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Q9KNK0

- PCKA_VIBCH

UniProt

Q9KNK0 - PCKA_VIBCH

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Protein
Phosphoenolpyruvate carboxykinase [ATP]
Gene
pckA, VC_2738
Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA By similarity.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei67 – 671Substrate By similarity
Binding sitei208 – 2081Substrate By similarity
Metal bindingi214 – 2141Manganese By similarity
Binding sitei214 – 2141ATP By similarity
Binding sitei214 – 2141Substrate By similarity
Metal bindingi233 – 2331Manganese; via tele nitrogen By similarity
Binding sitei233 – 2331ATP By similarity
Metal bindingi270 – 2701Manganese By similarity
Binding sitei298 – 2981ATP By similarity
Binding sitei334 – 3341ATP By similarity
Binding sitei334 – 3341Substrate By similarity
Binding sitei456 – 4561ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi249 – 2579ATP By similarity
Nucleotide bindingi450 – 4512ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (ATP) activity Source: TIGR
Complete GO annotation...

GO - Biological processi

  1. gluconeogenesis Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciVCHO:VC2738-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP] (EC:4.1.1.49)
Short name:
PCK
Short name:
PEP carboxykinase
Short name:
PEPCK
Gene namesi
Name:pckA
Ordered Locus Names:VC_2738
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 542542Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation
PRO_0000203854Add
BLAST

Proteomic databases

PRIDEiQ9KNK0.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi243277.VC2738.

Structurei

3D structure databases

ProteinModelPortaliQ9KNK0.
SMRiQ9KNK0. Positions 13-541.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1866.
KOiK01610.
OMAiGADPEHY.
OrthoDBiEOG6DG2RK.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KNK0-1 [UniParc]FASTAAdd to Basket

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MTVMEHTKAA QIDLAQYGIT GVTELVRNPS YEMLFAEETR SDLEGYERGV    50
VTELGAVAVD TGIFTGRSPK DKFIVKDDTT RDTLWWTSDK AKNDNKPINQ 100
EVWNDLKALV TKQLSGKRVF VLDGYCGANA DTRLSVRFIT EVAWQAHFVK 150
NMFIRPSEEE LAHFKPDFVV MNGAKCTNAK WKEHGLNSEN FTVFNLTERM 200
QLIGGTWYGG EMKKGMFAMM NYFLPLQGIA SMHCSANMGK AGDVAIFFGL 250
SGTGKTTLST DPKRALIGDD EHGWDDDGVF NFEGGCYAKT IKLSKEAEPD 300
IYNAIRRDAL LENVTVRSDG SIDFDDGSKT ENTRVSYPIY HIDNIVKPVS 350
KGGHATKVIF LSADAFGVLP PVSKLTPEQT KYHFLSGFTA KLAGTERGIT 400
EPTPTFSACF GAAFLTLHPT QYAEVLVKRM EAAGAEAYLV NTGWNGSGKR 450
ISIKDTRGII DAILDGSIEK AETKHIPIFN LQVPTALPGV DPMILDPRDT 500
YVDPLQWESK AKDLATRFIN NFDKYTDNAE GKALVAAGPK LD 542
Length:542
Mass (Da):59,844
Last modified:May 30, 2003 - v3
Checksum:i3162609A820D8D14
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE003852 Genomic DNA. Translation: AAF95877.1.
PIRiB82039.
RefSeqiNP_232364.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95877; AAF95877; VC_2738.
GeneIDi2614901.
KEGGivch:VC2738.
PATRICi20084494. VBIVibCho83274_2613.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE003852 Genomic DNA. Translation: AAF95877.1 .
PIRi B82039.
RefSeqi NP_232364.1. NC_002505.1.

3D structure databases

ProteinModelPortali Q9KNK0.
SMRi Q9KNK0. Positions 13-541.
ModBasei Search...

Protein-protein interaction databases

STRINGi 243277.VC2738.

Proteomic databases

PRIDEi Q9KNK0.

Protocols and materials databases

DNASUi 2614901.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAF95877 ; AAF95877 ; VC_2738 .
GeneIDi 2614901.
KEGGi vch:VC2738.
PATRICi 20084494. VBIVibCho83274_2613.

Phylogenomic databases

eggNOGi COG1866.
KOi K01610.
OMAi GADPEHY.
OrthoDBi EOG6DG2RK.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci VCHO:VC2738-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00453. PEPCK_ATP.
InterProi IPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view ]
Pfami PF01293. PEPCK_ATP. 1 hit.
[Graphical view ]
PIRSFi PIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
TIGRFAMsi TIGR00224. pckA. 1 hit.
PROSITEi PS00532. PEPCK_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiPCKA_VIBCH
AccessioniPrimary (citable) accession number: Q9KNK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 30, 2003
Last modified: May 14, 2014
This is version 86 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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