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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei408NucleophileUniRule annotation1
Active sitei461Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • glycogen biosynthetic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciVCHO:VCA0015-MONOMER.
VCHO:VCA0016-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:VC_A0015/VC_A0016
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001887591 – 7291,4-alpha-glucan branching enzyme GlgBAdd BLAST729

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243277.VCA0016.

Structurei

3D structure databases

ProteinModelPortaliQ9KNE8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9C. Bacteria.
COG0296. LUCA.
OMAiCHQVGIG.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KNE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKITKKPSKV QQFYDQLARA AFADPFSFLG PYIPAEQGAL RVWMPGADNV
60 70 80 90 100
ALVVEGQARV ALEREGEGGF VLKDGRNLRF THYQLAVDWA GTEQLLDDPY
110 120 130 140 150
QYHGLYAEYE DLHTPKQMYH HMGAQFVTLE RDGKMVSGVR FLVYAPHAAA
160 170 180 190 200
CSLIGAFNHW DGRRHPMQRL DYGIWGIFIP GLPEGTQYKF ELKGPHGEGL
210 220 230 240 250
PHKADPWGFY AEQYPSFASV TYDHRRYQWQ DTAWQQRPVT EKRKQALSFY
260 270 280 290 300
ELHVGSWKRG ENGEFLNYRE LADQLVPYLV EMGYTHVELM PVAEHPFYGS
310 320 330 340 350
WGYQPVGLFA PTSRYGSPDD FKYFVDLCHQ AGIGVVLDWV PAHFPSDSHG
360 370 380 390 400
LANFDGTPLF HDPDPRRGWH QDWNSYIYDL GREHVRRFLV ANALYWFEMF
410 420 430 440 450
HIDGIRVDAV ASMLYLDYSR SHDQWIPNVD GGRENYDAIA TFKWMNEEVY
460 470 480 490 500
KHFPNAMTIA EESTAFPGVS APTFMGGLGF GFKWNMGWMH DSLSYIKEDP
510 520 530 540 550
VHRKYHHNTL TFPLIYAFSE NYVLSLSHDE VVYGKRSLMY KMPGDEWQQT
560 570 580 590 600
ANLRAYLGYM YGQPGKKLNF MGTELGQTAE WDHDGQLQWF LTQFERHAGI
610 620 630 640 650
QRLVRDLNHL YQAQTALHQL DCDPRGFEWR LQDNADLSVI AHERMDEAGN
660 670 680 690 700
RVLVITNFTP VPQQEFRLGV PKTGKYRLLL NTDAKQYNGS DYPVLQDVST
710 720
EAISSEGLDQ SLLLSVPPLA TLFYQWSAK
Length:729
Mass (Da):84,027
Last modified:October 5, 2016 - v2
Checksum:iECDE22002582E543
GO

Sequence cautioni

The sequence AAF95929 differs from that shown. Reason: Frameshift at position 66.Curated
The sequence AAF95930 differs from that shown. Reason: Frameshift at position 66.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA. Translation: AAF95929.1. Frameshift.
AE003853 Genomic DNA. Translation: AAF95930.1. Frameshift.
PIRiD82511.

Genome annotation databases

EnsemblBacteriaiAAF95929; AAF95929; VC_A0015.
AAF95930; AAF95930; VC_A0016.
PATRICi20084623. VBIVibCho83274_2663.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA. Translation: AAF95929.1. Frameshift.
AE003853 Genomic DNA. Translation: AAF95930.1. Frameshift.
PIRiD82511.

3D structure databases

ProteinModelPortaliQ9KNE8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VCA0016.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

DNASUi2612506.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF95929; AAF95929; VC_A0015.
AAF95930; AAF95930; VC_A0016.
PATRICi20084623. VBIVibCho83274_2663.

Phylogenomic databases

eggNOGiENOG4105C9C. Bacteria.
COG0296. LUCA.
OMAiCHQVGIG.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciVCHO:VCA0015-MONOMER.
VCHO:VCA0016-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLGB_VIBCH
AccessioniPrimary (citable) accession number: Q9KNE8
Secondary accession number(s): Q9KNE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: October 5, 2016
Last modified: November 30, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.