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Protein

Glutamine synthetase

Gene

glnAv

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi130Magnesium 1By similarity1
Metal bindingi132Magnesium 2By similarity1
Binding sitei208ATPBy similarity1
Metal bindingi213Magnesium 2By similarity1
Metal bindingi221Magnesium 2By similarity1
Binding sitei266L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi270Magnesium 1; via pros nitrogenBy similarity1
Binding sitei274ATPBy similarity1
Binding sitei322L-glutamateBy similarity1
Binding sitei328L-glutamateBy similarity1
Binding sitei340ATPBy similarity1
Binding sitei340L-glutamateBy similarity1
Binding sitei345ATPBy similarity1
Binding sitei353ATPBy similarity1
Metal bindingi358Magnesium 1By similarity1
Binding sitei360L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi272 – 274ATPBy similarity3

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamate-ammonia ligase activity Source: TIGR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • glutamine biosynthetic process Source: TIGR
  • nitrogen fixation Source: InterPro

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciVCHO:VC2746-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseCurated
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnAv
Ordered Locus Names:VC_2746
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532761 – 469Glutamine synthetaseAdd BLAST469

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei398O-AMP-tyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9KNJ2.

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexameric ring.By similarity

Protein-protein interaction databases

STRINGi243277.VC2746.

Structurei

3D structure databases

ProteinModelPortaliQ9KNJ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 266L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
KOiK01915.
OMAiKVLNQVG.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KNJ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVENVLSLI QENEVKFVDL RFTDTKGKEQ HISIPAHQID ADFFEDGKMF
60 70 80 90 100
DGSSVAGWKG INESDMVMMP DPSSAVLDPF TEDATLNIRC DILEPATMQG
110 120 130 140 150
YDRDPRSIAK RAEEYMRSTG IADTVLVGPE PEFFLFDDVK FATNMSGSFF
160 170 180 190 200
KIDDVEAAWN TGTEYEDGNK GHRPGVKGGY FPVAPVDSSQ DIRSAMCLIM
210 220 230 240 250
EEMGLVVEAH HHEVATAGQN EIATRFNTLT TKADEIQIYK YVVHNVAHAF
260 270 280 290 300
GKTATFMPKP LVGDNGSGMH VHQSLAKDGV NLFAGDKYGG LSETALYYIG
310 320 330 340 350
GIIKHARALN AITNPSTNSY KRLVPHYEAP VMLAYSARNR SASIRIPVVP
360 370 380 390 400
SPKARRIEVR FPDPAANPYL AFAAMLMAGL DGIKNKIHPG EAMDKDLYDL
410 420 430 440 450
PAEEAAEIPK VAESLQQALQ YLDADREFLT AGGVFSDDFI DSYIALKTKD
460
VERVNVAVHP LEFELYYSV
Length:469
Mass (Da):51,751
Last modified:October 1, 2000 - v1
Checksum:i0B8E33BFB182EEA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95885.1.
PIRiA82038.
RefSeqiNP_232372.1. NC_002505.1.
WP_000110151.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95885; AAF95885; VC_2746.
GeneIDi2614909.
KEGGivch:VC2746.
PATRICi20084512. VBIVibCho83274_2622.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF95885.1.
PIRiA82038.
RefSeqiNP_232372.1. NC_002505.1.
WP_000110151.1. NC_002505.1.

3D structure databases

ProteinModelPortaliQ9KNJ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC2746.

Proteomic databases

PRIDEiQ9KNJ2.

Protocols and materials databases

DNASUi2614909.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF95885; AAF95885; VC_2746.
GeneIDi2614909.
KEGGivch:VC2746.
PATRICi20084512. VBIVibCho83274_2622.

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
KOiK01915.
OMAiKVLNQVG.

Enzyme and pathway databases

BioCyciVCHO:VC2746-MONOMER.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLN1B_VIBCH
AccessioniPrimary (citable) accession number: Q9KNJ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 1, 2000
Last modified: April 12, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.