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Protein

Penicillin-binding protein 1A

Gene

mrcA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (By similarity).By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei471 – 4711Acyl-ester intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Short name:
PBP-1a
Short name:
PBP1a
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.2.-)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.-.-)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:mrcA
Synonyms:ponA
Ordered Locus Names:VC_2635
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini28 – 825798PeriplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 825825Penicillin-binding protein 1APRO_0000083175Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243277.VC2635.

Structurei

3D structure databases

ProteinModelPortaliQ9KNU5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 216169TransglycosylaseAdd
BLAST
Regioni413 – 752340TranspeptidaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108JQC. Bacteria.
COG5009. LUCA.
KOiK05366.
OMAiIAQVWVT.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.710.10. 2 hits.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR012340. NA-bd_OB-fold.
IPR031376. PCB_OB.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF17092. PCB_OB. 1 hit.
PF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 3 hits.

Sequencei

Sequence statusi: Complete.

Q9KNU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFIKRLLVF SLICIILGVT TIFGFYFYVK SDLPDVATLR DVQLQTPMQV
60 70 80 90 100
FSQDGKLIAQ FGEKRRIPLK LEEMPKELIE AVIATEDSRY YEHYGFDPIG
110 120 130 140 150
ITRAAFAVLA SGSASQGAST ITQQLARNFF LSNEKKVMRK VKEIFIAIHI
160 170 180 190 200
EQLLSKQEIL ELYLNKIYLG YRSYGVGAAA QAYFGKEVKD LTLGEIALIA
210 220 230 240 250
GLPKAPSTMN PIYSVERATN RRNVVLQRML DEKYITKAEY DAARAEPVLP
260 270 280 290 300
KYHGAEIELN APYVAEIARA WMVERYGEEA AYTSGMNVYT TVDSKLQRAA
310 320 330 340 350
NQAAINNLLA YDERHGYRGA EKELWQVNQP AWSSTQLSEY LSNEPTYGDM
360 370 380 390 400
FPAAVLSVEE KSAQVWVKSY GVQTIAWEDM NWARRFINDD RQGPLPKSAN
410 420 430 440 450
EFLAAGQQIW VRPRTQDGAI TAWKLTQVPN ANTAFVAMNP ENGAVTALVG
460 470 480 490 500
GFNFVHNKFN RATQSVRQVG SSIKPFIYSA ALNKGLTLAT LINDAPINQW
510 520 530 540 550
DESQGTAWRP KNSPPTYTGP TRLRIGLAQS KNVMAVRVLR EVGLDETREY
560 570 580 590 600
LTRFGFKLDQ LPRSETIALG AGSLTPVQMA QGFSVFANNG YFVEPFYISR
610 620 630 640 650
VENPFGNIEF SAEPKVVCHR ECSSELDEFA EQDAASPYAP KVISEQNAFL
660 670 680 690 700
TREMLYSNIW GGGEWSSDTG WNGTGWRAQA LKRRDIGGKT GTTNDSKDAW
710 720 730 740 750
YNGYAPGIVG VAWVGFDDHS RNLGKTAPNR NIEDDVSGAE SGGKTALPAW
760 770 780 790 800
VEFMSLALQD VPVQQKAVPN NIARVRIDRD TGLLTNKLDS SSMFEYFEAG
810 820
TEPTEYVSEH VNESIYSTSS GEELF
Length:825
Mass (Da):91,987
Last modified:June 20, 2001 - v2
Checksum:i8A9553DA2650B9D2
GO

Sequence cautioni

The sequence AAF95776 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95776.1. Different initiation.
PIRiB82051.
RefSeqiNP_232263.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95776; AAF95776; VC_2635.
GeneIDi2615652.
KEGGivch:VC2635.
PATRICi20084294. VBIVibCho83274_2513.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95776.1. Different initiation.
PIRiB82051.
RefSeqiNP_232263.1. NC_002505.1.

3D structure databases

ProteinModelPortaliQ9KNU5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC2635.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Protocols and materials databases

DNASUi2615652.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF95776; AAF95776; VC_2635.
GeneIDi2615652.
KEGGivch:VC2635.
PATRICi20084294. VBIVibCho83274_2513.

Phylogenomic databases

eggNOGiENOG4108JQC. Bacteria.
COG5009. LUCA.
KOiK05366.
OMAiIAQVWVT.

Enzyme and pathway databases

UniPathwayiUPA00219.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.710.10. 2 hits.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR012340. NA-bd_OB-fold.
IPR031376. PCB_OB.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF17092. PCB_OB. 1 hit.
PF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPBPA_VIBCH
AccessioniPrimary (citable) accession number: Q9KNU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: September 7, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.