ID   Q9KMS0_VIBCH            Unreviewed;       466 AA.
AC   Q9KMS0;
DT   01-OCT-2000, integrated into UniProtKB/TrEMBL.
DT   01-OCT-2000, sequence version 1.
DT   27-MAR-2024, entry version 125.
DE   RecName: Full=Alpha-amylase {ECO:0000256|RuleBase:RU361134};
DE            EC=3.2.1.1 {ECO:0000256|RuleBase:RU361134};
GN   OrderedLocusNames=VC_A0250 {ECO:0000313|EMBL:AAF96161.1};
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277 {ECO:0000313|EMBL:AAF96161.1, ECO:0000313|Proteomes:UP000000584};
RN   [1] {ECO:0000313|EMBL:AAF96161.1, ECO:0000313|Proteomes:UP000000584}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961
RC   {ECO:0000313|Proteomes:UP000000584};
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D., Ermolaeva M.D.,
RA   Vamathevan J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.,
RA   Utterback T., Fleishmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in
CC         polysaccharides containing three or more (1->4)-alpha-linked D-
CC         glucose units.; EC=3.2.1.1; Evidence={ECO:0000256|RuleBase:RU361134};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061, ECO:0000256|RuleBase:RU003615}.
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DR   EMBL; AE003853; AAF96161.1; -; Genomic_DNA.
DR   PIR; B82482; B82482.
DR   RefSeq; NP_232648.1; NC_002506.1.
DR   RefSeq; WP_000842853.1; NZ_LT906615.1.
DR   AlphaFoldDB; Q9KMS0; -.
DR   SMR; Q9KMS0; -.
DR   STRING; 243277.VC_A0250; -.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   DNASU; 2612181; -.
DR   EnsemblBacteria; AAF96161; AAF96161; VC_A0250.
DR   KEGG; vch:VC_A0250; -.
DR   PATRIC; fig|243277.26.peg.2883; -.
DR   eggNOG; COG0366; Bacteria.
DR   HOGENOM; CLU_029247_2_0_6; -.
DR   Proteomes; UP000000584; Chromosome 2.
DR   GO; GO:0004556; F:alpha-amylase activity; IBA:GO_Central.
DR   GO; GO:0043169; F:cation binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd11315; AmyAc_bac1_AmyA; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR006046; Alpha_amylase.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR   PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PRINTS; PR00110; ALPHAAMYLASE.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|RuleBase:RU361134};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361134};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361134};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000584};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           24..466
FT                   /note="Alpha-amylase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004329756"
FT   DOMAIN          24..382
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   466 AA;  52573 MW;  7CABB77330C05D3F CRC64;
     MKSSYVVLCA TFAAASFTSS VQAEAILHAF NWKYADVTAN AAQIAAAGYK KVLISPALKS
     SGNEWWARYQ PQDLRVIDSP LGNKTDLQTL IKTLQDYGVE VYSDVVFNHM ANESWKRSDL
     NYPGSEVLAS YTNQAYYYQQ QKLFGDLSHN LFSQNDFHPE GCITDWGNPG HVQYWRLCGG
     AGDRGLPDLD ANAWVINQQR AYLQALKQMG IRGFRIDAVK HMSQYHIDQV FTPEIISGMH
     VFGEVITSGG QGDGSYDAFL APYLNNTTHA AYDFPLFASI RAAFSYQGSM SQLHDPQAYG
     QALPNARAIT FAITHDIPTN DGFRYQILNP QDEQLAYAYL LGKDGGTPLI YSDDLADHED
     KDNGRWANVW NQPSMQHMLR FHNAMQGKPM TVMYSDSCLM LFKRGKEGVV GINKCGESKN
     VTIDTYQHEF NWYQTYQDTL SDDRFSVSSR YHTLTIPARV ARMWML
//