ID   Q9KL86_VIBCH            Unreviewed;       690 AA.
AC   Q9KL86;
DT   01-OCT-2000, integrated into UniProtKB/TrEMBL.
DT   01-OCT-2000, sequence version 1.
DT   27-MAR-2024, entry version 105.
DE   SubName: Full=Alpha-amylase {ECO:0000313|EMBL:AAF96758.1};
GN   OrderedLocusNames=VC_A0860 {ECO:0000313|EMBL:AAF96758.1};
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277 {ECO:0000313|EMBL:AAF96758.1, ECO:0000313|Proteomes:UP000000584};
RN   [1] {ECO:0000313|EMBL:AAF96758.1, ECO:0000313|Proteomes:UP000000584}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961
RC   {ECO:0000313|Proteomes:UP000000584};
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D., Ermolaeva M.D.,
RA   Vamathevan J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.,
RA   Utterback T., Fleishmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|PIRSR:PIRSR036917-2};
CC       Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR036917-2};
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DR   EMBL; AE003853; AAF96758.1; -; Genomic_DNA.
DR   PIR; B82409; B82409.
DR   RefSeq; NP_233246.1; NC_002506.1.
DR   RefSeq; WP_000772619.1; NZ_LT906615.1.
DR   AlphaFoldDB; Q9KL86; -.
DR   STRING; 243277.VC_A0860; -.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   EnsemblBacteria; AAF96758; AAF96758; VC_A0860.
DR   KEGG; vch:VC_A0860; -.
DR   PATRIC; fig|243277.26.peg.3476; -.
DR   eggNOG; COG0366; Bacteria.
DR   HOGENOM; CLU_022115_1_0_6; -.
DR   Proteomes; UP000000584; Chromosome 2.
DR   GO; GO:0042597; C:periplasmic space; IEA:InterPro.
DR   GO; GO:0004556; F:alpha-amylase activity; IEA:InterPro.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0030980; P:alpha-glucan catabolic process; IEA:InterPro.
DR   GO; GO:0009313; P:oligosaccharide catabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.80; Glycosidases; 2.
DR   InterPro; IPR014635; A_amylase_MalS.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR10357:SF215; ALPHA-AMYLASE 1; 1.
DR   PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1.
DR   Pfam; PF00128; Alpha-amylase; 2.
DR   PIRSF; PIRSF036917; Alph_amls_MalS; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE   4: Predicted;
KW   Calcium {ECO:0000256|PIRSR:PIRSR036917-2};
KW   Disulfide bond {ECO:0000256|PIRSR:PIRSR036917-4};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR036917-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000584};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           21..690
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004328405"
FT   DOMAIN          210..652
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   ACT_SITE        470
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036917-1"
FT   ACT_SITE        510
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036917-1"
FT   BINDING         331
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036917-2"
FT   BINDING         474
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036917-2"
FT   SITE            575
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036917-3"
FT   DISULFID        135..544
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036917-4"
SQ   SEQUENCE   690 AA;  77332 MW;  7C82C00C928FDF41 CRC64;
     MKLNALTLSL LSALALPALA SPASTANLTV ATTTNSRDFP LQADEPLVIP LTKGDYTLTI
     SGIGGDCPPV PEQEIKFNTP IALNCHVPTQ LPLSIRFTGD YAFQWQAQNN TLTFVRQTTK
     AAKTEFHRPI PNVSCEVYQG GEVTLDLASS FADGTELQDA MTGQVVTIEQ GKVRLTPSAN
     SGGLVLLEPK QTAKAEPKQP FTYRNANIYF VMVDRFYNAD PSNDGSYGRH KDGQEEIGTF
     HGGDLKGVIA KLDHIQSLGT DAIWLSPIVE QVHGFVGGGE KGSFPFYAYH GYWTRDFTKI
     DANFGKDEDL QTLVREAHRR GIKILMDAVI NHAGYATLAD LQQDAVQVVN APMLPERWND
     WKPSADENWH SFHQAIDYQS KNWQQWWGPD WVRAGLPGYP APGSSDITMN LAGLPDFRTE
     SPQAVTPPQW LLNNPGTRVV SKPNYTVADY LIEWQSDWVR RFGIDGFRID TVKHVEGEVW
     QRLKQRATES LAAWRKDNNQ SGEPFWMMGE VWGHGAYRSP YFDDGFDALI NFDIQKRMDN
     GAACLSQMAM VYRDYAQTLA KYPDFNPVSY MSSHDTELFF GRFKSLDMQR NAANALLLTP
     GAIQVYYGDE VAREAGPYAD DFHQGTRSDM PWEWNAERQA LLKHWQTLGQ FRQRHPAIGT
     GEHREIAQSN AYVFTRTLGE DKVVVAFVGR
//