Reviewed, UniProtKB/Swiss-Prot Q9KMP4 (GLYA2_VIBCH)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 2
      Short name=Serine methylase 2
      Short name=SHMT 2
    EC=2.1.2.1

Gene names

Name:	glyA2
Ordered Locus Names:	VC_A0278

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity. HAMAP MF_00051
Subcellular location	Cytoplasm By similarity HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Serine hydroxymethyltransferase 2 HAMAP MF_00051		PRO_0000113691
Amino acid modifications
	Modified residue	244	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KMP4-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 73E55E3FBE0DF9A7	FASTA	435	46,911
	10 20 30 40 50 60 MNANLNKAYP NVSLENFFST PLAATNDAVF AAIQAEYTRQ NEQIELIASE NIVSKAVMQA 70 80 90 100 110 120 QGTCLTNKYA EGYPGRRYYG GCEHVDSVEQ IAIERAKMLF QCQYANVQPH SGAQANGAVM 130 140 150 160 170 180 LALLQPGDTI MGMSLDAGGH LTHGARPALS GKWFNAVQYG VDRQTLEINY DSVRALALEH 190 200 210 220 230 240 KPKMIIAGGS AIPRTIDFAQ FRSIVDEVGA LLMVDMAHIA GLVATGAHPS PLPHAHVVTT 250 260 270 280 290 300 TTHKTLRGPR GGMILTNSEE IHKKINSAVF PGLQGGPLMH VIAAKAVAFG EALGPEFRTY 310 320 330 340 350 360 IDSVIDNAKV LAEVLQTRGC DIVTGGTDTH LMLVDLRPKG LKGNQVEQAL ERAGITCNKN 370 380 390 400 410 420 GIPFDEEKPM ITSGIRLGTP AGTSRGFGRE EFKLIGEWIG DVLDGLVASP EGNPDVEQQV 430 RKQVKALCQR FPLYQ

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003853 Genomic DNA. Translation: AAF96188.1.
PIR	A82480.
RefSeq	NP_232675.1.
3D structure databases
SMR	Q9KMP4. Positions 22-427.
ModBase	Search...
Genome annotation databases
GeneID	2612058.
GenomeReviews	Gene locus VC_A0278 in contig AE003853_GR.
KEGG	vch:VCA0278.
TIGR	VC_A0278.
Phylogenomic databases
HOGENOM	HBG301263.
OMA	MILTNHE.
Enzyme and pathway databases
BRENDA	2.1.2.1. 19019.
Family and domain databases
HAMAP	MF_00051_B. SHMT_B. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA2_VIBCH

Accession

Primary (citable) accession number: Q9KMP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	October 1, 2000
Last modified:	January 19, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)