Q9KLX7 (FTHS_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	VC_A0614

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1 › Vibrio cholerae O1 biovar El Tor

Protein attributes

Sequence length	582 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.
Sequence caution	The sequence AAF96515.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro histidine biosynthetic process Inferred from sequence or structural similarity Ref.1. Source: TIGR methionine biosynthetic process Inferred from sequence or structural similarity Ref.1. Source: TIGR one-carbon metabolic process Inferred from sequence or structural similarity Ref.1. Source: TIGR pantothenate biosynthetic process Inferred from sequence or structural similarity Ref.1. Source: TIGR purine nucleobase biosynthetic process Inferred from sequence or structural similarity Ref.1. Source: TIGR tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from sequence or structural similarity Ref.1. Source: TIGR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 582	582	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000199407
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KLX7 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 51C920CE75F6E1EC	FASTA	582	62,074
	10 20 30 40 50 60 MLPDIEICRA TPLAPIDTIA QKAGLHANEY ESHGQHKAKV SLHCLERLAN KPKGKFILVT 70 80 90 100 110 120 AITPTPLGEG KTVTTIGLAQ GLAKLNHSVM ACIRQPSMGP IFGVKGGAAG GGYSQVAPME 130 140 150 160 170 180 ELNLHLTGDI HAVTAAHNLA AAAIDARIYH EQRLGYADFE RRTGMPALRI DPKQVIWKRV 190 200 210 220 230 240 MDHNDRALRM VTVGRNEPGK NINGYEREDG FDISAASELM AILALASDLR DLRRRIGNVV 250 260 270 280 290 300 LAYDLDGNPV TTEDLKVAGA MAVSMKEAIE PTLMQTLEGV PTLIHAGPFA NIAHGNSSII 310 320 330 340 350 360 ADEIATRLAD YTVTEGGFGS DMGFEKACNI KAKASGKTPD CAVIVATLRG LKANSGLYDL 370 380 390 400 410 420 RPGQAVPDAL FAPDSAALQA GFANLKWHID NVNQYGVPAV VAINRFPQDC AEELEQLVKL 430 440 450 460 470 480 IEALPNRVSV AISEGFAKGG EGTKLLAEKV VEQCQHPTKF TPLYDSGIPL DEKLKAVAVK 490 500 510 520 530 540 GYGAAEIALS DKAAQQLAKL QTQGFDHLAV CLAKTPLSIS TDPAIKGAPR DFIVPIRELR 550 560 570 580 LCAGAEFVYA LCGSVMTMPG LPEKPSFMAL DIDQHGNIVG LS

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003853 Genomic DNA. Translation: AAF96515.1. Different initiation.
PIR	B82439.
RefSeq	NP_233003.2. NC_002506.1.
3D structure databases
ProteinModelPortal	Q9KLX7.
ModBase	Search...
Protein-protein interaction databases
STRING	243277.VCA0614.
Protocols and materials databases
DNASU	2612837.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF96515; AAF96515; VC_A0614.
GeneID	2612837.
KEGG	vch:VCA0614.
PATRIC	20085784. VBIVibCho83274_3242.
Phylogenomic databases
eggNOG	COG2759.
KO	K01938.
OMA	AIKPNIM.
ProtClustDB	PRK13506.
Enzyme and pathway databases
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_VIBCH

Accession

Primary (citable) accession number: Q9KLX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	May 1, 2013
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program