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Protein

Nicotinate phosphoribosyltransferase

Gene

pncB

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.UniRule annotation

Catalytic activityi

Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate D-ribonucleotide from nicotinate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinate phosphoribosyltransferase (pncB)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate D-ribonucleotide from nicotinate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • NAD biosynthetic process Source: UniProtKB-HAMAP
  • NADH metabolic process Source: TIGR
  • nicotinate nucleotide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Enzyme and pathway databases

BioCyciVCHO:VCA0098-MONOMER.
UniPathwayiUPA00253; UER00457.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate phosphoribosyltransferaseUniRule annotation (EC:6.3.4.21UniRule annotation)
Short name:
NAPRTaseUniRule annotation
Gene namesi
Name:pncBUniRule annotation
Ordered Locus Names:VC_A0098
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002058471 – 435Nicotinate phosphoribosyltransferaseAdd BLAST435

Interactioni

Protein-protein interaction databases

STRINGi243277.VCA0098.

Structurei

Secondary structure

1435
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni3 – 5Combined sources3
Beta strandi16 – 18Combined sources3
Helixi19 – 31Combined sources13
Beta strandi36 – 45Combined sources10
Turni47 – 49Combined sources3
Helixi50 – 62Combined sources13
Helixi63 – 65Combined sources3
Helixi70 – 79Combined sources10
Helixi85 – 91Combined sources7
Helixi98 – 101Combined sources4
Beta strandi102 – 107Combined sources6
Beta strandi114 – 122Combined sources9
Helixi123 – 126Combined sources4
Helixi129 – 145Combined sources17
Helixi153 – 172Combined sources20
Beta strandi179 – 181Combined sources3
Helixi190 – 203Combined sources14
Helixi205 – 207Combined sources3
Beta strandi208 – 213Combined sources6
Helixi214 – 220Combined sources7
Helixi230 – 239Combined sources10
Helixi242 – 244Combined sources3
Helixi245 – 256Combined sources12
Beta strandi263 – 266Combined sources4
Helixi271 – 277Combined sources7
Helixi280 – 285Combined sources6
Beta strandi288 – 291Combined sources4
Helixi296 – 309Combined sources14
Helixi314 – 316Combined sources3
Beta strandi317 – 321Combined sources5
Helixi327 – 337Combined sources11
Turni338 – 340Combined sources3
Beta strandi341 – 347Combined sources7
Helixi349 – 352Combined sources4
Beta strandi370 – 378Combined sources9
Helixi390 – 392Combined sources3
Helixi398 – 407Combined sources10
Helixi414 – 425Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HL7X-ray1.80A/B2-435[»]
ProteinModelPortaliQ9KN67.
SMRiQ9KN67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAPRTase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107TX3. Bacteria.
COG1488. LUCA.
KOiK00763.
OMAiKLTMQCA.

Family and domain databases

HAMAPiMF_00570. NAPRTase. 1 hit.
InterProiIPR006406. Nic_PRibTrfase.
IPR007229. Nic_PRibTrfase-Fam.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view]
PANTHERiPTHR11098. PTHR11098. 1 hit.
PfamiPF04095. NAPRTase. 1 hit.
[Graphical view]
PIRSFiPIRSF000484. NAPRT. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR01514. NAPRTase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KN67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPRLFSPHI IRSLLDLDAY KINMMQAIHH FYPDVSVRYE LIVRSEEDAS
60 70 80 90 100
GLLDAIRQEI AHLGTLRFSD ADIHYLTQHA PHLKATFLQS LRYFHFVPQE
110 120 130 140 150
QVEMGIVKQG GKQQLRISIR GSWRDTILYE TLVMAIVSEV RSRQRWAEVP
160 170 180 190 200
ADLPLKVLKT KLDQLKAEIE RRGINNFSLT EMGTRRRFSS QVQRDVLACL
210 220 230 240 250
KQEIPQWVLG TSNYHFAREF DLKPIGTIAH EWFMGHQALV NERDSQQVAL
260 270 280 290 300
ERWLTAFDGM LAIAPTDTLT IDAFLNDFNR HLANAYDGVR HDSGCPFRWG
310 320 330 340 350
DKMIAHYQQL GIDPTTKLFI FSDGLDFDQA LELCEYFAGR VKISFGIGTF
360 370 380 390 400
LTNDLANWRN AAGVEYRPLS IVIKLAECQG RPVAKISDQP EKAMCEDPIF
410 420 430
LANLKRRFNI ELDVDALIQE LRHQKRSPRH YISAA
Length:435
Mass (Da):50,078
Last modified:October 1, 2000 - v1
Checksum:i2CEF5D950C6C11FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA. Translation: AAF96012.1.
PIRiH82501.
RefSeqiNP_232499.1. NC_002506.1.
WP_001069589.1. NC_002506.1.

Genome annotation databases

EnsemblBacteriaiAAF96012; AAF96012; VC_A0098.
GeneIDi2612706.
KEGGivch:VCA0098.
PATRICi20084775. VBIVibCho83274_2739.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA. Translation: AAF96012.1.
PIRiH82501.
RefSeqiNP_232499.1. NC_002506.1.
WP_001069589.1. NC_002506.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HL7X-ray1.80A/B2-435[»]
ProteinModelPortaliQ9KN67.
SMRiQ9KN67.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VCA0098.

Protocols and materials databases

DNASUi2612706.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF96012; AAF96012; VC_A0098.
GeneIDi2612706.
KEGGivch:VCA0098.
PATRICi20084775. VBIVibCho83274_2739.

Phylogenomic databases

eggNOGiENOG4107TX3. Bacteria.
COG1488. LUCA.
KOiK00763.
OMAiKLTMQCA.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00457.
BioCyciVCHO:VCA0098-MONOMER.

Family and domain databases

HAMAPiMF_00570. NAPRTase. 1 hit.
InterProiIPR006406. Nic_PRibTrfase.
IPR007229. Nic_PRibTrfase-Fam.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view]
PANTHERiPTHR11098. PTHR11098. 1 hit.
PfamiPF04095. NAPRTase. 1 hit.
[Graphical view]
PIRSFiPIRSF000484. NAPRT. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR01514. NAPRTase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPNCB_VIBCH
AccessioniPrimary (citable) accession number: Q9KN67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.