Reviewed, UniProtKB/Swiss-Prot Q9KNW4 (ASTD_VIBCH)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	VC_2616

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_0000262431
Regions
	Nucleotide binding	220 – 225	6	NAD By similarity
Sites
	Active site	243	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KNW4-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 07E9FF9E24C091F2	FASTA	485	52,193
	10 20 30 40 50 60 MTHWIAGEWV AGTGEKLESH TPYSHELLWQ GYSASGEQVD AAVKAARRAF LDWKKRPFAE 70 80 90 100 110 120 REQKVLAFAE LVKANSEQIA QVIAKETGKP LWETRTEAAS IAGKIAISIR AYHERTGETV 130 140 150 160 170 180 REAAGNQLVL RHRPLGVMAV FGPYNFPGHL PNGHIVPALL AGNTVVFKPS EQTPWTGEVL 190 200 210 220 230 240 MQLWQQAGLP AGVINLVQGS KETGIALAQS RGIDGLLFTG SANTGHLLHR QFAGQPDKML 250 260 270 280 290 300 ALEMGGNNPM VISEHYGDLD ATVYTIIQSA FISSGQRCTC VRRLYVPQGT KGDALLDKLV 310 320 330 340 350 360 SVTAKLRIDQ PFAEPAPFMG PLVSEAAAQA ILKAQADLQA LGGKSLLEAR ALHAAFITPA 370 380 390 400 410 420 IIDVTAIERL PDDEYFGPLL QVVRYQTLAQ AVELANDTRF GLSAGLVSTD DGEWDYFVEH 430 440 450 460 470 480 IRAGIVNRNR QLTGASGDAP FGGPGASGNL RPSAFYAADY CAYPMASMEG DTTLLPATLS PGVEL

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE003852 Genomic DNA. Translation: AAF95757.1.
PIR	D82054.
RefSeq	NP_232244.1.
3D structure databases
HSSP	HSSP built from PDB template 1BXS based on UniProtKB P51977.
ModBase	Search...
Genome annotation databases
GeneID	2615633.
GenomeReviews	Gene locus VC_2616 in contig AE003852_GR.
KEGG	vch:VC2616.
TIGR	VC_2616.
Phylogenomic databases
HOGENOM	Q9KNW4.
OMA	Q9KNW4. TNIAELP.
Enzyme and pathway databases
BRENDA	1.2.1.71. 19019.
Family and domain databases
HAMAP	MF_01174. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03240. arg_catab_astD. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_VIBCH

Accession

Primary (citable) accession number: Q9KNW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)