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Q9KPT8 (PROB_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamate 5-kinase
EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase
Short name=GK
Gene names
Name:proB
Ordered Locus Names:VC_2274
OrganismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]
Taxonomic identifier243277 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio ›

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Contains 1 PUA domain.

Sequence caution

The sequence AAF95418.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 377377Glutamate 5-kinase HAMAP-Rule MF_00456
PRO_0000109751

Regions

Domain283 – 36179PUA
Nucleotide binding176 – 1772ATP By similarity
Nucleotide binding218 – 2247ATP By similarity

Sites

Binding site171ATP By similarity
Binding site571Substrate By similarity
Binding site1441Substrate By similarity
Binding site1561Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KPT8 [UniParc].

Last modified September 26, 2001. Version 2.
Checksum: 080D398A5432DF61

FASTA37740,151
        10         20         30         40         50         60 
MTTNQRSIKA PQTVVVKLGT SVLTGGTLAL DRAHMVELAR QCAELKKQGH SVVIVTSGAI 

        70         80         90        100        110        120 
AAGREHLGYP ALPNAISSKQ LLAAVGQSRL IQVWESLFAI YGIKIGQMLL TRADLDDRER 

       130        140        150        160        170        180 
FLNARDTINA LVENGIVPVV NENDAVATNE IKVGDNDNLS ALVGILCGAD KLLLLTDQKG 

       190        200        210        220        230        240 
LFTADPRKDP NAQLIKEVTT IDDTLRKIAG GSGTTLGTGG MATKLQAADI ARRAGIEVII 

       250        260        270        280        290        300 
AAGRAPNVIF DSLSDEPQGT RFLALEEALE NRKRWILAGP AASGDIVIDD GAVKAVQTKG 

       310        320        330        340        350        360 
SSLLAKGVVR VQGQFARGEV VRVLDKQSHL IARGIAAYSN DELAQIAGKH SKEIIDILGY 

       370 
DHGSEVIHRD DMVVIQE

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE003852 Genomic DNA. Translation: AAF95418.1. Different initiation.
PIRF82095.
RefSeqNP_231905.2. NC_002505.1.

3D structure databases

ProteinModelPortalQ9KPT8.
SMRQ9KPT8. Positions 12-373.
ModBaseSearch...

Protein-protein interaction databases

STRING243277.VC2274.

Protocols and materials databases

DNASU2613196.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF95418; AAF95418; VC_2274.
GeneID2613196.
KEGGvch:VC2274.
PATRIC20083589. VBIVibCho83274_2169.

Phylogenomic databases

eggNOGCOG0263.
KOK00931.
OMADHLQLRG.
ProtClustDBPRK05429.

Enzyme and pathway databases

UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF53633. Aa_kinase. 1 hit.
SSF88697. PUA-like. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB_VIBCH
AccessionPrimary (citable) accession number: Q9KPT8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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