Reviewed, UniProtKB/Swiss-Prot Q9KNT5 (ARGE_VIBCH)

Names and origin

Protein names

Recommended name:
    Acetylornithine deacetylase
      Short name=Acetylornithinase
      Short name=AO
    EC=3.5.1.16
Alternative name(s):
    N-acetylornithinase
      Short name=NAO

Gene names

Name:	argE
Ordered Locus Names:	VC_2645

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	N(2)-acetyl-L-ornithine + H2O = acetate + L-ornithine. HAMAP MF_01108
Cofactor	Binds 2 zinc or cobalt ions per subunit By similarity. Glutathione By similarity.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine from N(2)-acetyl-L-ornithine (linear): step 1/1. HAMAP MF_01108
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the peptidase M20A family. ArgE subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	Cobalt Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetylornithine deacetylase activity Inferred from electronic annotation. Source: HAMAP cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: InterPro protein dimerization activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Acetylornithine deacetylase HAMAP MF_01108		PRO_0000185251
Sites
	Active site	78	1	By similarity
	Active site	140	1	Proton acceptor By similarity
	Metal binding	76	1	Cobalt or zinc 1 By similarity
	Metal binding	108	1	Cobalt or zinc 1 By similarity
	Metal binding	108	1	Cobalt or zinc 2 By similarity
	Metal binding	141	1	Cobalt or zinc 2 By similarity
	Metal binding	165	1	Cobalt or zinc 1 By similarity
	Metal binding	351	1	Cobalt or zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KNT5-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: F0CC5EC1892ECE8D	FASTA	378	41,964
	10 20 30 40 50 60 MPLPSFLEVY EGLISTSSIS STDARWDEGN EQVIAKLADW LSALGFSIQI EQVAPNKQNL 70 80 90 100 110 120 IAKLGSGEGG LLLAGHSDTV PFDEGRWNYN PHALTQVNNR FYGLGTADMK GFFAFIYEAV 130 140 150 160 170 180 KNVDWSKQTK PLYVLATCDE ETTMLGARHF TENAPFKPDY CIIGEPTSLV PIRAHKGHVA 190 200 210 220 230 240 NAIRVTGKSG HSSNPALGVN AIEIMHEVLF ALMQLRDRLI KEYHHPGFEI PTPTLNLGHI 250 260 270 280 290 300 HGGDSPNRIC GCCELHYDVR PLPGISLDGL DNLMHDALRE VQQKWPGRIE LVPLHDPIPG 310 320 330 340 350 360 YECAHDHPFI HGISEICEQE AQTVNYCTEA PFLQQVCPTL VLGPGSIDQA HQPDEFLAFE 370 FIDPTVRVLS RAMQKYCF

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE003852 Genomic DNA. Translation: AAF95786.1.
PIR	C82049.
RefSeq	NP_232273.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M20.974.
Genome annotation databases
GeneID	2615662.
GenomeReviews	Gene locus VC_2645 in contig AE003852_GR.
KEGG	vch:VC2645.
TIGR	VC_2645.
Phylogenomic databases
HOGENOM	Q9KNT5.
OMA	RCETWID.
Enzyme and pathway databases
BRENDA	3.5.1.16. 19019.
Family and domain databases
HAMAP	MF_01108. [Tree]
InterPro	IPR010169. AcOrn-deacetyl. IPR001261. ArgE/DapE_CS. IPR002933. Peptidase_M20. IPR011650. Peptidase_M20_dimer. [Graphical view]
Pfam	PF07687. M20_dimer. 1 hit. PF01546. Peptidase_M20. 1 hit. [Graphical view]
TIGRFAMs	TIGR01892. AcOrn-deacetyl. 1 hit.
PROSITE	PS00758. ARGE_DAPE_CPG2_1. 1 hit. PS00759. ARGE_DAPE_CPG2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGE_VIBCH

Accession

Primary (citable) accession number: Q9KNT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)