Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Autoinducer 2 sensor kinase/phosphatase LuxQ

Gene

luxQ

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

At low cell density, in absence of AI-2 (autoinducer 2), LuxQ has a kinase activity and autophosphorylates on a histidine residue. The phosphoryl group is then transferred to an aspartate residue in the response regulator domain. The phosphoryl group is transferred to LuxU, and ultimately to LuxO. At high cell density, in the presence of AI-2, the kinase activity is inactivated, and the response regulator domain has a phosphatase activity (By similarity).By similarity1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphoprotein phosphatase activity Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: TIGR

GO - Biological processi

  • phosphorelay signal transduction system Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Protein phosphatase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciVCHO:VCA0736-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Autoinducer 2 sensor kinase/phosphatase LuxQ (EC:2.7.13.3, EC:3.1.3.-)
Gene namesi
Name:luxQ
Ordered Locus Names:VC_A0736
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 2

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei14 – 3421HelicalSequence analysisAdd
BLAST
Transmembranei283 – 30321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 857857Autoinducer 2 sensor kinase/phosphatase LuxQPRO_0000074782Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei489 – 4891Phosphohistidine; by autocatalysisPROSITE-ProRule annotation
Modified residuei780 – 78014-aspartylphosphatePROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9KLK7.

Interactioni

Subunit structurei

Binds the complex formed by AI-2 and LuxP.By similarity

Protein-protein interaction databases

STRINGi243277.VCA0736.

Structurei

Secondary structure

1
857
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi53 – 7826Combined sources
Helixi82 – 898Combined sources
Turni93 – 953Combined sources
Helixi96 – 10510Combined sources
Helixi108 – 1103Combined sources
Beta strandi113 – 1197Combined sources
Beta strandi122 – 1265Combined sources
Helixi130 – 1334Combined sources
Helixi137 – 14610Combined sources
Beta strandi149 – 1524Combined sources
Beta strandi154 – 1585Combined sources
Beta strandi161 – 1633Combined sources
Beta strandi165 – 17410Combined sources
Beta strandi184 – 1918Combined sources
Helixi196 – 20510Combined sources
Beta strandi209 – 2157Combined sources
Beta strandi218 – 2225Combined sources
Beta strandi228 – 2303Combined sources
Helixi232 – 2365Combined sources
Beta strandi247 – 25610Combined sources
Beta strandi259 – 27012Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C30X-ray2.80A36-280[»]
3C38X-ray2.30A36-280[»]
ProteinModelPortaliQ9KLK7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KLK7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini486 – 706221Histidine kinasePROSITE-ProRule annotationAdd
BLAST
Domaini731 – 846116Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
KOiK10909.
OMAiIYACTAD.
OrthoDBiEOG6G4VQG.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR015387. LuxQ-periplasm.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF09308. LuxQ-periplasm. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
ProDomiPD142495. LuxQ-periplasm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KLK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIRPSQIKH KQRIASFITH AVVVVMGVLI VSVLFQSYQI SSRLMAQEGQ
60 70 80 90 100
RTSVQTSSLI QSLFDFRLAA LRIHQDSTAK NASLINALVS RDSSRLDEFF
110 120 130 140 150
SSVDELELSN APDLRFISSH DNILWDDGNA SFYGIAQQEL NKLIRRVAIS
160 170 180 190 200
GNWHLVQTPS EGKSVHILMR RSSLIEAGTG QVVGYLYVGI VLNDNFALLE
210 220 230 240 250
NIRSGSNSEN LVLAVDTTPL VSTLKGNEPY SLDYVVHSAK DAMRDSFIVG
260 270 280 290 300
QTFLEVESVP TYLCVYSIQT NQNVLTLRDN FYFWMAFALI SMIGVSIASR
310 320 330 340 350
WWLQKRIQRE IETLMNYTHK LMDLDTKSEF IGSKIYEFDY FGRTLEQSFR
360 370 380 390 400
RLANKEKQFE DLFNFALSPT MLWNTSGRLI RMNPSAQIQF LREDAQNHFL
410 420 430 440 450
FEILERQLLP TITNAAQGNN PSDVTTEVDG RVYRWNLSPI MVEGQIISII
460 470 480 490 500
TQGQDITTIA EAEKQSQAAR REAEESARVR AEFLAKMSHE LRTPLNGVLG
510 520 530 540 550
VSQLLKRTPL NDEQREHVAV LCSSGEHLLA VLNDILDFSR LEQGKFRIQK
560 570 580 590 600
NEFRLKELVC AIDRIYRPLC NEKGLELVVN SNITTAAIVR SDQIRINQIL
610 620 630 640 650
FNLLNNAIKF THQGSIRVEL QLIEGDPLAQ LVIQVVDTGI GIREQDLTVI
660 670 680 690 700
FEPFMQAEST TTREYGGSGL GLTIVHSLVE MLSGQLHVSS EYGIGTRFEI
710 720 730 740 750
QLPIELVEKP DAPQQLLPAP DPQPLFDKTL RVLLVEDNHT NAFIAQAFCR
760 770 780 790 800
KYGLDVSWVT DGLQAIEELK IHDYDLVLMD NQLPYLDGVE TTRTIKKVLH
810 820 830 840 850
LPVVVYACTA DGLEETRQAF FHAGAEYVLV KPLKEQTLHK ALEHFKHHHG

QKNAGLN
Length:857
Mass (Da):96,930
Last modified:October 1, 2000 - v1
Checksum:i248000A038DA5A51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA. Translation: AAF96635.1.
PIRiC82424.
RefSeqiNP_233123.1. NC_002506.1.
WP_001026306.1. NC_002506.1.

Genome annotation databases

EnsemblBacteriaiAAF96635; AAF96635; VC_A0736.
GeneIDi2611934.
KEGGivch:VCA0736.
PATRICi20086018. VBIVibCho83274_3359.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA. Translation: AAF96635.1.
PIRiC82424.
RefSeqiNP_233123.1. NC_002506.1.
WP_001026306.1. NC_002506.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C30X-ray2.80A36-280[»]
3C38X-ray2.30A36-280[»]
ProteinModelPortaliQ9KLK7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VCA0736.

Proteomic databases

PRIDEiQ9KLK7.

Protocols and materials databases

DNASUi2611934.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF96635; AAF96635; VC_A0736.
GeneIDi2611934.
KEGGivch:VCA0736.
PATRICi20086018. VBIVibCho83274_3359.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
KOiK10909.
OMAiIYACTAD.
OrthoDBiEOG6G4VQG.

Enzyme and pathway databases

BioCyciVCHO:VCA0736-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9KLK7.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR015387. LuxQ-periplasm.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF09308. LuxQ-periplasm. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
ProDomiPD142495. LuxQ-periplasm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.
  2. "Parallel quorum sensing systems converge to regulate virulence in Vibrio cholerae."
    Miller M.B., Skorupski K., Lenz D.H., Taylor R.K., Bassler B.L.
    Cell 110:303-314(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: El Tor C6706.
  3. "Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi."
    Bassler B.L., Greenberg E.P., Stevens A.M.
    J. Bacteriol. 179:4043-4045(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: AUTOINDUCER 2 (AI-2) SYNTHESIS IN V.CHOLERAE.

Entry informationi

Entry nameiLUXQ_VIBCH
AccessioniPrimary (citable) accession number: Q9KLK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2000
Last modified: July 6, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.