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Protein

Phosphoribosylformylglycinamidine cyclo-ligase

Gene

purM

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase (purL)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphoribosylformylglycinamidine cyclo-ligase activity Source: TIGR

GO - Biological processi

  • 'de novo' IMP biosynthetic process Source: UniProtKB-HAMAP
  • purine ribonucleotide biosynthetic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciVCHO:VC2226-MONOMER.
UniPathwayiUPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligaseUniRule annotation (EC:6.3.3.1UniRule annotation)
Alternative name(s):
AIR synthaseUniRule annotation
AIRSUniRule annotation
Phosphoribosyl-aminoimidazole synthetaseUniRule annotation
Gene namesi
Name:purMUniRule annotation
Ordered Locus Names:VC_2226
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001482701 – 346Phosphoribosylformylglycinamidine cyclo-ligaseAdd BLAST346

Interactioni

Protein-protein interaction databases

STRINGi243277.VC2226.

Structurei

Secondary structure

1346
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi19 – 33Combined sources15
Beta strandi57 – 66Combined sources10
Helixi70 – 77Combined sources8
Helixi84 – 97Combined sources14
Turni98 – 100Combined sources3
Beta strandi102 – 114Combined sources13
Helixi117 – 134Combined sources18
Beta strandi137 – 145Combined sources9
Beta strandi155 – 166Combined sources12
Helixi167 – 169Combined sources3
Beta strandi181 – 186Combined sources6
Beta strandi188 – 190Combined sources3
Helixi195 – 205Combined sources11
Helixi218 – 223Combined sources6
Helixi230 – 239Combined sources10
Beta strandi244 – 247Combined sources4
Helixi252 – 256Combined sources5
Helixi257 – 260Combined sources4
Beta strandi265 – 270Combined sources6
Helixi278 – 287Combined sources10
Helixi291 – 295Combined sources5
Beta strandi302 – 308Combined sources7
Helixi310 – 322Combined sources13
Beta strandi327 – 335Combined sources9
Beta strandi338 – 340Combined sources3
Beta strandi342 – 346Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P4EX-ray1.77A1-346[»]
ProteinModelPortaliQ9KPY6.
SMRiQ9KPY6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KPY6.

Family & Domainsi

Sequence similaritiesi

Belongs to the AIR synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CXB. Bacteria.
COG0150. LUCA.
KOiK01933.
OMAiLNRHDTV.

Family and domain databases

CDDicd02196. PurM. 1 hit.
Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR016188. PurM-like_N.
IPR004733. PurM_cligase.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00878. purM. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KPY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGNNPSLSY KDAGVDIDAG NALVERIKGA VKRTRRPEVM GGLGGFGALC
60 70 80 90 100
ELPTKYKHPV LVSGTDGVGT KLRLALDMKK HDTIGIDLVA MCVNDLIVQG
110 120 130 140 150
AEPLFFLDYY ATGKLDVDTA AEVISGIADG CLQAGCALIG GETAEMPGMY
160 170 180 190 200
EGEDYDVAGF CVGVVEKEEI IDGSKVQVGD ALIAVGSSGP HSNGYSLVRK
210 220 230 240 250
ILEVSKADKN ERLAGKTIGE HLLAPTKIYI KSGLKLIAEH DIHAISHITG
260 270 280 290 300
GGFWENIPRV LPEGTKAVID GKSWEWPVIF QWLQEKGNVT THEMYRTFNC
310 320 330 340
GVGLIIALPK DQANAAVALL QAEGETAWVI GEIAAANSNE AQVEIN
Length:346
Mass (Da):36,773
Last modified:October 1, 2000 - v1
Checksum:iD440BA2570E5DC0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95370.1.
PIRiF82103.
RefSeqiNP_231857.1. NC_002505.1.
WP_000016423.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95370; AAF95370; VC_2226.
GeneIDi2613266.
KEGGivch:VC2226.
PATRICi20083499. VBIVibCho83274_2124.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95370.1.
PIRiF82103.
RefSeqiNP_231857.1. NC_002505.1.
WP_000016423.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P4EX-ray1.77A1-346[»]
ProteinModelPortaliQ9KPY6.
SMRiQ9KPY6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC2226.

Protocols and materials databases

DNASUi2613266.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF95370; AAF95370; VC_2226.
GeneIDi2613266.
KEGGivch:VC2226.
PATRICi20083499. VBIVibCho83274_2124.

Phylogenomic databases

eggNOGiENOG4105CXB. Bacteria.
COG0150. LUCA.
KOiK01933.
OMAiLNRHDTV.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00129.
BioCyciVCHO:VC2226-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9KPY6.

Family and domain databases

CDDicd02196. PurM. 1 hit.
Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR016188. PurM-like_N.
IPR004733. PurM_cligase.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00878. purM. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPUR5_VIBCH
AccessioniPrimary (citable) accession number: Q9KPY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.