Q9KPY6 (PUR5_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	VC_2226

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1 › Vibrio cholerae O1 biovar El Tor

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine ribonucleotide biosynthetic process Inferred from sequence or structural similarity Ref.1. Source: TIGR
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from sequence or structural similarity Ref.1. Source: TIGR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 346

346

Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741

PRO_0000148270

Secondary structure

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346

Helix Strand Turn

Details...

Helix

19 – 33

15

Beta strand

57 – 66

10

Helix

70 – 77

8

Helix

84 – 97

14

Turn

98 – 100

3

Beta strand

102 – 114

13

Helix

117 – 134

18

Beta strand

137 – 145

9

Beta strand

155 – 166

12

Helix

167 – 169

3

Beta strand

181 – 186

6

Beta strand

188 – 190

3

Helix

195 – 205

11

Helix

218 – 223

6

Helix

230 – 239

10

Beta strand

244 – 247

4

Helix

252 – 256

5

Helix

257 – 260

4

Beta strand

265 – 270

6

Helix

278 – 287

10

Helix

291 – 295

5

Beta strand

302 – 308

7

Helix

310 – 322

13

Beta strand

327 – 335

9

Beta strand

338 – 340

3

Beta strand

342 – 346

5

Sequences

	Sequence		Length	Mass (Da)
	Q9KPY6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D440BA2570E5DC0D	FASTA	346	36,773
	10 20 30 40 50 60 MSGNNPSLSY KDAGVDIDAG NALVERIKGA VKRTRRPEVM GGLGGFGALC ELPTKYKHPV 70 80 90 100 110 120 LVSGTDGVGT KLRLALDMKK HDTIGIDLVA MCVNDLIVQG AEPLFFLDYY ATGKLDVDTA 130 140 150 160 170 180 AEVISGIADG CLQAGCALIG GETAEMPGMY EGEDYDVAGF CVGVVEKEEI IDGSKVQVGD 190 200 210 220 230 240 ALIAVGSSGP HSNGYSLVRK ILEVSKADKN ERLAGKTIGE HLLAPTKIYI KSGLKLIAEH 250 260 270 280 290 300 DIHAISHITG GGFWENIPRV LPEGTKAVID GKSWEWPVIF QWLQEKGNVT THEMYRTFNC 310 320 330 340 GVGLIIALPK DQANAAVALL QAEGETAWVI GEIAAANSNE AQVEIN

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE003852 Genomic DNA. Translation: AAF95370.1.

PIR

F82103.

RefSeq

NP_231857.1. NC_002505.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3P4E	X-ray	1.77	A	1-346	[»]

ProteinModelPortal

Q9KPY6.

SMR

Q9KPY6. Positions 7-343.

ModBase

Search...

Protein-protein interaction databases

STRING

243277.VC2226.

Protocols and materials databases

DNASU

2613266.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAF95370; AAF95370; VC_2226.

GeneID

2613266.

KEGG

vch:VC2226.

PATRIC

20083499. VBIVibCho83274_2124.

Phylogenomic databases

eggNOG

COG0150.

KO

K01933.

OMA

PRVLPKH.

ProtClustDB

PRK05385.

Enzyme and pathway databases

UniPathway

UPA00074; UER00129.

Family and domain databases

HAMAP

MF_00741_B. AIRS_B.

InterPro

IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]

Pfam

PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]

SUPFAM

SSF56042. AIR_synth_C. 1 hit.
SSF55326. PurM_N-like. 1 hit.

TIGRFAMs

TIGR00878. purM. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q9KPY6.

Entry information

Entry name

PUR5_VIBCH

Accession

Primary (citable) accession number: Q9KPY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program