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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 508ATPUniRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • NAD+ synthase (glutamine-hydrolyzing) activity Source: TIGR
  • NAD+ synthase activity Source: GO_Central

GO - Biological processi

  • NAD biosynthetic process Source: GO_Central
  • NADH metabolic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciVCHO:VCA0207-MONOMER.
UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:VC_A0207
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276NH(3)-dependent NAD(+) synthetasePRO_0000152215Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243277.VCA0207.

Structurei

Secondary structure

276
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 99Combined sources
Helixi17 – 3519Combined sources
Beta strandi39 – 435Combined sources
Helixi48 – 6720Combined sources
Beta strandi74 – 796Combined sources
Beta strandi82 – 843Combined sources
Helixi88 – 9811Combined sources
Beta strandi101 – 1055Combined sources
Helixi109 – 12618Combined sources
Helixi136 – 15924Combined sources
Beta strandi161 – 1644Combined sources
Helixi169 – 1746Combined sources
Turni179 – 1835Combined sources
Turni189 – 1924Combined sources
Helixi195 – 20410Combined sources
Helixi209 – 2124Combined sources
Helixi237 – 2459Combined sources
Helixi251 – 26313Combined sources
Helixi265 – 2684Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3Q4GX-ray2.40A/B1-276[»]
ProteinModelPortaliQ9KMW1.
SMRiQ9KMW1. Positions 1-276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RA1. Bacteria.
COG0171. LUCA.
KOiK01916.
OMAiDFVRGNI.
OrthoDBiEOG64JFM7.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KMW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHKIREEMR VLPSIDPQFE IERRVAFIKR KLTEARCKSL VLGISGGVDS
60 70 80 90 100
TTCGRLAQLA VEELNQQHNT TEYQFIAVRL PYGEQKDEDE AQLALSFIRP
110 120 130 140 150
THSVSVNIKA GVDGLHAASH HALANTGLIP SDPAKVDFIK GNVKARARMV
160 170 180 190 200
AQYEIAGYVG GLVLGTDHSA ENITGFYTKF GDGACDLAPL FGLNKRQVRL
210 220 230 240 250
LAKTLGAPEQ LVYKTPTADL EELAPQKADE AALNLTYEQI DDFLEGKAVP
260 270
AEVSQRLVAI YHATQHKRQP IPTIYD
Length:276
Mass (Da):30,403
Last modified:October 1, 2000 - v1
Checksum:i94F016DE81052A89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA. Translation: AAF96119.1.
PIRiH82488.
RefSeqiNP_232606.1. NC_002506.1.
WP_000400328.1. NC_002506.1.

Genome annotation databases

EnsemblBacteriaiAAF96119; AAF96119; VC_A0207.
GeneIDi2612343.
KEGGivch:VCA0207.
PATRICi20084981. VBIVibCho83274_2842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA. Translation: AAF96119.1.
PIRiH82488.
RefSeqiNP_232606.1. NC_002506.1.
WP_000400328.1. NC_002506.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3Q4GX-ray2.40A/B1-276[»]
ProteinModelPortaliQ9KMW1.
SMRiQ9KMW1. Positions 1-276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VCA0207.

Protocols and materials databases

DNASUi2612343.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF96119; AAF96119; VC_A0207.
GeneIDi2612343.
KEGGivch:VCA0207.
PATRICi20084981. VBIVibCho83274_2842.

Phylogenomic databases

eggNOGiENOG4107RA1. Bacteria.
COG0171. LUCA.
KOiK01916.
OMAiDFVRGNI.
OrthoDBiEOG64JFM7.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.
BioCyciVCHO:VCA0207-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiNADE_VIBCH
AccessioniPrimary (citable) accession number: Q9KMW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2000
Last modified: February 17, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.