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Q9KNM2

- SPOT_VIBCH

UniProt

Q9KNM2 - SPOT_VIBCH

Protein

Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Gene

spoT

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance.By similarity

    Catalytic activityi

    Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.

    Cofactori

    Manganese.By similarity

    Pathwayi

    GO - Molecular functioni

    1. amino acid binding Source: InterPro
    2. guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular response to starvation Source: TIGR
    2. guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Manganese

    Enzyme and pathway databases

    BioCyciVCHO:VC2710-MONOMER.
    UniPathwayiUPA00908; UER00886.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC:3.1.7.2)
    Alternative name(s):
    Penta-phosphate guanosine-3'-pyrophosphohydrolase
    Short name:
    (ppGpp)ase
    Gene namesi
    Name:spoT
    Ordered Locus Names:VC_2710
    OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
    Taxonomic identifieri243277 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000000584: Chromosome 1

    Pathology & Biotechi

    Disruption phenotypei

    Essential for growth, it cannot be disrupted. It can be disrupted in a relA deletion strain (relA phosphorylates GTP to ppGpp).1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 705705Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolasePRO_0000386418Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with CgtA/Obg (AC Q9KUS8) in a yeast 2-hybrid assay.1 Publication

    Protein-protein interaction databases

    DIPiDIP-60892N.
    STRINGi243277.VC2710.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9KNM2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 144100HDAdd
    BLAST
    Domaini631 – 70575ACTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RelA/SpoT family.Curated
    Contains 1 ACT domain.PROSITE-ProRule annotation
    Contains 1 HD domain.Curated

    Phylogenomic databases

    eggNOGiCOG0317.
    KOiK01139.
    OMAiAMHPMRS.
    OrthoDBiEOG6SV551.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR002912. ACT_dom.
    IPR012675. Beta-grasp_dom.
    IPR003607. HD/PDEase_dom.
    IPR004811. RelA/Spo_fam.
    IPR007685. RelA_SpoT.
    IPR004095. TGS.
    IPR012676. TGS-like.
    [Graphical view]
    PfamiPF04607. RelA_SpoT. 1 hit.
    PF02824. TGS. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    SM00954. RelA_SpoT. 1 hit.
    [Graphical view]
    SUPFAMiSSF81271. SSF81271. 1 hit.
    TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
    PROSITEiPS51671. ACT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9KNM2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYLFDSLKDV AQEYLTEPQI EALRQSYVVA RDAHEGQTRS SGEPYIIHPV    50
    AVARILAEMR LDLETLQAAL LHDVIEDCDV TKEDLDAHFG SSVAELVDGV 100
    SKLDKLKFRD RKEAQAENFR KMVLAMVQDI RVILIKLADR TPNMRTLGAL 150
    RPDKKRRIAR ETLEIYAPLA HRLGIHNIKT ELEELGFEAL YPNRYRVLKE 200
    VVKAARGNRK EMIQRIHSEI EGRLQEVGLP ARVVGREKNL FSIYNKMKTK 250
    EQRFHTIMDI YAFRIVVDTA DTCYRVLGQV HSLYKPRPAR MKDYIAVPKA 300
    NGYQSLHTSM VGPHGVPVEV QIRTEDMDQM ADKGVAAHWS YKANSERGGT 350
    TAQIKAQRWM QSLLELQQSA GNSFEFIENV KSDLFPDEIY VFTPKGRIVE 400
    LPMGATAVDF AYAVHTDIGN TCVGARVDRT PYPLSQSLKS GQTVEIISAP 450
    GARPNAAWLN YVVTSRARTK IRQVLKTMRR EDSITLGRRL LNHALGEHSV 500
    NEIAPENISK VLSDLKIASM DDLLAAIGLG ELMSIVIARR LLGNADELTE 550
    PSKSGGNKNK LPIRGAEGIL LTFANCCHPI PDDHIIAHVS PGRGLVVHRE 600
    TCPNVRGYQK EPDKYMAVEW TKDYDQEFIT ELKVDMHNRQ GALAELTNVI 650
    SKTGSNIHGL STEERDGRLY TVTVLLTTKD RVHLAGIMRK IRTMPHALKV 700
    RRRKN 705
    Length:705
    Mass (Da):79,496
    Last modified:October 1, 2000 - v1
    Checksum:i08F756E3A576B07F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE003852 Genomic DNA. Translation: AAF95850.1.
    PIRiB82044.
    RefSeqiNP_232337.1. NC_002505.1.
    WP_010895463.1. NC_002505.1.

    Genome annotation databases

    EnsemblBacteriaiAAF95850; AAF95850; VC_2710.
    GeneIDi2615538.
    KEGGivch:VC2710.
    PATRICi20084438. VBIVibCho83274_2585.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE003852 Genomic DNA. Translation: AAF95850.1 .
    PIRi B82044.
    RefSeqi NP_232337.1. NC_002505.1.
    WP_010895463.1. NC_002505.1.

    3D structure databases

    ProteinModelPortali Q9KNM2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60892N.
    STRINGi 243277.VC2710.

    Protocols and materials databases

    DNASUi 2615538.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF95850 ; AAF95850 ; VC_2710 .
    GeneIDi 2615538.
    KEGGi vch:VC2710.
    PATRICi 20084438. VBIVibCho83274_2585.

    Phylogenomic databases

    eggNOGi COG0317.
    KOi K01139.
    OMAi AMHPMRS.
    OrthoDBi EOG6SV551.

    Enzyme and pathway databases

    UniPathwayi UPA00908 ; UER00886 .
    BioCyci VCHO:VC2710-MONOMER.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR002912. ACT_dom.
    IPR012675. Beta-grasp_dom.
    IPR003607. HD/PDEase_dom.
    IPR004811. RelA/Spo_fam.
    IPR007685. RelA_SpoT.
    IPR004095. TGS.
    IPR012676. TGS-like.
    [Graphical view ]
    Pfami PF04607. RelA_SpoT. 1 hit.
    PF02824. TGS. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    SM00954. RelA_SpoT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81271. SSF81271. 1 hit.
    TIGRFAMsi TIGR00691. spoT_relA. 1 hit.
    PROSITEi PS51671. ACT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39315 / El Tor Inaba N16961.
    2. "Regulation of the stringent response is the essential function of the conserved bacterial G protein CgtA in Vibrio cholerae."
      Raskin D.M., Judson N., Mekalanos J.J.
      Proc. Natl. Acad. Sci. U.S.A. 104:4636-4641(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, DISRUPTION PHENOTYPE.
      Strain: ATCC 39315 / El Tor Inaba N16961.

    Entry informationi

    Entry nameiSPOT_VIBCH
    AccessioniPrimary (citable) accession number: Q9KNM2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 13, 2009
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3