Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9KPS2

- NQRB_VIBCH

UniProt

Q9KPS2 - NQRB_VIBCH

Protein

Na(+)-translocating NADH-quinone reductase subunit B

Gene

nqrB

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (14 Aug 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na+ ions from the cytoplasm to the periplasm. NqrA to NqrE are probably involved in the second step, the conversion of ubisemiquinone to ubiquinol.

    Catalytic activityi

    NADH + ubiquinone + Na+(In) = NAD+ + ubiquinol + Na+(Out).

    Cofactori

    FMN.By similarity

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. NADH dehydrogenase (ubiquinone) activity Source: TIGR

    GO - Biological processi

    1. respiratory electron transport chain Source: InterPro
    2. sodium ion transport Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Ion transport, Sodium transport, Transport

    Keywords - Ligandi

    Flavoprotein, FMN, NAD, Sodium, Ubiquinone

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16198.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Na(+)-translocating NADH-quinone reductase subunit B (EC:1.6.5.-)
    Short name:
    Na(+)-NQR subunit B
    Short name:
    Na(+)-translocating NQR subunit B
    Alternative name(s):
    NQR complex subunit B
    NQR-1 subunit B
    Gene namesi
    Name:nqrB
    Ordered Locus Names:VC_2294
    OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
    Taxonomic identifieri243277 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000000584: Chromosome 1

    Subcellular locationi

    GO - Cellular componenti

    1. Gram-negative-bacterium-type cell wall Source: InterPro
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 415415Na(+)-translocating NADH-quinone reductase subunit BPRO_0000074444Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei236 – 2361FMN phosphoryl threonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and NqrF.By similarity

    Protein-protein interaction databases

    STRINGi243277.VC2294.

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei23 – 4018HelicalSequence AnalysisAdd
    BLAST
    Transmembranei60 – 8223HelicalSequence AnalysisAdd
    BLAST
    Transmembranei125 – 14723HelicalSequence AnalysisAdd
    BLAST
    Transmembranei159 – 18123HelicalSequence AnalysisAdd
    BLAST
    Transmembranei270 – 29223HelicalSequence AnalysisAdd
    BLAST
    Transmembranei297 – 31620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei326 – 34520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei358 – 37518HelicalSequence AnalysisAdd
    BLAST
    Transmembranei379 – 40123HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NqrB/RnfD family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1805.
    KOiK00347.
    OMAiTVFYTPG.
    OrthoDBiEOG65BDMX.

    Family and domain databases

    HAMAPiMF_00426. NqrB.
    InterProiIPR010966. NADH-UbQ_OxRdtase_suB.
    IPR004338. NADH_Q_Rdtase_NQR2_RnfD.
    [Graphical view]
    PfamiPF03116. NQR2_RnfD_RnfE. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016055. NADH-UbQ_OxRdtase_B_su. 1 hit.
    TIGRFAMsiTIGR01937. nqrB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9KPS2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLKKFLEDI EHHFEPGGKH EKWFALYEAA ATLFYTPGLV TKRSSHVRDS    50
    VDLKRIMIMV WLAVFPAMFW GMYNAGGQAI AALNHLYSGD QLAAIVAGNW 100
    HYWLTEMLGG TMSSDAGWGS KMLLGATYFL PIYATVFIVG GFWEVLFCMV 150
    RKHEVNEGFF VTSILFALIV PPTLPLWQAA LGITFGVVVA KEVFGGTGRN 200
    FLNPALAGRA FLFFAYPAQI SGDLVWTAAD GYSGATALSQ WAQGGAGALI 250
    NNATGQTITW MDAFIGNIPG SIGEVSTLAL MIGAAFIVYM GIASWRIIGG 300
    VMIGMILLST LFNVIGSDTN AMFNMPWHWH LVLGGFAFGM FFMATDPVSA 350
    SFTNSGKWAY GILIGVMCVL IRVVNPAYPE GMMLAILFAN LFAPLFDHVV 400
    VERNIKRRLA RYGKQ 415
    Length:415
    Mass (Da):45,357
    Last modified:August 14, 2001 - v2
    Checksum:i8DF4F1FD6DAE8DAF
    GO

    Sequence cautioni

    The sequence AAF95438.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF117331 Genomic DNA. Translation: AAD29963.1.
    AE003852 Genomic DNA. Translation: AAF95438.1. Different initiation.
    PIRiF82094.
    RefSeqiNP_231925.1. NC_002505.1.

    Genome annotation databases

    EnsemblBacteriaiAAF95438; AAF95438; VC_2294.
    GeneIDi2613216.
    KEGGivch:VC2294.
    PATRICi20083627. VBIVibCho83274_2188.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF117331 Genomic DNA. Translation: AAD29963.1 .
    AE003852 Genomic DNA. Translation: AAF95438.1 . Different initiation.
    PIRi F82094.
    RefSeqi NP_231925.1. NC_002505.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243277.VC2294.

    Protocols and materials databases

    DNASUi 2613216.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF95438 ; AAF95438 ; VC_2294 .
    GeneIDi 2613216.
    KEGGi vch:VC2294.
    PATRICi 20083627. VBIVibCho83274_2188.

    Phylogenomic databases

    eggNOGi COG1805.
    KOi K00347.
    OMAi TVFYTPG.
    OrthoDBi EOG65BDMX.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16198.

    Family and domain databases

    HAMAPi MF_00426. NqrB.
    InterProi IPR010966. NADH-UbQ_OxRdtase_suB.
    IPR004338. NADH_Q_Rdtase_NQR2_RnfD.
    [Graphical view ]
    Pfami PF03116. NQR2_RnfD_RnfE. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016055. NADH-UbQ_OxRdtase_B_su. 1 hit.
    TIGRFAMsi TIGR01937. nqrB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Effects of changes in membrane sodium flux on virulence gene expression in Vibrio cholerae."
      Haese C.C., Mekalanos J.J.
      Proc. Natl. Acad. Sci. U.S.A. 96:3183-3187(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 39315 / El Tor Inaba N16961.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39315 / El Tor Inaba N16961.

    Entry informationi

    Entry nameiNQRB_VIBCH
    AccessioniPrimary (citable) accession number: Q9KPS2
    Secondary accession number(s): Q9X4Q4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 14, 2001
    Last sequence update: August 14, 2001
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3