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Protein

Penicillin-binding protein 1A

Gene

mrcA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).By similarity

Catalytic activityi

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.By similarity
Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Proton donor; for transglycosylase activityBy similarity1
Active sitei471Acyl-ester intermediate; for transpeptidase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Glycosyltransferase, Hydrolase, Protease, Transferase
Biological processAntibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Short name:
PBP-1a
Short name:
PBP1a
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.1.129By similarity)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.16.4By similarity)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:mrcA
Synonyms:ponA
Ordered Locus Names:VC_2635
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini28 – 825PeriplasmicSequence analysisAdd BLAST798

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000831751 – 825Penicillin-binding protein 1AAdd BLAST825

Interactioni

Protein-protein interaction databases

STRINGi243277.VC2635.

Structurei

3D structure databases

ProteinModelPortaliQ9KNU5.
SMRiQ9KNU5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 216TransglycosylaseAdd BLAST169
Regioni413 – 752TranspeptidaseAdd BLAST340

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108JQC. Bacteria.
COG5009. LUCA.
KOiK05366.
OMAiLAQMAMI.

Family and domain databases

Gene3Di1.10.3810.10. 1 hit.
InterProiView protein in InterPro
IPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR031376. PCB_OB.
IPR001460. PCN-bd_Tpept.
PfamiView protein in Pfam
PF17092. PCB_OB. 1 hit.
PF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 3 hits.

Sequencei

Sequence statusi: Complete.

Q9KNU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFIKRLLVF SLICIILGVT TIFGFYFYVK SDLPDVATLR DVQLQTPMQV
60 70 80 90 100
FSQDGKLIAQ FGEKRRIPLK LEEMPKELIE AVIATEDSRY YEHYGFDPIG
110 120 130 140 150
ITRAAFAVLA SGSASQGAST ITQQLARNFF LSNEKKVMRK VKEIFIAIHI
160 170 180 190 200
EQLLSKQEIL ELYLNKIYLG YRSYGVGAAA QAYFGKEVKD LTLGEIALIA
210 220 230 240 250
GLPKAPSTMN PIYSVERATN RRNVVLQRML DEKYITKAEY DAARAEPVLP
260 270 280 290 300
KYHGAEIELN APYVAEIARA WMVERYGEEA AYTSGMNVYT TVDSKLQRAA
310 320 330 340 350
NQAAINNLLA YDERHGYRGA EKELWQVNQP AWSSTQLSEY LSNEPTYGDM
360 370 380 390 400
FPAAVLSVEE KSAQVWVKSY GVQTIAWEDM NWARRFINDD RQGPLPKSAN
410 420 430 440 450
EFLAAGQQIW VRPRTQDGAI TAWKLTQVPN ANTAFVAMNP ENGAVTALVG
460 470 480 490 500
GFNFVHNKFN RATQSVRQVG SSIKPFIYSA ALNKGLTLAT LINDAPINQW
510 520 530 540 550
DESQGTAWRP KNSPPTYTGP TRLRIGLAQS KNVMAVRVLR EVGLDETREY
560 570 580 590 600
LTRFGFKLDQ LPRSETIALG AGSLTPVQMA QGFSVFANNG YFVEPFYISR
610 620 630 640 650
VENPFGNIEF SAEPKVVCHR ECSSELDEFA EQDAASPYAP KVISEQNAFL
660 670 680 690 700
TREMLYSNIW GGGEWSSDTG WNGTGWRAQA LKRRDIGGKT GTTNDSKDAW
710 720 730 740 750
YNGYAPGIVG VAWVGFDDHS RNLGKTAPNR NIEDDVSGAE SGGKTALPAW
760 770 780 790 800
VEFMSLALQD VPVQQKAVPN NIARVRIDRD TGLLTNKLDS SSMFEYFEAG
810 820
TEPTEYVSEH VNESIYSTSS GEELF
Length:825
Mass (Da):91,987
Last modified:June 20, 2001 - v2
Checksum:i8A9553DA2650B9D2
GO

Sequence cautioni

The sequence AAF95776 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95776.1. Different initiation.
PIRiB82051.
RefSeqiNP_232263.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95776; AAF95776; VC_2635.
GeneIDi2615652.
KEGGivch:VC2635.
PATRICifig|243277.26.peg.2513.

Similar proteinsi

Entry informationi

Entry nameiPBPA_VIBCH
AccessioniPrimary (citable) accession number: Q9KNU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: August 30, 2017
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families