Q9KQD8 (CHEB1_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	VC_2062

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Miscellaneous	In V.cholerae cheB2 is a pseudogene. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon HAMAP MF_00099		PRO_0000158036
Regions
	Domain	4 – 121	118	Response regulatory
	Domain	184 – 377	194	CheB-type methylesterase
Sites
	Active site	196	1	By similarity
	Active site	223	1	By similarity
	Active site	319	1	By similarity
Amino acid modifications
	Modified residue	55	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KQD8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 2D68E01FC90F510B	FASTA	377	40,379
	10 20 30 40 50 60 MAIKVLVVDD SSFFRRRVSE IINSESRLEV IDVAVNGKEA VEKAARLKPD VITMDIEMPV 70 80 90 100 110 120 MDGISAVREI MANNPVPILM FSSLTHDGAK ATLDALDAGA LDFLPKKFED IARNRDEAVT 130 140 150 160 170 180 LLQQRVLSIA SKKMFLRRPA APRPAPTTSI AASSSLSQER AAATSPLGNR PSTAVSAATR 190 200 210 220 230 240 FKASGKKYQL TAIGTSTGGP VALQKILTKL PVNYPHPIVL IQHMPATFTA AFASRLNSLC 250 260 270 280 290 300 KIEVKEAEDG DMLRPGVAYL APGGKQMMLD GRPGAARLRI IDGGDRMNYK PCVDVTFGSA 310 320 330 340 350 360 AKIFGDKVLS MILTGMGADG REGARMLKQA GATIWAQDEE SCVVYGMPQA VAKAGISTED 370 LPLERIAERM LVEVGLA

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF95208.1.
PIR	H82122.
RefSeq	NP_231694.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KQD8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2613442.
GenomeReviews	Gene locus VC_2062 in contig AE003852_GR.
KEGG	vch:VC2062.
PATRIC	20083158. VBIVibCho83274_1971.
TIGR	VC_2062.
Phylogenomic databases
HOGENOM	HBG705324.
OMA	KMAAELN.
PhylomeDB	Q9KQD8.
ProtClustDB	PRK00742.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_VIBCH

Accession

Primary (citable) accession number: Q9KQD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program