Q9KLX5 (GCH1_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP cyclohydrolase 1
EC=3.5.4.16

Alternative name(s):
GTP cyclohydrolase I
Short name=GTP-CH-I

Gene names

Name:	folE
Ordered Locus Names:	VC_A0616

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1 › Vibrio cholerae O1 biovar El Tor

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate. HAMAP-Rule MF_00223
Pathway	Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1. HAMAP-Rule MF_00223
Subunit structure	Toroid-shaped homodecamer, composed of two pentamers of five dimers By similarity.
Sequence similarities	Belongs to the GTP cyclohydrolase I family.
Sequence caution	The sequence AAF96517.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	GTP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	7,8-dihydroneopterin 3'-triphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway folic acid-containing compound metabolic process Inferred from sequence or structural similarity Ref.1. Source: TIGR one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase I activity Inferred from sequence or structural similarity Ref.1. Source: TIGR zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	GTP cyclohydrolase 1 HAMAP-Rule MF_00223		PRO_0000119460
Sites
	Metal binding	109	1	Zinc By similarity
	Metal binding	112	1	Zinc By similarity
	Metal binding	180	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KLX5 [UniParc]. Last modified October 10, 2002. Version 2. Checksum: D5D3E776BD633213	FASTA	217	24,143
	10 20 30 40 50 60 MSGLSESAKL VKDALERRGL ETPMQPNLAT PAEKKEKIEQ HMREILNLLG LDLTDDSLEE 70 80 90 100 110 120 TPQRIAKMYV DEIFSGLDYA NFPKITVIEN KMKVSEMVKV KDITLTSTCE HHLVTIDGTA 130 140 150 160 170 180 AVAYIPRGKI IGLSKINRIV RFFAQRPQVQ ERMTQQILVA LQTLLESDDV AVTIDATHYC 190 200 210 VKSRGVMDAT SVTTTTALGG IFKSNPATRA EFLHGLR

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003853 Genomic DNA. Translation: AAF96517.1. Different initiation.
PIR	D82439.
RefSeq	NP_233005.1. NC_002506.1.
3D structure databases
ProteinModelPortal	Q9KLX5.
SMR	Q9KLX5. Positions 4-217.
ModBase	Search...
Protein-protein interaction databases
STRING	243277.VCA0616.
Proteomic databases
PRIDE	Q9KLX5.
Protocols and materials databases
DNASU	2612591.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF96517; AAF96517; VC_A0616.
GeneID	2612591.
KEGG	vch:VCA0616.
PATRIC	20085788. VBIVibCho83274_3244.
Phylogenomic databases
eggNOG	COG0302.
KO	K01495.
OMA	PTRIAKM.
ProtClustDB	PRK09347.
Enzyme and pathway databases
UniPathway	UPA00848; UER00151.
Family and domain databases
HAMAP	MF_00223. FolE.
InterPro	IPR001474. GTP_CycHdrlase_I. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. IPR018234. GTP_CycHdrlase_I_CS. [Graphical view]
PANTHER	PTHR11109. PTHR11109. 1 hit.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
TIGRFAMs	TIGR00063. folE. 1 hit.
PROSITE	PS00859. GTP_CYCLOHYDROL_1_1. 1 hit. PS00860. GTP_CYCLOHYDROL_1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCH1_VIBCH

Accession

Primary (citable) accession number: Q9KLX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	October 10, 2002
Last modified:	May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program