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Protein

Phosphoheptose isomerase

Gene

gmhA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.By similarity

Catalytic activityi

D-sedoheptulose 7-phosphate = D-glycero-D-manno-heptose 7-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: D-glycero-D-manno-heptose 7-phosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Phosphoheptose isomerase (gmhA), Phosphoheptose isomerase (gmhA)
This subpathway is part of the pathway D-glycero-D-manno-heptose 7-phosphate biosynthesis, which is itself part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate, the pathway D-glycero-D-manno-heptose 7-phosphate biosynthesis and in Carbohydrate biosynthesis.

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611ZincBy similarity
Metal bindingi65 – 651ZincBy similarity
Binding sitei65 – 651SubstrateBy similarity
Binding sitei124 – 1241SubstrateBy similarity
Metal bindingi172 – 1721ZincBy similarity
Binding sitei172 – 1721SubstrateBy similarity
Metal bindingi180 – 1801ZincBy similarity

GO - Molecular functioni

  • carbohydrate binding Source: InterPro
  • D-sedoheptulose 7-phosphate isomerase activity Source: TIGR
  • zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism, Lipopolysaccharide biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciVCHO:VC2230-MONOMER.
BRENDAi5.3.1.28. 6626.
UniPathwayiUPA00041; UER00436.
UPA00958.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoheptose isomerase (EC:5.3.1.28)
Alternative name(s):
Sedoheptulose 7-phosphate isomerase
Gene namesi
Name:gmhA
Ordered Locus Names:VC_2230
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 191191Phosphoheptose isomerasePRO_0000136548Add
BLAST

Proteomic databases

PRIDEiQ9KPY2.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi243277.VC2230.

Structurei

Secondary structure

1
191
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 2119Combined sources
Helixi24 – 4118Combined sources
Turni42 – 443Combined sources
Beta strandi47 – 504Combined sources
Beta strandi52 – 543Combined sources
Helixi55 – 7016Combined sources
Beta strandi78 – 814Combined sources
Helixi100 – 1089Combined sources
Beta strandi114 – 1229Combined sources
Helixi125 – 13713Combined sources
Beta strandi140 – 1467Combined sources
Helixi150 – 1523Combined sources
Turni153 – 1553Combined sources
Beta strandi156 – 1638Combined sources
Helixi168 – 18922Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X94X-ray2.50A/B1-191[»]
ProteinModelPortaliQ9KPY2.
SMRiQ9KPY2. Positions 1-190.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KPY2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 191155SISAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni52 – 543Substrate bindingBy similarity
Regioni93 – 942Substrate bindingBy similarity
Regioni119 – 1213Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the SIS family. GmhA subfamily.Curated
Contains 1 SIS domain.Curated

Phylogenomic databases

eggNOGiENOG4105F55. Bacteria.
COG0279. LUCA.
KOiK03271.
OMAiEMHILMI.

Family and domain databases

HAMAPiMF_00067. GmhA. 1 hit.
InterProiIPR004515. Phosphoheptose_Isoase.
IPR001347. SIS.
[Graphical view]
PfamiPF13580. SIS_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00441. gmhA. 1 hit.
PROSITEiPS51464. SIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KPY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYQDLIRSEL TEAADVLQKF LSDDHNIAQI EAAAKLIADS FKQGGKVLSC
60 70 80 90 100
GNGGSHCDAM HFAEELTGRY RENRPGYPGI AISDPSHLSC VSNDFGYDYV
110 120 130 140 150
FSRYVEAVGA KGDVLFGLST SGNSGNILKA IEAAKAKGMK TIALTGKDGG
160 170 180 190
KMAGLADVEI RVPHFGYADR IQEVHIKIIH IIIQLIEKEM A
Length:191
Mass (Da):20,646
Last modified:October 1, 2000 - v1
Checksum:iB5A57D89F437FED0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95374.1.
PIRiB82104.
RefSeqiNP_231861.1. NC_002505.1.
WP_000284054.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95374; AAF95374; VC_2230.
GeneIDi2613270.
KEGGivch:VC2230.
PATRICi20083505. VBIVibCho83274_2127.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95374.1.
PIRiB82104.
RefSeqiNP_231861.1. NC_002505.1.
WP_000284054.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X94X-ray2.50A/B1-191[»]
ProteinModelPortaliQ9KPY2.
SMRiQ9KPY2. Positions 1-190.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC2230.

Proteomic databases

PRIDEiQ9KPY2.

Protocols and materials databases

DNASUi2613270.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF95374; AAF95374; VC_2230.
GeneIDi2613270.
KEGGivch:VC2230.
PATRICi20083505. VBIVibCho83274_2127.

Phylogenomic databases

eggNOGiENOG4105F55. Bacteria.
COG0279. LUCA.
KOiK03271.
OMAiEMHILMI.

Enzyme and pathway databases

UniPathwayiUPA00041; UER00436.
UPA00958.
BioCyciVCHO:VC2230-MONOMER.
BRENDAi5.3.1.28. 6626.

Miscellaneous databases

EvolutionaryTraceiQ9KPY2.

Family and domain databases

HAMAPiMF_00067. GmhA. 1 hit.
InterProiIPR004515. Phosphoheptose_Isoase.
IPR001347. SIS.
[Graphical view]
PfamiPF13580. SIS_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00441. gmhA. 1 hit.
PROSITEiPS51464. SIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGMHA_VIBCH
AccessioniPrimary (citable) accession number: Q9KPY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate.By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.