Reviewed, UniProtKB/Swiss-Prot Q9KLX6 (MSRAB_VIBCH)

Names and origin

Protein names

Recommended name:
    Peptide methionine sulfoxide reductase msrA/msrB
Including the following 2 domains:
    1- Recommended name:
            Peptide methionine sulfoxide reductase msrA
                Short name=Protein-methionine-S-oxide reductase
              EC=1.8.4.11
        Alternative name(s):
            Peptide-methionine (S)-S-oxide reductase
              Short name=Peptide Met(O) reductase
    2- Recommended name:
            Peptide methionine sulfoxide reductase msrB
              EC=1.8.4.12
        Alternative name(s):
            Peptide-methionine (R)-S-oxide reductase

Gene names

Name:	msrAB
Ordered Locus Names:	VC_A0615

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine By similarity.
Catalytic activity	Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin. HAMAP MF_01400 L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin. HAMAP MF_01400 Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin.
Sequence similarities	In the N-terminal section; belongs to the msrA Met sulfoxide reductase family. In the C-terminal section; belongs to the msrB Met sulfoxide reductase family.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein modification process Inferred from electronic annotation. Source: HAMAP
Molecular function	peptide-methionine (R)-S-oxide reductase activity Inferred from electronic annotation. Source: EC peptide-methionine-(S)-S-oxide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Peptide methionine sulfoxide reductase msrA/msrB HAMAP MF_01400		PRO_0000138526
Regions
	Region	40 – 197	158	Peptide methionine sulfoxide reductase A HAMAP MF_01400
	Region	240 – 362	123	Peptide methionine sulfoxide reductase B HAMAP MF_01400
Sites
	Active site	48	1	By similarity
	Active site	351	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KLX6-1 [UniParc]. Last modified May 2, 2002. Version 2. Checksum: BEE0F1A614562B10	FASTA	378	43,477
	10 20 30 40 50 60 MKRLFAWGAP LFALVALTLS LFSQADTKPS TIDSTSANYQ QATLAGGCFW CTESDMEKLP 70 80 90 100 110 120 GVVDVISGYA GGDVDNPTYK QVSSGKTGHI EVIQVTFDPK IVTYEQVLDN FFRHIDPTDD 130 140 150 160 170 180 QGSFVDRGEQ YRPAIFYHNA EQLEVAKRFM MEIDQLGIFK KPLKTELIEF KKFWPAEDYH 190 200 210 220 230 240 QDYYKKNKVR YNYYRYASGR DQYLDEIFGA DRNTHPKTLR QWIDEKNGQA NVKAYVRPSD 250 260 270 280 290 300 EQIRAKLTSL QYKVTQRDGT ERPFDNEYWD NKEEGIYVDI VSGEPLFSST DKYDSKTGWP 310 320 330 340 350 360 SFTQPLNSSY IVTKDDNSLF YTRTEVRSRF ADSHLGHVFN DGPAPTGLRY CMNSAAMRFI 370 PKQEMAAQGY GEYLALFK

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE003853 Genomic DNA. Translation: AAF96516.1. Different initiation.
PIR	C82439.
RefSeq	NP_233004.1.
3D structure databases
HSSP	HSSP built from PDB template 1L1D based on UniProtKB P14930.
ModBase	Search...
Genome annotation databases
GeneID	2612594.
GenomeReviews	Gene locus VC_A0615 in contig AE003853_GR.
KEGG	vch:VCA0615.
TIGR	VC_A0615.
Phylogenomic databases
HOGENOM	Q9KLX6.
Enzyme and pathway databases
BRENDA	1.8.4.11. 19019. 1.8.4.12. 19019.
Family and domain databases
HAMAP	MF_01400. Fused. [Tree] MF_01401. Fused. [Tree]
InterPro	IPR002579. Methionine_sulphoxide_MsrB. IPR002569. MsrA. [Graphical view]
Gene3D	G3DSA:3.30.1060.10. MsrA. 1 hit. G3DSA:2.170.150.20. MsrB. 1 hit.
Pfam	PF01625. PMSR. 1 hit. PF01641. SelR. 1 hit. [Graphical view]
ProDom	PD004057. DUF25. 1 hit. PD003489. PMSR. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00401. msrA. 1 hit. TIGR00357. MsrB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MSRAB_VIBCH

Accession

Primary (citable) accession number: Q9KLX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	May 2, 2002
Last modified:	March 3, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)