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Q9KNM2

- SPOT_VIBCH

UniProt

Q9KNM2 - SPOT_VIBCH

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Protein

Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Gene

spoT

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance.By similarity

Catalytic activityi

Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.

Cofactori

Manganese.By similarity

Pathwayi

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular response to starvation Source: TIGR
  2. guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Manganese

Enzyme and pathway databases

BioCyciVCHO:VC2710-MONOMER.
UniPathwayiUPA00908; UER00886.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC:3.1.7.2)
Alternative name(s):
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name:
(ppGpp)ase
Gene namesi
Name:spoT
Ordered Locus Names:VC_2710
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584: Chromosome 1

Pathology & Biotechi

Disruption phenotypei

Essential for growth, it cannot be disrupted. It can be disrupted in a relA deletion strain (relA phosphorylates GTP to ppGpp).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 705705Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolasePRO_0000386418Add
BLAST

Interactioni

Subunit structurei

Interacts with CgtA/Obg (AC Q9KUS8) in a yeast 2-hybrid assay.1 Publication

Protein-protein interaction databases

DIPiDIP-60892N.
STRINGi243277.VC2710.

Structurei

3D structure databases

ProteinModelPortaliQ9KNM2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 144100HDAdd
BLAST
Domaini631 – 70575ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated
Contains 1 ACT domain.PROSITE-ProRule annotation
Contains 1 HD domain.Curated

Phylogenomic databases

eggNOGiCOG0317.
KOiK01139.
OMAiAMHPMRS.
OrthoDBiEOG6SV551.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KNM2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYLFDSLKDV AQEYLTEPQI EALRQSYVVA RDAHEGQTRS SGEPYIIHPV
60 70 80 90 100
AVARILAEMR LDLETLQAAL LHDVIEDCDV TKEDLDAHFG SSVAELVDGV
110 120 130 140 150
SKLDKLKFRD RKEAQAENFR KMVLAMVQDI RVILIKLADR TPNMRTLGAL
160 170 180 190 200
RPDKKRRIAR ETLEIYAPLA HRLGIHNIKT ELEELGFEAL YPNRYRVLKE
210 220 230 240 250
VVKAARGNRK EMIQRIHSEI EGRLQEVGLP ARVVGREKNL FSIYNKMKTK
260 270 280 290 300
EQRFHTIMDI YAFRIVVDTA DTCYRVLGQV HSLYKPRPAR MKDYIAVPKA
310 320 330 340 350
NGYQSLHTSM VGPHGVPVEV QIRTEDMDQM ADKGVAAHWS YKANSERGGT
360 370 380 390 400
TAQIKAQRWM QSLLELQQSA GNSFEFIENV KSDLFPDEIY VFTPKGRIVE
410 420 430 440 450
LPMGATAVDF AYAVHTDIGN TCVGARVDRT PYPLSQSLKS GQTVEIISAP
460 470 480 490 500
GARPNAAWLN YVVTSRARTK IRQVLKTMRR EDSITLGRRL LNHALGEHSV
510 520 530 540 550
NEIAPENISK VLSDLKIASM DDLLAAIGLG ELMSIVIARR LLGNADELTE
560 570 580 590 600
PSKSGGNKNK LPIRGAEGIL LTFANCCHPI PDDHIIAHVS PGRGLVVHRE
610 620 630 640 650
TCPNVRGYQK EPDKYMAVEW TKDYDQEFIT ELKVDMHNRQ GALAELTNVI
660 670 680 690 700
SKTGSNIHGL STEERDGRLY TVTVLLTTKD RVHLAGIMRK IRTMPHALKV

RRRKN
Length:705
Mass (Da):79,496
Last modified:October 1, 2000 - v1
Checksum:i08F756E3A576B07F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE003852 Genomic DNA. Translation: AAF95850.1.
PIRiB82044.
RefSeqiNP_232337.1. NC_002505.1.
WP_010895463.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95850; AAF95850; VC_2710.
GeneIDi2615538.
KEGGivch:VC2710.
PATRICi20084438. VBIVibCho83274_2585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE003852 Genomic DNA. Translation: AAF95850.1 .
PIRi B82044.
RefSeqi NP_232337.1. NC_002505.1.
WP_010895463.1. NC_002505.1.

3D structure databases

ProteinModelPortali Q9KNM2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60892N.
STRINGi 243277.VC2710.

Protocols and materials databases

DNASUi 2615538.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAF95850 ; AAF95850 ; VC_2710 .
GeneIDi 2615538.
KEGGi vch:VC2710.
PATRICi 20084438. VBIVibCho83274_2585.

Phylogenomic databases

eggNOGi COG0317.
KOi K01139.
OMAi AMHPMRS.
OrthoDBi EOG6SV551.

Enzyme and pathway databases

UniPathwayi UPA00908 ; UER00886 .
BioCyci VCHO:VC2710-MONOMER.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view ]
Pfami PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view ]
SMARTi SM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view ]
SUPFAMi SSF81271. SSF81271. 1 hit.
TIGRFAMsi TIGR00691. spoT_relA. 1 hit.
PROSITEi PS51671. ACT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.
  2. "Regulation of the stringent response is the essential function of the conserved bacterial G protein CgtA in Vibrio cholerae."
    Raskin D.M., Judson N., Mekalanos J.J.
    Proc. Natl. Acad. Sci. U.S.A. 104:4636-4641(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, DISRUPTION PHENOTYPE.
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiSPOT_VIBCH
AccessioniPrimary (citable) accession number: Q9KNM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: October 1, 2000
Last modified: October 1, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3