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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei45UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi43 – 50ATPUniRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • NAD+ synthase (glutamine-hydrolyzing) activity Source: TIGR
  • NAD+ synthase activity Source: GO_Central

GO - Biological processi

  • NAD biosynthetic process Source: GO_Central
  • NADH metabolic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciVCHO:VCA0207-MONOMER.
UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:VC_A0207
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001522151 – 276NH(3)-dependent NAD(+) synthetaseAdd BLAST276

Interactioni

Protein-protein interaction databases

STRINGi243277.VCA0207.

Structurei

Secondary structure

1276
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 9Combined sources9
Helixi17 – 35Combined sources19
Beta strandi39 – 43Combined sources5
Helixi48 – 67Combined sources20
Beta strandi74 – 79Combined sources6
Beta strandi82 – 84Combined sources3
Helixi88 – 98Combined sources11
Beta strandi101 – 105Combined sources5
Helixi109 – 126Combined sources18
Helixi136 – 159Combined sources24
Beta strandi161 – 164Combined sources4
Helixi169 – 174Combined sources6
Turni179 – 183Combined sources5
Turni189 – 192Combined sources4
Helixi195 – 204Combined sources10
Helixi209 – 212Combined sources4
Helixi237 – 245Combined sources9
Helixi251 – 263Combined sources13
Helixi265 – 268Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3Q4GX-ray2.40A/B1-276[»]
ProteinModelPortaliQ9KMW1.
SMRiQ9KMW1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RA1. Bacteria.
COG0171. LUCA.
KOiK01916.
OMAiAKIIEGW.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KMW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHKIREEMR VLPSIDPQFE IERRVAFIKR KLTEARCKSL VLGISGGVDS
60 70 80 90 100
TTCGRLAQLA VEELNQQHNT TEYQFIAVRL PYGEQKDEDE AQLALSFIRP
110 120 130 140 150
THSVSVNIKA GVDGLHAASH HALANTGLIP SDPAKVDFIK GNVKARARMV
160 170 180 190 200
AQYEIAGYVG GLVLGTDHSA ENITGFYTKF GDGACDLAPL FGLNKRQVRL
210 220 230 240 250
LAKTLGAPEQ LVYKTPTADL EELAPQKADE AALNLTYEQI DDFLEGKAVP
260 270
AEVSQRLVAI YHATQHKRQP IPTIYD
Length:276
Mass (Da):30,403
Last modified:October 1, 2000 - v1
Checksum:i94F016DE81052A89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA. Translation: AAF96119.1.
PIRiH82488.
RefSeqiNP_232606.1. NC_002506.1.
WP_000400328.1. NC_002506.1.

Genome annotation databases

EnsemblBacteriaiAAF96119; AAF96119; VC_A0207.
GeneIDi2612343.
KEGGivch:VCA0207.
PATRICi20084981. VBIVibCho83274_2842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA. Translation: AAF96119.1.
PIRiH82488.
RefSeqiNP_232606.1. NC_002506.1.
WP_000400328.1. NC_002506.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3Q4GX-ray2.40A/B1-276[»]
ProteinModelPortaliQ9KMW1.
SMRiQ9KMW1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VCA0207.

Protocols and materials databases

DNASUi2612343.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF96119; AAF96119; VC_A0207.
GeneIDi2612343.
KEGGivch:VCA0207.
PATRICi20084981. VBIVibCho83274_2842.

Phylogenomic databases

eggNOGiENOG4107RA1. Bacteria.
COG0171. LUCA.
KOiK01916.
OMAiAKIIEGW.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.
BioCyciVCHO:VCA0207-MONOMER.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADE_VIBCH
AccessioniPrimary (citable) accession number: Q9KMW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.