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Q9KNE8 (GLGB_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:VC_A0016
OrganismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]
Taxonomic identifier243277 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length666 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6666661,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188759

Sites

Active site3451Nucleophile By similarity
Active site3981Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KNE8 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: BA538EBD93DE9868

FASTA66677,096
        10         20         30         40         50         60 
MKAEGGFVLK DGRNLRFTHY QLAVDWAGTE QLLDDPYQYH GLYAEYEDLH TPKQMYHHMG 

        70         80         90        100        110        120 
AQFVTLERDG KMVSGVRFLV YAPHAAACSL IGAFNHWDGR RHPMQRLDYG IWGIFIPGLP 

       130        140        150        160        170        180 
EGTQYKFELK GPHGEGLPHK ADPWGFYAEQ YPSFASVTYD HRRYQWQDTA WQQRPVTEKR 

       190        200        210        220        230        240 
KQALSFYELH VGSWKRGENG EFLNYRELAD QLVPYLVEMG YTHVELMPVA EHPFYGSWGY 

       250        260        270        280        290        300 
QPVGLFAPTS RYGSPDDFKY FVDLCHQAGI GVVLDWVPAH FPSDSHGLAN FDGTPLFHDP 

       310        320        330        340        350        360 
DPRRGWHQDW NSYIYDLGRE HVRRFLVANA LYWFEMFHID GIRVDAVASM LYLDYSRSHD 

       370        380        390        400        410        420 
QWIPNVDGGR ENYDAIATFK WMNEEVYKHF PNAMTIAEES TAFPGVSAPT FMGGLGFGFK 

       430        440        450        460        470        480 
WNMGWMHDSL SYIKEDPVHR KYHHNTLTFP LIYAFSENYV LSLSHDEVVY GKRSLMYKMP 

       490        500        510        520        530        540 
GDEWQQTANL RAYLGYMYGQ PGKKLNFMGT ELGQTAEWDH DGQLQWFLTQ FERHAGIQRL 

       550        560        570        580        590        600 
VRDLNHLYQA QTALHQLDCD PRGFEWRLQD NADLSVIAHE RMDEAGNRVL VITNFTPVPQ 

       610        620        630        640        650        660 
QEFRLGVPKT GKYRLLLNTD AKQYNGSDYP VLQDVSTEAI SSEGLDQSLL LSVPPLATLF 


YQWSAK 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE003853 Genomic DNA. Translation: AAF95930.1.
PIRD82511.

3D structure databases

ProteinModelPortalQ9KNE8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243277.VCA0016.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

DNASU2612506.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF95930; AAF95930; VC_A0016.
PATRIC20084623. VBIVibCho83274_2663.

Phylogenomic databases

eggNOGCOG0296.
OMAGELSHAW.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycVCHO:VCA0016-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_VIBCH
AccessionPrimary (citable) accession number: Q9KNE8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries