 – 12817Combined sources
Beta strandi131 – 1366Combined sources
Helixi140 – 1434Combined sources
Helixi149 – 16921Combined sources
Helixi170 – 1723Combined sources
Beta strandi174 – 1818Combined sources
Beta strandi183 – 1864Combined sources
Helixi187 – 1904Combined sources
Helixi194 – 2018Combined sources
Helixi212 – 22312Combined sources
Helixi225 – 2273Combined sources
Beta strandi234 – 2385Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RROX-ray2.00A/B1-244[»]
3RSHX-ray1.95A/B1-244[»]
3U09X-ray1.75A/B1-244[»]
4I08X-ray2.06A/B1-244[»]
4WJZX-ray2.40A/B/C/D1-244[»]
ProteinModelPortaliQ9KQH7.
SMRiQ9KQH7. Positions 2-244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
KOiK00059.
OMAiTGQELHV.
OrthoDBiEOG6N3CR8.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KQH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLEGKVALV TGASRGIGKA IAELLAERGA KVIGTATSES GAQAISDYLG
60 70 80 90 100
DNGKGMALNV TNPESIEAVL KAITDEFGGV DILVNNAGIT RDNLLMRMKE
110 120 130 140 150
EEWSDIMETN LTSIFRLSKA VLRGMMKKRQ GRIINVGSVV GTMGNAGQAN
160 170 180 190 200
YAAAKAGVIG FTKSMAREVA SRGVTVNTVA PGFIETDMTK ALNDEQRTAT
210 220 230 240
LAQVPAGRLG DPREIASAVA FLASPEAAYI TGETLHVNGG MYMI
Length:244
Mass (Da):25,566
Last modified:June 20, 2001 - v2
Checksum:i9FB2E8278D7CC3CE
GO

Sequence cautioni

The sequence AAF95169.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95169.1. Different initiation.
PIRiF82128.
RefSeqiNP_231655.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95169; AAF95169; VC_2021.
GeneIDi2613400.
KEGGivch:VC2021.
PATRICi20083074. VBIVibCho83274_1931.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95169.1. Different initiation.
PIRiF82128.
RefSeqiNP_231655.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RROX-ray2.00A/B1-244[»]
3RSHX-ray1.95A/B1-244[»]
3U09X-ray1.75A/B1-244[»]
4I08X-ray2.06A/B1-244[»]
4WJZX-ray2.40A/B/C/D1-244[»]
ProteinModelPortaliQ9KQH7.
SMRiQ9KQH7. Positions 2-244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC2021.

Proteomic databases

PRIDEiQ9KQH7.

Protocols and materials databases

DNASUi2613400.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF95169; AAF95169; VC_2021.
GeneIDi2613400.
KEGGivch:VC2021.
PATRICi20083074. VBIVibCho83274_1931.

Phylogenomic databases

eggNOGiCOG1028.
KOiK00059.
OMAiTGQELHV.
OrthoDBiEOG6N3CR8.

Enzyme and pathway databases

UniPathwayiUPA00094.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiFABG_VIBCH
AccessioniPrimary (citable) accession number: Q9KQH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: April 1, 2015
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.