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Protein

CAI-1 autoinducer synthase

Gene

cqsA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the synthesis of the quorum-sensing autoinducer CAI-1 ((S)-3-hydroxytridecan-4-one) which probably functions as an intragenus signal.1 Publication

Cofactori

GO - Molecular functioni

  • pyridoxal phosphate binding Source: InterPro
  • transaminase activity Source: TIGR
  • transferase activity, transferring acyl groups Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciVCHO:VCA0523-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CAI-1 autoinducer synthase (EC:2.3.-.-)
Alternative name(s):
Cholerae quorum-sensing autoinducer
Gene namesi
Name:cqsA
Ordered Locus Names:VC_A0523
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 389389CAI-1 autoinducer synthasePRO_0000316899Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei236 – 2361N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Protein-protein interaction databases

STRINGi243277.VCA0523.

Structurei

Secondary structure

1
389
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 2114Combined sources
Turni22 – 243Combined sources
Beta strandi41 – 433Combined sources
Helixi56 – 6712Combined sources
Helixi77 – 804Combined sources
Turni83 – 853Combined sources
Helixi88 – 9710Combined sources
Beta strandi100 – 1067Combined sources
Helixi108 – 11912Combined sources
Beta strandi125 – 1295Combined sources
Helixi134 – 1429Combined sources
Beta strandi146 – 1505Combined sources
Helixi155 – 16511Combined sources
Beta strandi169 – 1757Combined sources
Turni177 – 1793Combined sources
Helixi185 – 19511Combined sources
Beta strandi198 – 2025Combined sources
Turni204 – 2096Combined sources
Helixi212 – 2143Combined sources
Helixi217 – 2204Combined sources
Helixi224 – 2263Combined sources
Beta strandi228 – 2369Combined sources
Beta strandi243 – 2508Combined sources
Helixi251 – 2533Combined sources
Helixi255 – 2584Combined sources
Helixi260 – 2645Combined sources
Helixi270 – 28516Combined sources
Helixi287 – 30519Combined sources
Turni306 – 3083Combined sources
Beta strandi314 – 32411Combined sources
Helixi325 – 33713Combined sources
Beta strandi343 – 3453Combined sources
Turni347 – 3493Combined sources
Beta strandi355 – 3606Combined sources
Helixi367 – 38317Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WK7X-ray2.50A/B1-389[»]
2WK8X-ray2.10A/B1-389[»]
2WK9X-ray1.90A/B1-389[»]
2WKAX-ray1.91A/B1-389[»]
3HQTX-ray2.70A/B1-389[»]
3KKIX-ray1.80A/B1-389[»]
ProteinModelPortaliQ9KM65.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KM65.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107KBT. Bacteria.
COG0156. LUCA.
KOiK10915.
OMAiIFGAVFC.
OrthoDBiEOG6Q8HZD.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KM65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKPQLPDFI QNKIDHYIEN YFDINKNGKH LVLGKQASPD DIILQSNDYL
60 70 80 90 100
ALANHPLIKA RLAKSLLEEQ QSLFMSASFL QNDYDKPMIE KRLAKFTGFD
110 120 130 140 150
ECLLSQSGWN ANVGLLQTIC QPNTNVYIDF FAHMSLWEGA RYANAQAHPF
160 170 180 190 200
MHNNCDHLRM LIQRHGPGII VVDSIYSTLG TIAPLAELVN ISKEFGCALL
210 220 230 240 250
VDESHSLGTH GPNGAGLLAE LGLTREVHFM TASLAKTFAY RAGAIWCNNE
260 270 280 290 300
VNRCVPFISY PAIFSSTLLP YEAAGLETTL EIIESADNRR QHLDRMARKL
310 320 330 340 350
RIGLSQLGLT IRSESQIIGL ETGDERNTEK VRDYLESNGV FGSVFCRPAT
360 370 380
SKNKNIIRLS LNSDVNDEQI AKIIEVCSDA VNYGDFYFR
Length:389
Mass (Da):43,594
Last modified:October 1, 2000 - v1
Checksum:iAB37F11195D54C74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA. Translation: AAF96426.1.
PIRiH82448.
RefSeqiNP_232914.1. NC_002506.1.
WP_001039912.1. NC_002506.1.

Genome annotation databases

EnsemblBacteriaiAAF96426; AAF96426; VC_A0523.
GeneIDi2612753.
KEGGivch:VCA0523.
PATRICi20085596. VBIVibCho83274_3149.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA. Translation: AAF96426.1.
PIRiH82448.
RefSeqiNP_232914.1. NC_002506.1.
WP_001039912.1. NC_002506.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WK7X-ray2.50A/B1-389[»]
2WK8X-ray2.10A/B1-389[»]
2WK9X-ray1.90A/B1-389[»]
2WKAX-ray1.91A/B1-389[»]
3HQTX-ray2.70A/B1-389[»]
3KKIX-ray1.80A/B1-389[»]
ProteinModelPortaliQ9KM65.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VCA0523.

Protocols and materials databases

DNASUi2612753.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF96426; AAF96426; VC_A0523.
GeneIDi2612753.
KEGGivch:VCA0523.
PATRICi20085596. VBIVibCho83274_3149.

Phylogenomic databases

eggNOGiENOG4107KBT. Bacteria.
COG0156. LUCA.
KOiK10915.
OMAiIFGAVFC.
OrthoDBiEOG6Q8HZD.

Enzyme and pathway databases

BioCyciVCHO:VCA0523-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9KM65.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.
  2. "Parallel quorum sensing systems converge to regulate virulence in Vibrio cholerae."
    Miller M.B., Skorupski K., Lenz D.H., Taylor R.K., Bassler B.L.
    Cell 110:303-314(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS THE CAI-1 SYNTHASE.
  3. "The major Vibrio cholerae autoinducer and its role in virulence factor production."
    Higgins D.A., Pomianek M.E., Kraml C.M., Taylor R.K., Semmelhack M.F., Bassler B.L.
    Nature 450:883-886(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF CAI-1.

Entry informationi

Entry nameiCQSA_VIBCH
AccessioniPrimary (citable) accession number: Q9KM65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 1, 2000
Last modified: November 11, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.