Reviewed, UniProtKB/Swiss-Prot Q9KPR9 (RLMG_VIBCH)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	VC_2297

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366530

Sequences

	Sequence		Length	Mass (Da)
	Q9KPR9-1 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: FB166ECA68CBA65F	FASTA	376	42,051
	10 20 30 40 50 60 MKTELTLLEQ TLTLHRFPKR NNETLQAWDA GDEYLIQHVE QLALPESSHI VIINDHFGTL 70 80 90 100 110 120 SCWFSQKHKV SMMSDSYIAH QATQANLQQN QRPPVQLLTT LDPVPNDASV VLLQLPKSNR 130 140 150 160 170 180 HLVWILSQLR KALSPNIPII AVNKAKEIHT STLNLFEKHL GPTTTSLAWK KHRLVFSSAT 190 200 210 220 230 240 VNPANEVNPE CGWSIEPYAI TLTNLPNVYS GESLDLGSRF ILEHLPADPT LEDFIDLGCG 250 260 270 280 290 300 NGVLSVRLGQ LNPQAKITCV DESFMAIASA QKNLHDNLGK RDIHCIANNC LDGFPAHSSS 310 320 330 340 350 360 MIVCNPPFHQ QQTITDHIAW QMFCDSKHVL KKGGKLWVIG NRHLGYDVKL ARLFGKSHVR 370 VIANNSKFVI LQAIKS

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF95441.1. Different initiation.
PIR	A82095.
RefSeq	NP_231928.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2613093.
GenomeReviews	Gene locus VC_2297 in contig AE003852_GR.
KEGG	vch:VC2297.
TIGR	VC_2297.
Phylogenomic databases
HOGENOM	HBG321931.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_VIBCH

Accession

Primary (citable) accession number: Q9KPR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	January 19, 2010
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)