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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei67 – 671SubstrateUniRule annotation
Binding sitei208 – 2081SubstrateUniRule annotation
Metal bindingi214 – 2141ManganeseUniRule annotation
Binding sitei214 – 2141ATPUniRule annotation
Binding sitei214 – 2141SubstrateUniRule annotation
Metal bindingi233 – 2331Manganese; via tele nitrogenUniRule annotation
Binding sitei233 – 2331ATPUniRule annotation
Metal bindingi270 – 2701ManganeseUniRule annotation
Binding sitei298 – 2981ATPUniRule annotation
Binding sitei334 – 3341ATPUniRule annotation
Binding sitei334 – 3341SubstrateUniRule annotation
Binding sitei456 – 4561ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi249 – 2579ATPUniRule annotation
Nucleotide bindingi450 – 4512ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (ATP) activity Source: TIGR

GO - Biological processi

  1. gluconeogenesis Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciVCHO:VC2738-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:VC_2738
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 542542Phosphoenolpyruvate carboxykinase [ATP]PRO_0000203854Add
BLAST

Proteomic databases

PRIDEiQ9KNK0.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243277.VC2738.

Structurei

3D structure databases

ProteinModelPortaliQ9KNK0.
SMRiQ9KNK0. Positions 13-541.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1866.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiEOG6DG2RK.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KNK0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTVMEHTKAA QIDLAQYGIT GVTELVRNPS YEMLFAEETR SDLEGYERGV
60 70 80 90 100
VTELGAVAVD TGIFTGRSPK DKFIVKDDTT RDTLWWTSDK AKNDNKPINQ
110 120 130 140 150
EVWNDLKALV TKQLSGKRVF VLDGYCGANA DTRLSVRFIT EVAWQAHFVK
160 170 180 190 200
NMFIRPSEEE LAHFKPDFVV MNGAKCTNAK WKEHGLNSEN FTVFNLTERM
210 220 230 240 250
QLIGGTWYGG EMKKGMFAMM NYFLPLQGIA SMHCSANMGK AGDVAIFFGL
260 270 280 290 300
SGTGKTTLST DPKRALIGDD EHGWDDDGVF NFEGGCYAKT IKLSKEAEPD
310 320 330 340 350
IYNAIRRDAL LENVTVRSDG SIDFDDGSKT ENTRVSYPIY HIDNIVKPVS
360 370 380 390 400
KGGHATKVIF LSADAFGVLP PVSKLTPEQT KYHFLSGFTA KLAGTERGIT
410 420 430 440 450
EPTPTFSACF GAAFLTLHPT QYAEVLVKRM EAAGAEAYLV NTGWNGSGKR
460 470 480 490 500
ISIKDTRGII DAILDGSIEK AETKHIPIFN LQVPTALPGV DPMILDPRDT
510 520 530 540
YVDPLQWESK AKDLATRFIN NFDKYTDNAE GKALVAAGPK LD
Length:542
Mass (Da):59,844
Last modified:May 30, 2003 - v3
Checksum:i3162609A820D8D14
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95877.1.
PIRiB82039.
RefSeqiNP_232364.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95877; AAF95877; VC_2738.
GeneIDi2614901.
KEGGivch:VC2738.
PATRICi20084494. VBIVibCho83274_2613.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95877.1.
PIRiB82039.
RefSeqiNP_232364.1. NC_002505.1.

3D structure databases

ProteinModelPortaliQ9KNK0.
SMRiQ9KNK0. Positions 13-541.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC2738.

Proteomic databases

PRIDEiQ9KNK0.

Protocols and materials databases

DNASUi2614901.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF95877; AAF95877; VC_2738.
GeneIDi2614901.
KEGGivch:VC2738.
PATRICi20084494. VBIVibCho83274_2613.

Phylogenomic databases

eggNOGiCOG1866.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiEOG6DG2RK.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciVCHO:VC2738-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiPCKA_VIBCH
AccessioniPrimary (citable) accession number: Q9KNK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 30, 2003
Last modified: January 7, 2015
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.