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Q9KNK0

- PCKA_VIBCH

UniProt

Q9KNK0 - PCKA_VIBCH

Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 3 (30 May 2003)
      Previous versions | rss
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    Functioni

    Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

    Catalytic activityi

    ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei67 – 671SubstrateUniRule annotation
    Binding sitei208 – 2081SubstrateUniRule annotation
    Metal bindingi214 – 2141ManganeseUniRule annotation
    Binding sitei214 – 2141ATPUniRule annotation
    Binding sitei214 – 2141SubstrateUniRule annotation
    Metal bindingi233 – 2331Manganese; via tele nitrogenUniRule annotation
    Binding sitei233 – 2331ATPUniRule annotation
    Metal bindingi270 – 2701ManganeseUniRule annotation
    Binding sitei298 – 2981ATPUniRule annotation
    Binding sitei334 – 3341ATPUniRule annotation
    Binding sitei334 – 3341SubstrateUniRule annotation
    Binding sitei456 – 4561ATPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi249 – 2579ATPUniRule annotation
    Nucleotide bindingi450 – 4512ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW
    3. phosphoenolpyruvate carboxykinase (ATP) activity Source: TIGR

    GO - Biological processi

    1. gluconeogenesis Source: TIGR

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    ATP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciVCHO:VC2738-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
    Short name:
    PCKUniRule annotation
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckAUniRule annotation
    Ordered Locus Names:VC_2738
    OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
    Taxonomic identifieri243277 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000000584: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 542542Phosphoenolpyruvate carboxykinase [ATP]PRO_0000203854Add
    BLAST

    Proteomic databases

    PRIDEiQ9KNK0.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243277.VC2738.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9KNK0.
    SMRiQ9KNK0. Positions 13-541.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1866.
    KOiK01610.
    OMAiGADPEHY.
    OrthoDBiEOG6DG2RK.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00453. PEPCK_ATP.
    InterProiIPR001272. PEP_carboxykinase_ATP.
    IPR013035. PEP_carboxykinase_C.
    IPR008210. PEP_carboxykinase_N.
    IPR015994. PEPCK_ATP_CS.
    [Graphical view]
    PfamiPF01293. PEPCK_ATP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    TIGRFAMsiTIGR00224. pckA. 1 hit.
    PROSITEiPS00532. PEPCK_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9KNK0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVMEHTKAA QIDLAQYGIT GVTELVRNPS YEMLFAEETR SDLEGYERGV    50
    VTELGAVAVD TGIFTGRSPK DKFIVKDDTT RDTLWWTSDK AKNDNKPINQ 100
    EVWNDLKALV TKQLSGKRVF VLDGYCGANA DTRLSVRFIT EVAWQAHFVK 150
    NMFIRPSEEE LAHFKPDFVV MNGAKCTNAK WKEHGLNSEN FTVFNLTERM 200
    QLIGGTWYGG EMKKGMFAMM NYFLPLQGIA SMHCSANMGK AGDVAIFFGL 250
    SGTGKTTLST DPKRALIGDD EHGWDDDGVF NFEGGCYAKT IKLSKEAEPD 300
    IYNAIRRDAL LENVTVRSDG SIDFDDGSKT ENTRVSYPIY HIDNIVKPVS 350
    KGGHATKVIF LSADAFGVLP PVSKLTPEQT KYHFLSGFTA KLAGTERGIT 400
    EPTPTFSACF GAAFLTLHPT QYAEVLVKRM EAAGAEAYLV NTGWNGSGKR 450
    ISIKDTRGII DAILDGSIEK AETKHIPIFN LQVPTALPGV DPMILDPRDT 500
    YVDPLQWESK AKDLATRFIN NFDKYTDNAE GKALVAAGPK LD 542
    Length:542
    Mass (Da):59,844
    Last modified:May 30, 2003 - v3
    Checksum:i3162609A820D8D14
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE003852 Genomic DNA. Translation: AAF95877.1.
    PIRiB82039.
    RefSeqiNP_232364.1. NC_002505.1.

    Genome annotation databases

    EnsemblBacteriaiAAF95877; AAF95877; VC_2738.
    GeneIDi2614901.
    KEGGivch:VC2738.
    PATRICi20084494. VBIVibCho83274_2613.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE003852 Genomic DNA. Translation: AAF95877.1 .
    PIRi B82039.
    RefSeqi NP_232364.1. NC_002505.1.

    3D structure databases

    ProteinModelPortali Q9KNK0.
    SMRi Q9KNK0. Positions 13-541.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243277.VC2738.

    Proteomic databases

    PRIDEi Q9KNK0.

    Protocols and materials databases

    DNASUi 2614901.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF95877 ; AAF95877 ; VC_2738 .
    GeneIDi 2614901.
    KEGGi vch:VC2738.
    PATRICi 20084494. VBIVibCho83274_2613.

    Phylogenomic databases

    eggNOGi COG1866.
    KOi K01610.
    OMAi GADPEHY.
    OrthoDBi EOG6DG2RK.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci VCHO:VC2738-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00453. PEPCK_ATP.
    InterProi IPR001272. PEP_carboxykinase_ATP.
    IPR013035. PEP_carboxykinase_C.
    IPR008210. PEP_carboxykinase_N.
    IPR015994. PEPCK_ATP_CS.
    [Graphical view ]
    Pfami PF01293. PEPCK_ATP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006294. PEP_crbxkin. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    TIGRFAMsi TIGR00224. pckA. 1 hit.
    PROSITEi PS00532. PEPCK_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39315 / El Tor Inaba N16961.

    Entry informationi

    Entry nameiPCKA_VIBCH
    AccessioniPrimary (citable) accession number: Q9KNK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: May 30, 2003
    Last modified: October 1, 2014
    This is version 87 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3