Na(+)-translocating NADH-quinone reductase subunit B - Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

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Q9KPS2 (NQRB_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Na(+)-translocating NADH-quinone reductase subunit B
Short name=Na(+)-NQR subunit B
Short name=Na(+)-translocating NQR subunit B
EC=1.6.5.-

Alternative name(s):
NQR complex subunit B
NQR-1 subunit B

Gene names

Name:	nqrB
Ordered Locus Names:	VC_2294

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio ›

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na⁺ ions from the cytoplasm to the periplasm. NqrA to NqrE are probably involved in the second step, the conversion of ubisemiquinone to ubiquinol. HAMAP-Rule MF_00426
Catalytic activity	NADH + ubiquinone + Na⁺(In) = NAD⁺ + ubiquinol + Na⁺(Out). HAMAP-Rule MF_00426
Cofactor	FMN By similarity.
Subunit structure	Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and NqrF By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential HAMAP-Rule MF_00426.
Sequence similarities	Belongs to the NqrB/RnfD family.
Sequence caution	The sequence AAF95438.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ion transport Sodium transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	FMN Flavoprotein NAD Sodium Ubiquinone
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	respiratory electron transport chain Inferred from electronic annotation. Source: InterPro sodium ion transport Inferred from electronic annotation. Source: HAMAP
Cellular_component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	FMN binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from sequence or structural similarity Ref.2. Source: TIGR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 415

415

Na(+)-translocating NADH-quinone reductase subunit B HAMAP-Rule MF_00426

PRO_0000074444

Regions

Transmembrane

23 – 40

Helical; Potential

Transmembrane

60 – 82

Helical; Potential

Transmembrane

125 – 147

Helical; Potential

Transmembrane

159 – 181

Helical; Potential

Transmembrane

270 – 292

Helical; Potential

Transmembrane

297 – 316

Helical; Potential

Transmembrane

326 – 345

Helical; Potential

Transmembrane

358 – 375

Helical; Potential

Transmembrane

379 – 401

Helical; Potential

Amino acid modifications

Modified residue

236

FMN phosphoryl threonine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9KPS2 [UniParc].

Last modified August 14, 2001. Version 2.
Checksum: 8DF4F1FD6DAE8DAF
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FASTA

415

45,357

        10         20         30         40         50         60 
MGLKKFLEDI EHHFEPGGKH EKWFALYEAA ATLFYTPGLV TKRSSHVRDS VDLKRIMIMV 

        70         80         90        100        110        120 
WLAVFPAMFW GMYNAGGQAI AALNHLYSGD QLAAIVAGNW HYWLTEMLGG TMSSDAGWGS 

       130        140        150        160        170        180 
KMLLGATYFL PIYATVFIVG GFWEVLFCMV RKHEVNEGFF VTSILFALIV PPTLPLWQAA 

       190        200        210        220        230        240 
LGITFGVVVA KEVFGGTGRN FLNPALAGRA FLFFAYPAQI SGDLVWTAAD GYSGATALSQ 

       250        260        270        280        290        300 
WAQGGAGALI NNATGQTITW MDAFIGNIPG SIGEVSTLAL MIGAAFIVYM GIASWRIIGG 

       310        320        330        340        350        360 
VMIGMILLST LFNVIGSDTN AMFNMPWHWH LVLGGFAFGM FFMATDPVSA SFTNSGKWAY 

       370        380        390        400        410 
GILIGVMCVL IRVVNPAYPE GMMLAILFAN LFAPLFDHVV VERNIKRRLA RYGKQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Effects of changes in membrane sodium flux on virulence gene expression in Vibrio cholerae." Haese C.C., Mekalanos J.J. Proc. Natl. Acad. Sci. U.S.A. 96:3183-3187(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.
[2]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF117331 Genomic DNA. Translation: AAD29963.1. AE003852 Genomic DNA. Translation: AAF95438.1. Different initiation.
PIR	F82094.
RefSeq	NP_231925.1. NC_002505.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	243277.VC2294.
Protocols and materials databases
DNASU	2613216.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF95438; AAF95438; VC_2294.
GeneID	2613216.
KEGG	vch:VC2294.
PATRIC	20083627. VBIVibCho83274_2188.
Phylogenomic databases
eggNOG	COG1805.
KO	K00347.
OMA	TVFYTPG.
ProtClustDB	PRK05349.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16198.
Family and domain databases
HAMAP	MF_00426. NqrB.
InterPro	IPR010966. NADH-UbQ_OxRdtase_suB. IPR004338. NADH_Q_Rdtase_NQR2_RnfD. [Graphical view]
Pfam	PF03116. NQR2_RnfD_RnfE. 1 hit. [Graphical view]
PIRSF	PIRSF016055. NADH-UbQ_OxRdtase_B_su. 1 hit.
TIGRFAMs	TIGR01937. nqrB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NQRB_VIBCH

Accession

Primary (citable) accession number: Q9KPS2
Secondary accession number(s): Q9X4Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	August 14, 2001
Last modified:	May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents