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Protein

2-dehydropantoate 2-reductase

Gene

panE

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.

Catalytic activityi

(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)
  2. 2-dehydropantoate 2-reductase (panE)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31NADP; via amide nitrogenBy similarity1
Binding sitei94NADP; via amide nitrogenBy similarity1
Binding sitei94SubstrateBy similarity1
Binding sitei120NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Active sitei175Proton donorBy similarity1
Binding sitei179SubstrateBy similarity1
Binding sitei183SubstrateBy similarity1
Binding sitei193SubstrateBy similarity1
Binding sitei242SubstrateBy similarity1
Binding sitei245SubstrateBy similarity1
Binding sitei257NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 12NADPBy similarity6

GO - Molecular functioni

  • 2-dehydropantoate 2-reductase activity Source: TIGR
  • NADP binding Source: GO_Central

GO - Biological processi

  • pantothenate biosynthetic process Source: TIGR
  • pantothenate biosynthetic process from valine Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pantothenate biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciVCHO:VC2307-MONOMER.
UniPathwayiUPA00028; UER00004.

Names & Taxonomyi

Protein namesi
Recommended name:
2-dehydropantoate 2-reductase (EC:1.1.1.169)
Alternative name(s):
Ketopantoate reductase
Short name:
KPA reductase
Short name:
KPR
Gene namesi
Name:panE
Ordered Locus Names:VC_2307
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001573061 – 2962-dehydropantoate 2-reductaseAdd BLAST296

Interactioni

Protein-protein interaction databases

STRINGi243277.VC2307.

Structurei

3D structure databases

ProteinModelPortaliQ9KPQ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ketopantoate reductase family.Curated

Phylogenomic databases

eggNOGiENOG4108JZF. Bacteria.
COG1893. LUCA.
KOiK00077.
OMAiRIPQPYC.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR003710. ApbA.
IPR013752. KPA_reductase.
IPR013332. KPR_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02558. ApbA. 1 hit.
PF08546. ApbA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00745. apbA_panE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KPQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIVVLGPGA VGSLWALHLH SAGHQVALWS RQAQPTITLQ LDEEAPISFR
60 70 80 90 100
NQNLDTLIHA DLLLITVKAW QVEAALQPLL PHLNRETILL FMHNGMGAVE
110 120 130 140 150
AISESLTHFP VLFATTTHGA LKATLNQVSH TGFGQTQVGP FNALGARCDF
160 170 180 190 200
IADVFNHALA PVTWNPEIQQ ALWRKLAVNC AINPLTAIHQ CANGALVAPE
210 220 230 240 250
FTPIITAILD EVTAVMQAEA ISGEAEALRD GVYQVIQATA ANLSSMHQDV
260 270 280 290
FHRRPTEIDF ITGYVVRKGE QHGIATPVNS ALYQQIKTLE QSWSKA
Length:296
Mass (Da):32,201
Last modified:October 1, 2000 - v1
Checksum:iA594D8EAFEE5EEAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95451.1.
PIRiD82092.
RefSeqiNP_231938.1. NC_002505.1.
WP_001028571.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95451; AAF95451; VC_2307.
GeneIDi2613103.
KEGGivch:VC2307.
PATRICi20083649. VBIVibCho83274_2199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95451.1.
PIRiD82092.
RefSeqiNP_231938.1. NC_002505.1.
WP_001028571.1. NC_002505.1.

3D structure databases

ProteinModelPortaliQ9KPQ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC2307.

Protocols and materials databases

DNASUi2613103.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF95451; AAF95451; VC_2307.
GeneIDi2613103.
KEGGivch:VC2307.
PATRICi20083649. VBIVibCho83274_2199.

Phylogenomic databases

eggNOGiENOG4108JZF. Bacteria.
COG1893. LUCA.
KOiK00077.
OMAiRIPQPYC.

Enzyme and pathway databases

UniPathwayiUPA00028; UER00004.
BioCyciVCHO:VC2307-MONOMER.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR003710. ApbA.
IPR013752. KPA_reductase.
IPR013332. KPR_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02558. ApbA. 1 hit.
PF08546. ApbA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00745. apbA_panE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPANE_VIBCH
AccessioniPrimary (citable) accession number: Q9KPQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2002
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.