Phosphoheptose isomerase - Vibrio cholerae

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Q9KPY2 (GMHA_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 59. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphoheptose isomerase
EC=5.3.1.28

Alternative name(s):
Sedoheptulose 7-phosphate isomerase

Gene names

Name:	gmhA
Ordered Locus Names:	VC_2230

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate By similarity. HAMAP MF_00067
Catalytic activity	D-sedoheptulose 7-phosphate = D-glycero-D-manno-heptose 7-phosphate. HAMAP MF_00067
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00067
Pathway	Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7-phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1. HAMAP MF_00067 Bacterial outer membrane biogenesis; LPS core biosynthesis. HAMAP MF_00067
Subunit structure	Homotetramer By similarity. HAMAP MF_00067
Subcellular location	Cytoplasm By similarity HAMAP MF_00067.
Miscellaneous	The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate By similarity. HAMAP MF_00067
Sequence similarities	Belongs to the SIS family. GmhA subfamily. Contains 1 SIS domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide core region biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	D-sedoheptulose 7-phosphate isomerase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 191

191

Phosphoheptose isomerase HAMAP MF_00067

PRO_0000136548

Regions

Domain

37 – 191

155

SIS

Region

52 – 54

Substrate binding By similarity

Region

93 – 94

Substrate binding By similarity

Region

119 – 121

Substrate binding By similarity

Sites

Metal binding

Zinc By similarity

Metal binding

Zinc By similarity

Metal binding

172

Zinc By similarity

Metal binding

180

Zinc By similarity

Binding site

Substrate By similarity

Binding site

124

Substrate By similarity

Binding site

172

Substrate By similarity

Secondary structure

191

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q9KPY2 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: B5A57D89F437FED0
Show »

FASTA

191

20,646

        10         20         30         40         50         60 
MYQDLIRSEL TEAADVLQKF LSDDHNIAQI EAAAKLIADS FKQGGKVLSC GNGGSHCDAM 

        70         80         90        100        110        120 
HFAEELTGRY RENRPGYPGI AISDPSHLSC VSNDFGYDYV FSRYVEAVGA KGDVLFGLST 

       130        140        150        160        170        180 
SGNSGNILKA IEAAKAKGMK TIALTGKDGG KMAGLADVEI RVPHFGYADR IQEVHIKIIH 

       190 
IIIQLIEKEM A

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.
[2]	"Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides." Valvano M.A., Messner P., Kosma P. Microbiology 148:1979-1989(2002) [PubMed: 12101286] [Abstract] Cited for: BIOSYNTHESIS OF NUCLEOTIDE-ACTIVATED GLYCERO-MANNO-HEPTOSE.
[3]	"Crystal structures of two putative phosphoheptose isomerases." Seetharaman J., Rajashankar K.R., Solorzano V., Kniewel R., Lima C.D., Bonanno J.B., Burley S.K., Swaminathan S. Proteins 63:1092-1096(2006) [PubMed: 16477602] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE003852 Genomic DNA. Translation: AAF95374.1.

PIR

B82104.

RefSeq

NP_231861.1. NC_002505.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1X94	X-ray	2.50	A/B	1-191	[»]

ProteinModelPortal

Q9KPY2.

SMR

Q9KPY2. Positions 1-190.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2613270.

GenomeReviews

Gene locus VC_2230 in contig AE003852_GR.

KEGG

vch:VC2230.

PATRIC

20083505. VBIVibCho83274_2127.

TIGR

VC_2230.

Phylogenomic databases

HOGENOM

HBG671955.

OMA

ANDLGYD.

PhylomeDB

Q9KPY2.

ProtClustDB

PRK00414.

Family and domain databases

HAMAP

MF_00067. GmhA.
[Tree]

InterPro

IPR004515. Phosphoheptose_Isoase_subgr.
IPR020620. Phosphoheptose_isomerase.
IPR001347. SIS.
[Graphical view]

K03271.

Pfam

PF01380. SIS. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00441. GmhA. 1 hit.

PROSITE

PS51464. SIS. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GMHA_VIBCH

Accession

Primary (citable) accession number: Q9KPY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents