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Protein

CAI-1 autoinducer synthase

Gene

cqsA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the synthesis of the quorum-sensing autoinducer CAI-1 ((S)-3-hydroxytridecan-4-one) which probably functions as an intragenus signal.1 Publication

Cofactori

GO - Molecular functioni

  • pyridoxal phosphate binding Source: InterPro
  • transaminase activity Source: TIGR
  • transferase activity, transferring acyl groups Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-19105.
VCHO:VCA0523-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CAI-1 autoinducer synthase (EC:2.3.-.-)
Alternative name(s):
Cholerae quorum-sensing autoinducer
Gene namesi
Name:cqsA
Ordered Locus Names:VC_A0523
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003168991 – 389CAI-1 autoinducer synthaseAdd BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei236N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Protein-protein interaction databases

STRINGi243277.VCA0523.

Structurei

Secondary structure

1389
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 21Combined sources14
Turni22 – 24Combined sources3
Beta strandi41 – 43Combined sources3
Helixi56 – 67Combined sources12
Helixi77 – 80Combined sources4
Turni83 – 85Combined sources3
Helixi88 – 97Combined sources10
Beta strandi100 – 106Combined sources7
Helixi108 – 119Combined sources12
Beta strandi125 – 129Combined sources5
Helixi134 – 142Combined sources9
Beta strandi146 – 150Combined sources5
Helixi155 – 165Combined sources11
Beta strandi169 – 175Combined sources7
Turni177 – 179Combined sources3
Helixi185 – 195Combined sources11
Beta strandi198 – 202Combined sources5
Turni204 – 209Combined sources6
Helixi212 – 214Combined sources3
Helixi217 – 220Combined sources4
Helixi224 – 226Combined sources3
Beta strandi228 – 236Combined sources9
Beta strandi243 – 250Combined sources8
Helixi251 – 253Combined sources3
Helixi255 – 258Combined sources4
Helixi260 – 264Combined sources5
Helixi270 – 285Combined sources16
Helixi287 – 305Combined sources19
Turni306 – 308Combined sources3
Beta strandi314 – 324Combined sources11
Helixi325 – 337Combined sources13
Beta strandi343 – 345Combined sources3
Turni347 – 349Combined sources3
Beta strandi355 – 360Combined sources6
Helixi367 – 383Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WK7X-ray2.50A/B1-389[»]
2WK8X-ray2.10A/B1-389[»]
2WK9X-ray1.90A/B1-389[»]
2WKAX-ray1.91A/B1-389[»]
3HQTX-ray2.70A/B1-389[»]
3KKIX-ray1.80A/B1-389[»]
ProteinModelPortaliQ9KM65.
SMRiQ9KM65.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KM65.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107KBT. Bacteria.
COG0156. LUCA.
KOiK10915.
OMAiYSTIGTI.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KM65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKPQLPDFI QNKIDHYIEN YFDINKNGKH LVLGKQASPD DIILQSNDYL
60 70 80 90 100
ALANHPLIKA RLAKSLLEEQ QSLFMSASFL QNDYDKPMIE KRLAKFTGFD
110 120 130 140 150
ECLLSQSGWN ANVGLLQTIC QPNTNVYIDF FAHMSLWEGA RYANAQAHPF
160 170 180 190 200
MHNNCDHLRM LIQRHGPGII VVDSIYSTLG TIAPLAELVN ISKEFGCALL
210 220 230 240 250
VDESHSLGTH GPNGAGLLAE LGLTREVHFM TASLAKTFAY RAGAIWCNNE
260 270 280 290 300
VNRCVPFISY PAIFSSTLLP YEAAGLETTL EIIESADNRR QHLDRMARKL
310 320 330 340 350
RIGLSQLGLT IRSESQIIGL ETGDERNTEK VRDYLESNGV FGSVFCRPAT
360 370 380
SKNKNIIRLS LNSDVNDEQI AKIIEVCSDA VNYGDFYFR
Length:389
Mass (Da):43,594
Last modified:October 1, 2000 - v1
Checksum:iAB37F11195D54C74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA. Translation: AAF96426.1.
PIRiH82448.
RefSeqiNP_232914.1. NC_002506.1.
WP_001039912.1. NC_002506.1.

Genome annotation databases

EnsemblBacteriaiAAF96426; AAF96426; VC_A0523.
GeneIDi2612753.
KEGGivch:VCA0523.
PATRICi20085596. VBIVibCho83274_3149.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA. Translation: AAF96426.1.
PIRiH82448.
RefSeqiNP_232914.1. NC_002506.1.
WP_001039912.1. NC_002506.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WK7X-ray2.50A/B1-389[»]
2WK8X-ray2.10A/B1-389[»]
2WK9X-ray1.90A/B1-389[»]
2WKAX-ray1.91A/B1-389[»]
3HQTX-ray2.70A/B1-389[»]
3KKIX-ray1.80A/B1-389[»]
ProteinModelPortaliQ9KM65.
SMRiQ9KM65.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VCA0523.

Protocols and materials databases

DNASUi2612753.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF96426; AAF96426; VC_A0523.
GeneIDi2612753.
KEGGivch:VCA0523.
PATRICi20085596. VBIVibCho83274_3149.

Phylogenomic databases

eggNOGiENOG4107KBT. Bacteria.
COG0156. LUCA.
KOiK10915.
OMAiYSTIGTI.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-19105.
VCHO:VCA0523-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9KM65.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCQSA_VIBCH
AccessioniPrimary (citable) accession number: Q9KM65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.