Q9KLT6 (SCRB1_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable sucrose-6-phosphate hydrolase
Short name=Sucrase
EC=3.2.1.26

Alternative name(s):
Invertase

Gene names

Ordered Locus Names:

VC_A0655

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Enables the bacterium to metabolize sucrose as a sole carbon source By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	Probable sucrose-6-phosphate hydrolase		PRO_0000341297
Regions
	Region	105 – 108	4	Substrate binding By similarity
	Region	167 – 168	2	Substrate binding By similarity
	Region	228 – 229	2	Substrate binding By similarity
Sites
	Active site	108	1	By similarity
	Binding site	124	1	Substrate By similarity
	Binding site	283	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KLT6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: EFA5328C3B66E03D	FASTA	548	62,513
	10 20 30 40 50 60 MLLDTLLELA GGINNVTRIL APQGQVVLAL KHPPLVPHLP DDVSLQSVLG EWQLSVQRTA 70 80 90 100 110 120 EVSDQQLAAI GKAIAERQKL ETLPYQTALD CPYRPLWHIS PPQGLLNDPN GFIYHQGEYH 130 140 150 160 170 180 LFYQWHPFAC EHKDKYWVHL KSLDLVDWQW QSVALTPSDW FDSHGVFSGH AVSHQQDLWL 190 200 210 220 230 240 FYTGNTRLGV DRQRQTMQCA ARMNANGEFE KLGPVIRCLP EGVTEHIRDP KVIYTQGKWQ 250 260 270 280 290 300 MLLGAQTLAH QGRLAVYHSD DLLHWHFDKL YGDELGDYGY MWECPDWFEL QGEAFFVFGP 310 320 330 340 350 360 QGIASANPHH TIEHQNRIFR ATQNAQGEIA LLQGWPLDEG FDFYAPQTAQ TADGRRVLCG 370 380 390 400 410 420 WMGLPDETQH PSCDQGWIHQ LTALRELEWR EGRIYQHPLR ELDTLQSEPH TLLLSDNVTE 430 440 450 460 470 480 LKTKSFALQV TLPWGCELRL MQNTQYRVTL TLDAENQLLR LDRSATQIRQ GDTIRELKLD 490 500 510 520 530 540 SPTVELRILA DQSSLEIFIN QGEHVMTSRI FTPRDASGIS LHGASVDAKL YYMAPASAPF NLEVNVQP

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003853 Genomic DNA. Translation: AAF96556.1.
PIR	H82432.
RefSeq	NP_233044.1. NC_002506.1.
3D structure databases
ProteinModelPortal	Q9KLT6.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2612580.
GenomeReviews	Gene locus VC_A0655 in contig AE003853_GR.
KEGG	vch:VCA0655.
PATRIC	20085866. VBIVibCho83274_3283.
TIGR	VC_A0655.
Phylogenomic databases
HOGENOM	HBG693902.
OMA	HLFYQWF.
PhylomeDB	Q9KLT6.
ProtClustDB	CLSK788970.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_43_beta-prop. IPR006232. Suc6P_hydrolase. [Graphical view]
Gene3D	G3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 1 hit.
KO	K01193.
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
TIGRFAMs	TIGR01322. ScrB_fam. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRB1_VIBCH

Accession

Primary (citable) accession number: Q9KLT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program