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Protein

Na(+)-translocating NADH-quinone reductase subunit B

Gene

nqrB

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na+ ions from the cytoplasm to the periplasm. NqrA to NqrE are probably involved in the second step, the conversion of ubisemiquinone to ubiquinol.UniRule annotation

Catalytic activityi

NADH + ubiquinone + n Na+(In) = NAD+ + ubiquinol + n Na+(Out).UniRule annotation

Cofactori

FMNUniRule annotation

GO - Molecular functioni

  • FMN binding Source: InterPro
  • NADH dehydrogenase (ubiquinone) activity Source: TIGR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Flavoprotein, FMN, NAD, Sodium, Ubiquinone

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16198.
BRENDAi1.6.5.8. 6626.

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)-translocating NADH-quinone reductase subunit BUniRule annotation (EC:1.6.5.8UniRule annotation)
Short name:
Na(+)-NQR subunit BUniRule annotation
Short name:
Na(+)-translocating NQR subunit BUniRule annotation
Alternative name(s):
NQR complex subunit BUniRule annotation
NQR-1 subunit BUniRule annotation
Gene namesi
Name:nqrBUniRule annotation
Ordered Locus Names:VC_2294
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei23 – 40HelicalUniRule annotationAdd BLAST18
Transmembranei56 – 76HelicalUniRule annotationAdd BLAST21
Transmembranei129 – 149HelicalUniRule annotationAdd BLAST21
Transmembranei164 – 184HelicalUniRule annotationAdd BLAST21
Transmembranei275 – 295HelicalUniRule annotationAdd BLAST21
Transmembranei297 – 317HelicalUniRule annotationAdd BLAST21
Transmembranei325 – 345HelicalUniRule annotationAdd BLAST21
Transmembranei358 – 378HelicalUniRule annotationAdd BLAST21
Transmembranei381 – 401HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000744441 – 415Na(+)-translocating NADH-quinone reductase subunit BAdd BLAST415

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei236FMN phosphoryl threonineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and NqrF.UniRule annotation

Protein-protein interaction databases

DIPiDIP-61338N.
STRINGi243277.VC2294.

Structurei

Secondary structure

1415
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi54 – 61Combined sources8
Turni62 – 64Combined sources3
Helixi65 – 84Combined sources20
Turni89 – 92Combined sources4
Helixi93 – 96Combined sources4
Helixi100 – 108Combined sources9
Helixi122 – 150Combined sources29
Helixi159 – 168Combined sources10
Helixi177 – 187Combined sources11
Helixi188 – 192Combined sources5
Beta strandi196 – 199Combined sources4
Helixi204 – 215Combined sources12
Helixi217 – 219Combined sources3
Beta strandi221 – 224Combined sources4
Beta strandi226 – 230Combined sources5
Turni237 – 243Combined sources7
Helixi272 – 288Combined sources17
Beta strandi291 – 293Combined sources3
Helixi295 – 314Combined sources20
Beta strandi315 – 317Combined sources3
Helixi322 – 324Combined sources3
Helixi336 – 342Combined sources7
Helixi354 – 373Combined sources20
Helixi383 – 404Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4P6VX-ray3.50B1-415[»]
SMRiQ9KPS2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NqrB/RnfD family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108J5C. Bacteria.
COG1805. LUCA.
KOiK00347.
OMAiFNPAYPE.

Family and domain databases

HAMAPiMF_00426. NqrB. 1 hit.
InterProiIPR004338. NADH_Q_Rdtase_NQR2_RnfD.
IPR010966. NqrB.
[Graphical view]
PANTHERiPTHR30578:SF1. PTHR30578:SF1. 1 hit.
PfamiPF03116. NQR2_RnfD_RnfE. 1 hit.
[Graphical view]
PIRSFiPIRSF016055. NADH-UbQ_OxRdtase_B_su. 1 hit.
TIGRFAMsiTIGR01937. nqrB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KPS2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLKKFLEDI EHHFEPGGKH EKWFALYEAA ATLFYTPGLV TKRSSHVRDS
60 70 80 90 100
VDLKRIMIMV WLAVFPAMFW GMYNAGGQAI AALNHLYSGD QLAAIVAGNW
110 120 130 140 150
HYWLTEMLGG TMSSDAGWGS KMLLGATYFL PIYATVFIVG GFWEVLFCMV
160 170 180 190 200
RKHEVNEGFF VTSILFALIV PPTLPLWQAA LGITFGVVVA KEVFGGTGRN
210 220 230 240 250
FLNPALAGRA FLFFAYPAQI SGDLVWTAAD GYSGATALSQ WAQGGAGALI
260 270 280 290 300
NNATGQTITW MDAFIGNIPG SIGEVSTLAL MIGAAFIVYM GIASWRIIGG
310 320 330 340 350
VMIGMILLST LFNVIGSDTN AMFNMPWHWH LVLGGFAFGM FFMATDPVSA
360 370 380 390 400
SFTNSGKWAY GILIGVMCVL IRVVNPAYPE GMMLAILFAN LFAPLFDHVV
410
VERNIKRRLA RYGKQ
Length:415
Mass (Da):45,357
Last modified:August 14, 2001 - v2
Checksum:i8DF4F1FD6DAE8DAF
GO

Sequence cautioni

The sequence AAF95438 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117331 Genomic DNA. Translation: AAD29963.1.
AE003852 Genomic DNA. Translation: AAF95438.1. Different initiation.
PIRiF82094.
RefSeqiNP_231925.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95438; AAF95438; VC_2294.
GeneIDi2613216.
KEGGivch:VC2294.
PATRICi20083627. VBIVibCho83274_2188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117331 Genomic DNA. Translation: AAD29963.1.
AE003852 Genomic DNA. Translation: AAF95438.1. Different initiation.
PIRiF82094.
RefSeqiNP_231925.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4P6VX-ray3.50B1-415[»]
SMRiQ9KPS2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61338N.
STRINGi243277.VC2294.

Protocols and materials databases

DNASUi2613216.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF95438; AAF95438; VC_2294.
GeneIDi2613216.
KEGGivch:VC2294.
PATRICi20083627. VBIVibCho83274_2188.

Phylogenomic databases

eggNOGiENOG4108J5C. Bacteria.
COG1805. LUCA.
KOiK00347.
OMAiFNPAYPE.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16198.
BRENDAi1.6.5.8. 6626.

Family and domain databases

HAMAPiMF_00426. NqrB. 1 hit.
InterProiIPR004338. NADH_Q_Rdtase_NQR2_RnfD.
IPR010966. NqrB.
[Graphical view]
PANTHERiPTHR30578:SF1. PTHR30578:SF1. 1 hit.
PfamiPF03116. NQR2_RnfD_RnfE. 1 hit.
[Graphical view]
PIRSFiPIRSF016055. NADH-UbQ_OxRdtase_B_su. 1 hit.
TIGRFAMsiTIGR01937. nqrB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNQRB_VIBCH
AccessioniPrimary (citable) accession number: Q9KPS2
Secondary accession number(s): Q9X4Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: August 14, 2001
Last modified: November 2, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.