Reviewed, UniProtKB/Swiss-Prot Q9KLB8 (PH4H_VIBCH)

Names and origin

Protein names

Recommended name:
    Phenylalanine-4-hydroxylase
      Short name=PAH
    EC=1.14.16.1
Alternative name(s):
    Phe-4-monooxygenase

Gene names

Name:	phhA
Ordered Locus Names:	VC_A0828

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-phenylalanine + tetrahydrobiopterin + O2 = L-tyrosine + 4a-hydroxytetrahydrobiopterin.
Cofactor	Binds 1 Fe2+ ion By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 1/6.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Phenylalanine-4-hydroxylase		PRO_0000205558
Sites
	Metal binding	144	1	Iron Potential
	Metal binding	149	1	Iron Potential
	Metal binding	189	1	Iron Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9KLB8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 2D68B31C6E31D521	FASTA	289	33,445
	10 20 30 40 50 60 MSKIPLAVRI GLGRLNVTNL LRSRAMTQYH SKPVSEHGHI DWDQDEHAVW HELITRQQEV 70 80 90 100 110 120 VKTRACQAYL DGLNMLNLPT DRLPQLPEIN RVLQRETGWQ VEPVPALISF DRFFALLADK 130 140 150 160 170 180 KFPVATFLRR REEFDYLQEP DFFHEVYGHC AMLTHPDFAA FTHVYGQLGA KATPKERSYL 190 200 210 220 230 240 ARLYWFTVEF GLVQEQGQTK IYGGGILSSP GETLYASEST IPKREPFDIM QVLRTPYRID 250 260 270 280 IMQPIYYVLP DLSQLYQLSQ RDVMALVWQA MQDGLLPPLF QPKEQQHAG

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE003853 Genomic DNA. Translation: AAF96726.1.
PIR	D82413.
RefSeq	NP_233214.1.
3D structure databases
HSSP	HSSP built from PDB template 1LTU based on UniProtKB P30967.
ModBase	Search...
Genome annotation databases
GeneID	2612712.
GenomeReviews	Gene locus VC_A0828 in contig AE003853_GR.
KEGG	vch:VCA0828.
TIGR	VC_A0828.
Phylogenomic databases
HOGENOM	Q9KLB8.
OMA	Q9KLB8. FLARLYW.
Enzyme and pathway databases
BRENDA	1.14.16.1. 19019.
Family and domain databases
InterPro	IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005960. Phe-4-hydroxylase_mono. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF00351. Biopterin_H. 1 hit. [Graphical view]
PRINTS	PR00372. FYWHYDRXLASE.
ProDom	PD002559. Aaa_hydroxylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01267. Phe4hydrox_mono. 1 hit.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PH4H_VIBCH

Accession

Primary (citable) accession number: Q9KLB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)