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Q9KMP4 (GLYA2_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase 2
Short name=SHMT 2
Short name=Serine methylase 2
EC=2.1.2.1
Gene names
Name:glyA2
Ordered Locus Names:VC_A0278
OrganismVibrio cholerae
Taxonomic identifier666 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length435 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 435435Pyridoxal-phosphate-dependent serine hydroxymethyltransferase 2
PRO_0000113691

Regions

Region139 – 1413Substrate binding By similarity

Sites

Binding site491Pyridoxal phosphate By similarity
Binding site691Pyridoxal phosphate By similarity
Binding site711Substrate By similarity
Binding site781Substrate binding By similarity
Binding site791Pyridoxal phosphate By similarity
Binding site1351Substrate By similarity
Binding site1901Pyridoxal phosphate By similarity
Binding site2181Pyridoxal phosphate By similarity
Binding site2431Pyridoxal phosphate By similarity
Binding site2501Pyridoxal phosphate By similarity
Binding site2761Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3761Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2441N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KMP4 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 73E55E3FBE0DF9A7

FASTA43546,911
        10         20         30         40         50         60 
MNANLNKAYP NVSLENFFST PLAATNDAVF AAIQAEYTRQ NEQIELIASE NIVSKAVMQA 

        70         80         90        100        110        120 
QGTCLTNKYA EGYPGRRYYG GCEHVDSVEQ IAIERAKMLF QCQYANVQPH SGAQANGAVM 

       130        140        150        160        170        180 
LALLQPGDTI MGMSLDAGGH LTHGARPALS GKWFNAVQYG VDRQTLEINY DSVRALALEH 

       190        200        210        220        230        240 
KPKMIIAGGS AIPRTIDFAQ FRSIVDEVGA LLMVDMAHIA GLVATGAHPS PLPHAHVVTT 

       250        260        270        280        290        300 
TTHKTLRGPR GGMILTNSEE IHKKINSAVF PGLQGGPLMH VIAAKAVAFG EALGPEFRTY 

       310        320        330        340        350        360 
IDSVIDNAKV LAEVLQTRGC DIVTGGTDTH LMLVDLRPKG LKGNQVEQAL ERAGITCNKN 

       370        380        390        400        410        420 
GIPFDEEKPM ITSGIRLGTP AGTSRGFGRE EFKLIGEWIG DVLDGLVASP EGNPDVEQQV 

       430 
RKQVKALCQR FPLYQ

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE003853 Genomic DNA. Translation: AAF96188.1.
PIRA82480.
RefSeqNP_232675.1. NC_002506.1.

3D structure databases

ProteinModelPortalQ9KMP4.
SMRQ9KMP4. Positions 22-427.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2612058.
GenomeReviewsGene locus VC_A0278 in contig AE003853_GR.
KEGGvch:VCA0278.
PATRIC20085121. VBIVibCho83274_2912.
TIGRVC_A0278.

Phylogenomic databases

HOGENOMHBG301263.
OMAMILTNHE.
PhylomeDBQ9KMP4.
ProtClustDBPRK13034.

Family and domain databases

HAMAPMF_00051. SHMT.
[Tree]
InterProIPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00600.
PANTHERPTHR11680. Gly_HO-Metrfase. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA2_VIBCH
AccessionPrimary (citable) accession number: Q9KMP4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 1, 2000
Last modified: January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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