Reviewed, UniProtKB/Swiss-Prot Q9KM66 (CQSS_VIBCH)

Names and origin

Protein names

Recommended name:
    CAI-1 autoinducer sensor kinase/phosphatase cqsS
    EC=2.7.13.3
    EC=3.1.3.-
Alternative name(s):
    Cholerae quorum-sensing sensor

Gene names

Name:	cqsS
Ordered Locus Names:	VC_A0522

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Senses the quorum-sensing autoinducer CAI-1 ((S)-3-hydroxytridecan-4-one) which probably functions as an intragenus signal. The sensory signal is then relayed to luxU and luxO. Ref.2
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Contains 1 histidine kinase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW two-component response regulator activity Inferred from electronic annotation. Source: InterPro two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 686	686	CAI-1 autoinducer sensor kinase/phosphatase cqsS		PRO_0000316891
Regions
	Transmembrane	21 – 41	21	Potential
	Transmembrane	47 – 64	18	Potential
	Transmembrane	77 – 97	21	Potential
	Transmembrane	100 – 120	21	Potential
	Transmembrane	124 – 144	21	Potential
	Transmembrane	152 – 172	21	Potential
	Domain	191 – 416	226	Histidine kinase
	Domain	569 – 686	118	Response regulatory
Amino acid modifications
	Modified residue	194	1	Phosphohistidine; by autocatalysis By similarity
	Modified residue	618	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KM66-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 0662BF71AF3B9562	FASTA	686	78,382
	10 20 30 40 50 60 MIVSMDVIKR VYQYAEPNLS LVGWMGMLGF PAYYFIWEYW FPQSYENLGL RCAAAVLFGG 70 80 90 100 110 120 LVFRDSMPKK WQRYMPGYFL FTIGFCLPFF FAFMMLMNDW STIWAMSFMA SIFLHILLVH 130 140 150 160 170 180 DTRVMALQAL FSVLVAYLAV YGLTDFHPTT LIEWQYIPIF LFTYVFGNLC FFRNQISHET 190 200 210 220 230 240 KVSIAKTFGA GIAHEMRNPL SALKTSIDVV RTMIPKPQTA AHTDYSLDAQ ELDLLHQILN 250 260 270 280 290 300 EADDVIYSGN NAIDLLLTSI DENRVSPASF KKHSVVDVIE KAVKTFPYKN AADQHSVELE 310 320 330 340 350 360 VHQPFDFFGS DTLLTYALFN LLKNAFYYQK EHFSVCISIE QTSEHNLIRV RDNGVGIAPE 370 380 390 400 410 420 MLEDIFRDFY TFGKNGSYGL GLPFCRKVMS AFGGTIRCAS QQGQWTEFVL SFPRYDSDTV 430 440 450 460 470 480 NEIKTELLKT KSLIYIGSNQ AIVRELNQLA VEDEFGFTAI SAQQAVRRQD YEFEFDLILL 490 500 510 520 530 540 DLDDATAQGE LLPKLEGTLS FAEGCIGYVY DPGKTYAVNI NRYLRIQPIS IHSILRKPRK 550 560 570 580 590 600 IIERLLFEQE SLSMNRNVIP LQKSRHERRI LVVDDNQSIR TFTAILLEQQ GYEVVQANDG 610 620 630 640 650 660 SEVLKHMESQ NIDLVLMDIE MPNVGGLEAT RLIRNSEHEY KNIPIIGYTG DNSPKTLALV 670 680 QTSGMNDFIV KPADRDVLLN KVAAWV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.
[2]	"Parallel quorum sensing systems converge to regulate virulence in Vibrio cholerae." Miller M.B., Skorupski K., Lenz D.H., Taylor R.K., Bassler B.L. Cell 110:303-314(2002) [PubMed: 12176318] [Abstract] Cited for: FUNCTION AS THE CAI-1 SENSOR.
[3]	"The major Vibrio cholerae autoinducer and its role in virulence factor production." Higgins D.A., Pomianek M.E., Kraml C.M., Taylor R.K., Semmelhack M.F., Bassler B.L. Nature 450:883-886(2007) [PubMed: 18004304] [Abstract] Cited for: IDENTIFICATION OF CAI-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE003853 Genomic DNA. Translation: AAF96425.1.
PIR	G82448.
RefSeq	NP_232913.1.
3D structure databases
HSSP	HSSP built from PDB template 1JBE based on UniProtKB P06143.
ModBase	Search...
Genome annotation databases
GeneID	2612235.
GenomeReviews	Gene locus VC_A0522 in contig AE003853_GR.
KEGG	vch:VCA0522.
TIGR	VC_A0522.
Phylogenomic databases
HOGENOM	Q9KM66.
OMA	GLGLPFC.
Enzyme and pathway databases
BRENDA	2.7.13.3. 19019.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF02518. HATPase_c. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
ProDom	PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50109. HIS_KIN. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CQSS_VIBCH

Accession

Primary (citable) accession number: Q9KM66

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)